Cereal<i>n</i>-glycoproteins Enrichment by Lectin Affinity Monolithic Chromatography

Flodrová, Dana; Bobáľová, Janette; Laštovičková, Markéta

doi:10.1556/0806.44.2016.019

Cited by 4 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, lectin affinity chromatography in various combinations with gel electrophoresis, liquid chromatography (LC), and MS techniques is the most widespread approach for glycoprotein analysis. 65,66 In quantitative proteomics, the iTRAQ method showed differential expression of selected barley allergenic proteins (e.g., pUP38, barwin) during food processing. 57 The methods used in proteomics are also applicable to glycoproteomics.…”

Section: Post-translational Modifications Of Barley Proteins and Diff...mentioning

confidence: 99%

“…The well-known proteomics approaches include MALDI-TOF, SDS-PAGE, 2D differential gel electrophoresis (DIGE), MS, two-dimensional gel electrophoresis (2DE), LC-MS/MS LC-ESI-MS/MS, iTRAQ, and peptide mass fingerprinting (PMF) have been applied in barley to determine PTMs. ,− Several published results confirmed that a combination of multiple omics approaches could be beneficial in identifying potential candidate genes, their pathways, and the covalent modification of proteins. Generally, lectin affinity chromatography in various combinations with gel electrophoresis, liquid chromatography (LC), and MS techniques is the most widespread approach for glycoprotein analysis. , In quantitative proteomics, the iTRAQ method showed differential expression of selected barley allergenic proteins (e.g., pUP38, barwin) during food processing …”

Section: Post-translational Modifications Of Barley Proteins and Diff...mentioning

confidence: 99%

See 1 more Smart Citation

Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley

Bobalova,

Strouhalova,

Bobal

2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Post-translational modifications (PTMs) of biomacromolecules can be useful for understanding the processes by which a relatively small number of individual genes in a particular genome can generate enormous biological complexity in different organisms. The proteomes of barley and the brewing process were investigated by different techniques. However, their diverse and complex PTMs remain understudied. As standard analytical approaches have limitations, innovative analytical approaches need to be developed and applied in PTM studies. To make further progress in this field, it is necessary to specify the sites of modification, as well as to characterize individual isoforms with increased selectivity and sensitivity. This review summarizes advances in the PTM analysis of barley proteins, particularly those involving mass spectrometric detection. Our focus is on monitoring phosphorylation, glycation, and glycosylation, which critically influence functional behavior in metabolism and regulation in organisms.

show abstract

Section: Post-translational Modifications Of Barley Proteins and Diff...mentioning

confidence: 99%

Section: Post-translational Modifications Of Barley Proteins and Diff...mentioning

confidence: 99%

Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley

Bobalova,

Strouhalova,

Bobal

2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…In fact, there are a number of lectin-based monolithic columns that are now commercially available. Concanavalin A (ConA) HPLC column (ProSwift ® ConA-1S monolith column by Thermo Scientific, Waltham, MA, USA) was used to design a protocol using lectin HPLC-GE-MALDI-TOF MS/MS that includes gel electrophoresis (GE) separation, MS detection, and consequential bioinformatic analysis to screen total N-glycoprotein "pool" in cereals [99]. The analysis was also done on water-soluble glycoproteins from wheat durum (Triticum durum), barley (Hordeum vulgare), and spelt (Triticum spelta).…”

Section: Lectin Affinity-based Monolithic Materialsmentioning

confidence: 99%

Recent Strategies for Using Monolithic Materials in Glycoprotein and Glycopeptide Analysis

Alla

Stine

2022

Separations

View full text Add to dashboard Cite

There is continuous effort towards developing monolithic materials as solid supports for the separation, enrichment, and digestion of glycoproteins. The intention of this review is to discuss and summarize work reported in this area during the period 2015–2021 as a follow-up to our prior review. Reports from the past three decades have already proven the advantages of monolithic materials, such as the ease with which they can be prepared and functionalized, their high permeability and low resistance to mass transfer, and their stability over a wide range of pH. Recent works on glycoprotein analysis introduce different strategies in using monolithic materials specifically in separation, enrichment, and identification of glycoproteins, glycopeptides, and free glycans. A majority of these are focused on boronic acid affinity-based technique and others on lectin affinity and HILIC-based techniques. There are also newly developed ligands that utilize different interactions with glycans, such as encapsulation into β-cyclodextrin vesicles, CH- or OH-π interactions with fullerenes, immunoaffinity with monoclonal antibodies, H-bonding interactions with metallophthalocyanines, coordination interactions with cobalt phthalocyanine tetracarboxylic acid, and hydrophilic interaction with cyclodextrin molecular tubes, zwitterionic iminodiacetic acid, and boric acid. Recent strategies for developing on-line, multidimensional systems use immobilized monolithic enzyme reactors (IMERs) for high-throughput glycoprotein analysis. These works serve as contributions to better understand glycan structure-function relationship, as glycoproteins are now widely accepted disease biomarkers.

show abstract

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

Harvey

2021

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This review is the ninth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2016. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation and arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented over 30 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show no sign of deminishing. © 2020 Wiley Periodicals, Inc.

show abstract

Cerealn-glycoproteins Enrichment by Lectin Affinity Monolithic Chromatography

Cited by 4 publications

References 25 publications

Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley

Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley

Recent Strategies for Using Monolithic Materials in Glycoprotein and Glycopeptide Analysis

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

Contact Info

Product

Resources

About