Immunosensors for diagnostic applications

Cruz, Hélder; Rosa, Carla C.; Oliva, Abel

doi:10.1007/s00436-001-0559-2

Cited by 40 publications

(20 citation statements)

References 10 publications

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“…This assay involves the use of a receptor (mostly an antibody), a disease specific antibody and a transducer that converts a biological interaction into a measurable signal (Cruz et al, 2002). These biosensors are frequently coupled to sophisticated instrumentation to produce highly-specific analytical tools, most of which are still in use only for the research and development purpose due to the high cost of instrumentation, high cost of individual sample analysis, and the need for highly trained persons to oversee the testing.…”

Section: Biosensorsmentioning

confidence: 99%

Biotechnological tools for diagnosis of equine infectious diseases

Prasad¹,

Brar²,

Ikbal³

et al. 2016

JEBAS

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Rapid diagnosis of infectious diseases and appropriate treatment with in time are important steps that promote optimal clinical outcomes and general public health. Today there is large number of new technologies such as nanotechnology, biosensors, and microarray techniques, are being developed and used as diagnostic tools for equine infectious diseases. Nucleic acid based techniques such as polymerase chain reaction (PCR) have become conventional tools in veterinary research and plays an important role in specific typing determinations as well as for rapid screening of ample numbers of samples at the time of equine disease outbreaks. Other biotechnological techniques are populous to be used in the coming times as they can enhance diagnostic efficacy in less time and cost as compared to conventional techniques. This review focuses on biotechnological tools available for equine diseases diagnosis and its applications hold great promise for improving the speed and accuracy of diagnostics for equine infectious diseases.

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Section: Biosensorsmentioning

confidence: 99%

Biotechnological tools for diagnosis of equine infectious diseases

Prasad¹,

Brar²,

Ikbal³

et al. 2016

JEBAS

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“…Immunosensors are based on the immunochemical reaction comprising antigen or antibody as the recognition element, which is spatially in contact with a transducer surface [42]. Immunosensor‐based devices can be used to detect immunocomplex formation by measuring changes in resistance, current, refractive index, capacitance, etc.…”

Section: Microfluidics‐based Biosensors For Poc Diagnosticsmentioning

confidence: 99%

Microfluidic‐integrated biosensors: Prospects for point‐of‐care diagnostics

Kumar

Ali

et al. 2013

Biotechnology Journal

169

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There is a growing demand to integrate biosensors with microfluidics to provide miniaturized platforms with many favorable properties, such as reduced sample volume, decreased processing time, low cost analysis and low reagent consumption. These microfluidics-integrated biosensors would also have numerous advantages such as laminar flow, minimal handling of hazardous materials, multiple sample detection in parallel, portability and versatility in design. Microfluidics involves the science and technology of manipulation of fluids at the micro- to nano-liter level. It is predicted that combining biosensors with microfluidic chips will yield enhanced analytical capability, and widen the possibilities for applications in clinical diagnostics. The recent developments in microfluidics have helped researchers working in industries and educational institutes to adopt some of these platforms for point-of-care (POC) diagnostics. This review focuses on the latest advancements in the fields of microfluidic biosensing technologies, and on the challenges and possible solutions for translation of this technology for POC diagnostic applications. We also discuss the fabrication techniques required for developing microfluidic-integrated biosensors, recently reported biomarkers, and the prospects of POC diagnostics in the medical industry.

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“…Specificity arises from the molecular recognition of target analytes by antibodies to form a stable complex on the immunosensor surface [1,2]. On the other hand, sensitivity depends on several factors including the use of high affinity analyte-specific antibody(ies), their orientation after being immobilized on the immunosensor surface and the appropriate detection system for measurement of the signal generated via a transducer [3]. Many types of immunosensors have been developed according to the mode of detection.…”

Section: Introductionmentioning

confidence: 99%

Signal Generation at an Electrochemical Immunosensor via the Direct Oxidation of an Electroactive Label

2006

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In this paper, we report the development of an electrochemical immunosensor with a direct signal generation scheme. To achieve this, ferrocenecarboxylic acid was conjugated as an electroactive label to an immunocomplex immobilized on an electrochemically pretreated glassy carbon electrode. The pregnancy hormone and tumor marker, human chorionic gonadotrophin (hCG), was employed as a model analyte. Direct oxidation of ferrocenecarboxylic acid facilitated a signal generation scheme for the quantification of hCG at the immunosensor. Evaluation of the immunosensor performance has yielded statistically acceptable agreement in hCG levels present in several human serum samples compared to those reported by a Sydney hospital.

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Immunosensors for diagnostic applications

Cited by 40 publications

References 10 publications

Biotechnological tools for diagnosis of equine infectious diseases

Biotechnological tools for diagnosis of equine infectious diseases

Microfluidic‐integrated biosensors: Prospects for point‐of‐care diagnostics

Signal Generation at an Electrochemical Immunosensor via the Direct Oxidation of an Electroactive Label

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