Immunostimulation and trained immunity in marine fish larvae

Rojo-Cebreros, Angel Humberto; Ibarra‐Castro, Leonardo; Martínez-Brown, Juan Manuel

doi:10.1016/j.fsi.2018.05.044

Cited by 43 publications

(23 citation statements)

References 60 publications

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“…Glucose in the present study was enhanced as AMP was supplemented in shrimp diet and was in line with (Su et al, ). Aquatic organisms are able to live and survive in multiple environments because they have a special way of fighting against pathogen that surrounds them (Rojo‐Cebreros, Ibarra‐Castro, & Martínez‐Brown, ). Aquatic organisms are able to recover from disease infestations and acid repair worn out tissues due to some of the antioxidant defences they possess such as CAT (Liu et al, ).…”

Section: Discussionmentioning

confidence: 99%

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

et al. 2020

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This experiment was conducted to assess the effects of antimicrobial peptides at different levels (Diet 1 (0%), Diet 2 (0.1%), Diet 3 (0.2%), Diet 4 (0.4%), Diet 5 (0.6%) and Diet 6 (1%)), on growth, serum biochemical indices and antioxidant effect, feed utilization and disease resistance in Litopeneaus vannamei. There were four replicates in each group in the experiment (mean weight = 0.21 ± 0.00 g) and also fed with their respective diets for 8 weeks. Growth performance compared with the control group (0%) significantly increased at first and then decreased among treatment groups (p < .05) whereas the survival rate ranged from 78% to 96%. No significant differences were observed in terms of moisture, crude protein and ash content, but there was a significant increase in crude lipid (p < .05). In serum, acid phosphatase, alkaline phosphatase, polyphenol oxidase, triglyceride, glucose and total cholesterol changed as compared with 0%. Total antioxidant capacity, glutathione peroxidase, catalase, superoxide dismutase and malondialdehyde were also different from 0%. Disease resistance was increased in shrimp among treatment groups with 0.4% recording the lowest mortality percentage of 37% after the challenge test. The results from the present study suggested that supplementation of AMP at 0.4% in shrimp diet can improve growth performance, antioxidants activities and innate immune response of Pacific whiteleg shrimp.

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Section: Discussionmentioning

confidence: 99%

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

et al. 2020

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“…High mortality was one of the important limiting factors during fish larvae culture, and more attention has been paid to enhance larval health to improve the survival rate ( Rojo-Cebreros et al, 2018 ). Allicin has been used as immunostimulants to enhance the immune response in mice ( Bruck et al, 2005 ), chicken ( Wang et al, 2017 ), pig ( Lan et al, 2017 ) and many fish species ( Nya and Austin, 2011 ; Abdel-Daim et al, 2015 ; Xu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Compared to juvenile and adult fish, fish larvae are relatively fragile and susceptible to environmental stress due to the incomplete tissue development and immune system ( Cai et al, 2016 ), and thus high mortality rate always occurs during the larval culture period ( Rojo-Cebreros et al, 2018 ). Large yellow croaker ( Larimichthys crocea ) is a carnivorous marine fish widely cultivated in southeastern China, with the largest production among all marine fish species in China, due to its delicious taste ( Ai et al, 2008 ; Xie et al, 2011 ; Zhao et al, 2013 ; Liu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Dietary Allicin Improved the Survival and Growth of Large Yellow Croaker (Larimichthys crocea) Larvae via Promoting Intestinal Development, Alleviating Inflammation and Enhancing Appetite

