Immunoserology of European seabass (Dicentrarchus labrax) and white grouper (Epinephelus aeneus) as a non-lethal diagnostic tool for viral nervous necrosis

Tarrab, Koby; Ravid-Peretz, Shay; Ucko, Michal

doi:10.1007/s10499-018-0307-6

Cited by 8 publications

(16 citation statements)

References 37 publications

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“…These results agree with a recent study performed by Tarrab et al. (), where similar results were obtained since the non‐lethal tissues analysed, blood, fin clips and testis, showed a lower virus concentration when compared to the brain. It was also shown in another study that the tissue from the brain and eye had the highest titre and copy number, and the gills, spleen and kidney, the non‐nervous tissues, presented lower viral titres (Kim, Kim, Kim, Kim, & Oh, ).…”

Section: Discussionsupporting

confidence: 93%

“…These results are similar to those reported by Tarrab et al. (), who verified an antibody response in fish as early as 5 dpi. The early onset of anti‐NNV IgM response may be a useful tool for establishing the NNV‐free status of fish to avoid new VER outbreaks and restricting the disease spread between farms with the movement of fish.…”

Section: Discussionsupporting

confidence: 92%

“…The antibody titres reported in this study are lower than those reported by Tarrab et al. (). These lower antibody titres can be related to the lower mortality values, since this was a sub‐clinical infection as mentioned before.…”

Section: Discussioncontrasting

confidence: 87%

“…Tarrab et al. () recently reported on the use of immunoserology of European seabass and white grouper ( Epinephelus aeneus ) as a non‐lethal diagnostic tool for VNN.…”

Section: Introductionmentioning

confidence: 99%

“…There is also growing concern about killing animals from an ethical point of view (Blessing, Marshall, & Balcombe, 2010;Drennan et al, 2007;Henderson, Stevens, & Lee, 2016). There is, therefore, increasing interest in using non-lethal samples for diagnostics, and different sampling methods have been used to screen fish for exposure to viral (Collet et al, 2015;Monaghan, Thompson, Adams, & Bergmann, 2014;Tarrab, Ravid-Peretz, & Ucko, 2018), bacterial (Monte, Urquhart, Secombes, & Collet, 2016;Tavares et al, 2015) and parasitic (Ek-Huchim, Jiménez-Garcia, Pérez-Vega, & Rodríguez-Canul, 2012;Fox, Palenzuela, & Bartholomew, 2000;Krkošek, Morton, & Volpe, 2005) pathogens. Different types of material that have been assessed for this purpose include blood and biopsies (Altinok, Grizzle, & Liu, 2001;Cornwell et al, 2013).…”

mentioning

confidence: 99%

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Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods

Ferreira

Costa

Macchia

et al. 2019

Journal of Fish Diseases

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One of the major disease threats affecting the Mediterranean aquaculture industry is viral encephalopathy and retinopathy (VER). The target organs for Betanodavirus detection are the brain and eyes, obtained through lethal sampling. This study aimed to evaluate the efficacy and suitability of non‐lethal samples for detecting Betanodavirus in European seabass (Dicentrarchus labrax). European seabass juveniles were infected with Betanodavirus, by either an intramuscular injection or immersion (107 TCID50/ml and 106 TCID50/ml, respectively), and samples collected 7, 15 and 30 days post‐infection (dpi). The brain was collected as a lethal sample, and gills, caudal fin and blood as non‐lethal tissues for detecting Betanodavirus by quantitative reverse transcription PCR (RT‐qPCR). The presence of virus in non‐lethal tissues was inconsistent, with lower viral loads than in the brain. For blood, higher viral loads were detected in intramuscular‐infected fish at 15 dpi until the end of the challenge. Serum antibodies against Betanodavirus were assessed using an enzyme‐linked immunosorbent assay (ELISA). Antibodies were detected as early as 7 dpi, with higher mean antibody titres at 15 and 30 dpi. The presence of Betanodavirus‐specific antibodies indicates that this is a suitable evaluation method for detecting early stages of the infection.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 87%

“…Tarrab et al. () recently reported on the use of immunoserology of European seabass and white grouper ( Epinephelus aeneus ) as a non‐lethal diagnostic tool for VNN.…”

Section: Introductionmentioning

confidence: 99%