Immunostimulatory activity of potential probiotic yeast strains in the dorsal air pouch system and the gut mucosa

Abstract

Aims: To determine the immunostimulatory activity of 15 presumptive probiotic yeast strains in the dorsal air pouch system in comparison with their activity in the gut mucosa. Methods and Results: Presumptive probiotic yeast strains previously isolated from human gastrointestinal tract and Feta cheese were further characterized genotypically and biochemically. The Saccharomyces cerevisiae 982, Saccharomyces boulardii KK1 and Kluyveromyces lactis 630 strains exhibited in the air pouch increased polymorphonuclear cell influx and phagocytic activity as well as cytokine production with similar potency as the probiotics Ultra levure S. boulardii and Lactobacillus acidophilus NCFB 1748. Oral administration of these strains in mice results in differential activation of small intestine immune responses concerning IgA and cytokine production as well as Toll‐like receptor expression. Conclusion: Besides the Saccharomyces strains 982 and KK1, the K. lactis 630 strain could also be considered as a candidate probiotic. Significance and Impact of the Study: The air pouch model may be used as an alternative and rapid method for the discrimination and selection of potential probiotic yeast strains.

Probiotic Saccharomyces cerevisiae strains as biotherapeutic tools: is there room for improvement?

“…Strains of S. boulardii, S. cerevisiae, and Saccharomyces unisporus have also been isolated from alcoholic beverages such as Brazilian cachaça (Martins et al 2005), Ghanaian sorghum beer (Van der Aa Kühle et al 2005) and Northern Caucasus kefir (Diosma et al 2014), from Greek feta cheese (Kourelis et al 2010), Italian sourdough bread (Perricone et al 2014), and from children's feces (Kourelis et al 2010). All these strains have demonstrated probiotic potential, as indicated by optimal growth temperature at 37°C, resistance to GIT stresses, and other properties.…”

Fungi inhabiting the healthy human gastrointestinal tract: a diverse and dynamic community

“…Yeast fungi are known to be symbiotic with mammals. Saccharomyces yeasts may occur naturally in humans where they limit inflammatory response and increase immune health; these have also been used as probiotics ( Kourelis et al, 2010). Filamentous fungi, many of which decompose complex carbohydrates such as cellulose and lignin, have been studied in the digestive tracts of insects ( Lichtwardt, 2008) and the rumen of cattle ( Theodorou et al, 2005), and have been reported in human fecal samples ( Finegold et al, 1974).…”

Gluten induces coeliac-like disease in sensitised mice involving IgA, CD71 and transglutaminase 2 interactions that are prevented by probiotics

“…Saccharomyces Boulardii KK1 Strain, a Presumptive Probiotic, Improves Gluten-Induced Enteropathy Because probiotics have been shown to hydrolyse gliadin in vitro, 16 we examined whether presumptive probiotics with established immunoregulatory activity 14,15 have an effect in vivo in the gluten-induced mouse model for CD. The hydrolytic activity of L. paracasei DC412, DC205 or S. boulardii KK1 strains was first verified in vitro by preparing wheat flour fermentation in the presence of these strains.…”

“…All lactobacilli were stored at À801C in MRS broth with 25% glycerol, and KK1 cultures were maintained at 41C in a YEGP agar slope. 14,15 Yeast or lactobacilli were harvested at 18-24 h of culturing and resuspended at the appropriate concentration (CFU/ml) according to the McFarland standard.…”