Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran

Park, Ho-Young; Yu, A-Reum; Choi, In-Wook; Hong, Hee‐Do; Lee, Kwang Won; Choi, Hee‐Don

doi:10.1016/j.intimp.2013.06.013

Cited by 44 publications

(31 citation statements)

References 33 publications

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“…Among the various markers analyzed here, NO has been proposed to function as a potent tool in the immune response and a key molecule produced by macrophages for elimination of pathogens (Park et al, 2013). The fact there was enhanced NO production by RAW cells here clearly showed that IM-133N could probably affect transcription of various signaling genes normally regulated by NO.…”

Section: Discussionmentioning

confidence: 70%

“…For example, the methanol extract of Echinacea purpurea caused an increase in production of IL-6 and IL-10 by human and murine macrophages (Burger et al, 1997;Rininger et al, 2000). Similarly, a glycoprotein fraction from rice bran (Oryza sativa) strongly increased the production of TNF, IL-6 and IL-10 by RAW264.7 cells (Park et al, 2013). IM-133N contains the extracts of Symplocos recemosa and Prosopis glandulosa.…”

Section: Discussionmentioning

confidence: 99%

“…Macrophages and monocytes play significant roles in host defense mechanisms, as each, when activated, induce production of several cytokines that perform critical functions in a variety of immune responses (Park et al, 2013). Nitric oxide (NO) is synthesized by inducible nitric oxide synthase (iNOS) and mediates diverse functions, including vasodilation, neurotransmission and inhibition of platelet aggregation, immunoresponses and inhibition of extracellular matrix production.…”

Section: Introductionmentioning

confidence: 99%

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IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells

Varma¹,

Guruprasad

Satyamoorthy

et al. 2015

Journal of Immunotoxicology

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An indigenous herbal extract IM-133N containing extracts of Prosopis glandulosa Torr and Symplocos racemosa Roxb were evaluated for potential immunomodulatory effects using RAW264.7 and THP-1 cells. The incubation of the cells for 24 h with IM-133N over a dose range 0-125 mg/ml did not cause cytotoxicity that exceeded 10%. The results indicated that noncytotoxic doses of IM-133N effectively up-regulated iNOS, TNF, IL-6, IL-10, IL-8 and IFN gene expression in both the RAW264.7 and THP-1 cells. The results also indicated IM-133N elicited dose-related increases in nitric oxide (NO) and tumor necrosis factor (TNF)-production by RAW264.7 or THP-1 cells. These results demonstrated that IM-133N could stimulate NO and induced pro-inflammatory cytokine expression by monocytes/macrophages. As clinical studies have shown IM-133N to be an effective immunomodulator without any adverse effects, the results of the present study provide further support for the potential use of this agent as an immunostimulant or as an immunotherapy adjuvant.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells

Varma¹,

Guruprasad

Satyamoorthy

et al. 2015

Journal of Immunotoxicology

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“…in barley (Soerensen et al 1986;Laštovičková et al 2011), wheat (Breddam et al 1987) or rice (Park et al 2013). The special attention in research is concentrated to analysis of cereal allergenic glycoproteins.…”

Section: Cereal Glycoproteinsmentioning

confidence: 99%

Cerealn-glycoproteins Enrichment by Lectin Affinity Monolithic Chromatography

Flodrová

Bobáľová

Laštovičková

2016

Cereal Research Communications

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Research of cereal glycoproteins is important for understanding of their functional properties, their role during technological processing of cereals and it can serve as a valuable tool for the detection of cereal allergens. The main intention of this study was the screening of profile of water-soluble glycoproteins present in barley (Hordeum vulgare), wheat durum (Triticum durum) and spelt (Triticum spelta). Lectin monolithic HPLC column was used for rapid and effective enrichment of glycoprotein fractions. Captured glycoproteins were electrophoretically separated and analyzed by MALDI-TOF MS. Presented procedure resulted in identification of a group of N-glycoprotein candidates with affinity to lectin concanavalin A (ConA). Such molecules could have, among others, an allergenic potential. Majority of captured low-molecular-weight glycoproteins belong to alpha amylase/trypsin inhibitors family. However, most of the higher-molecular-weight proteins identified in lectin bound fractions have not been described as glycoproteins yet. Obtained results improved the knowledge about (glyco)protein content in cereal grain. The connection of lectin HPLC-GE-MS was proved as a convenient strategy for identification of cereal glycoproteins. Suggested method is universal and can be applied for various cereals and food-stuffs.

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“…Mice fed with bran oil have shown an immunostimulatory effect attributable to fatty acid-dependent eicosanoid production, particularly due to linoleic acid (Sierra, Lara-Villoslada et al 2005). Glycans present in a glycoprotein fraction from rice bran have improved the production of NO, TNF-α and various interleukins in RAW 264.7 murine macrophage cells (Park, Yu et al 2013). Immunostimulatory properties have also been pointed out for the arabinoxylan MGN-3, which in peripheral blood lymphocytes has induced the production of interferon-γ and TNF-α (Ghoneum and Jewett 2000).…”

Section: Immune Systemmentioning

confidence: 99%