et al. 2020

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A 30-day feeding experiment was conducted to investigate effects of dietary allicin on survival, growth, antioxidant capacity, innate immunity and expression of inflammatory and appetite related genes in large yellow croaker larvae. Four iso-nitrogenous (53% crude protein) and iso-lipidic (19% crude lipid) diets were formulated via supplementing graded levels of allicin (0.0 (the control), 0.005, 0.01, and 0.02% dry diet, respectively). Results showed that, among dietary treatments, larvae fed the diet with 0.005% allicin had the highest survival rate (SR) ( P < 0.05), while larvae fed the diet with 0.01% allicin had the highest specific growth rate (SGR) ( P < 0.05). Activities of α-amylase in both pancreatic (PS) and intestine segments (IS) of larvae fed the diet with 0.01% allicin were significantly lower than that in the control ( P < 0.05). On the other hand, the supplementation of 0.01% allicin in diets significantly increased activities of alkaline phosphatase (AKP) and leucine aminopeptidase (LAP) in the intestinal brush border membrane (BBM) of larvae than the control ( P < 0.05), indicating the promoting roles of allicin on fish larval intestinal development. Moreover, compared to the control, both the nitric oxide (NO) content and the activity of nitric oxide synthase (NOS) were significantly up-regulated in larvae fed the diet with 0.005% allicin, and catalase (CAT) were significantly upregulated in larvae fed the diet with 0.02% allicin ( P < 0.05). Transcriptional levels of pro-inflammatory genes including cyclooxygenase-2 ( cox-2 ), interleukin-1β ( il-1 β) and interleukin-6 ( il-6 ) significantly decreased with increasing allicin, compared to the control. The expression of appetite genes including npy , ghrelin and leptin significantly increased with the prolonged fasting period, and dietary allicin supplementation significantly increased the transcriptional level of neuropeptide Y ( npy ) at 0.01%, while increased the transcriptional level of leptin in larvae at 0.02% dosages ( P < 0.05). These results showed that the supplementation of 0.005% – 0.01% allicin in diets could improve the survival and growth of large yellow croaker larvae probably by promoting intestinal development, alleviating inflammation and enhancing appetite.

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“…vitamin C and E), microorganisms (i.e., probiotic bacteria), prebiotics (Bio-Mos R ), hormones (i.e., thyroxine), biologically active compounds (i.e., antimicrobial peptides, lectins, bovine lactoferrin), medicinal herbs [i.e., Astragalus membranaceus and Lonicera japonica (honeysuckle) extracts], amino acids (i.e., DL-arginine), poly-β-hydroxybutyrate and organic pigments (i.e., carotenoid and astaxanthin) has also been studied in various aquaculture species (44,(56)(57)(58)(59). However, data from application of immunostimulants in fish larvae are limited [reviewed by (18,60)]. Nevertheless, the existing data point in the direction of considerable benefit and little detrimental effect to the developing animal (44).…”

Section: Figure 5 |mentioning

confidence: 99%

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

et al. 2020

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Vibrio anguillarum causes high mortality in European sea bass (Dicentrarchus labrax) larviculture and is a hindering factor for successful sustainable aquaculture of this commercially valuable species. Priming of the innate immune system through administration of immunostimulants has become an important approach to control disease outbreaks in marine fish larviculture. This study was conducted to evaluate immunostimulation by Escherichia coli HSP70 (DnaK) in axenic European sea bass larvae in order to protect the larvae against vibriosis. DnaK stimulates the immune response in crustaceans and juvenile fish against bacterial infections. The use of axenic fish larvae allows to study immunostimulation in the absence of an interfering microbial community. At 7 days post-hatching, larvae received a single dose of alginate encapsulated recombinant DnaK. Two non-treated control groups in which animals either received empty alginate microparticles (C1) or no alginante microparticles (C2 and C3) were included in the study. Eighteen hours later, all larvae, except the ones from group C3 (non-infected control) were challenged with V. anguillarum (10 5 CFU, bath infection). Mortality was daily recorded until 120 h post infection and at 18, 24, and 36 h post infection, larvae were sampled for expression of immune related genes. Results showed that V. anguillarum induced an immune response in axenic sea bass larvae but that the innate immune response was incapable to protect the larvae against deadly septicaemic disease. In addition, we showed that administration of alginate encapsulated DnaK to axenic European sea bass larvae at DAH7 resulted in a significant, DnaK dose dependent, upreglation of immune sensor, regulatory and effector genes. Significant upregulation of cxcr4, cas1 and especially of hep and dic was correlated with significant higher survival rates in V. anguillarum infected larvae. In the future recombinant DnaK might perhaps be used as a novel immunostimulant in sea bass larviculture.

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Immunostimulation and trained immunity in marine fish larvae

Cited by 43 publications

References 60 publications

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp,Litopenaeus vannamei

Dietary Allicin Improved the Survival and Growth of Large Yellow Croaker (Larimichthys crocea) Larvae via Promoting Intestinal Development, Alleviating Inflammation and Enhancing Appetite

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

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