Microfluidic‐integrated biosensors: Prospects for point‐of‐care diagnostics

Kumar, Suveen; Kumar, Saurabh; Ali, Md. Azahar; Anand, Pinki; Agrawal, Ved Varun; John, Richard R.; Maji, S.; Malhotra, Bansi D.

doi:10.1002/biot.201200386

Cited by 169 publications

(98 citation statements)

References 72 publications

(64 reference statements)

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“…Biosensors are becoming more important to human disease assessment and promise to revolutionize pointof-care diagnosis (Kumar et al 2013;Wang 2006). For example, the use of sensor technologies to identify biomarkers associated with wound pathology at a biochemical or cellular level has received increased attention as recently reviewed (Mohanty and Kougianos 2006).…”

Section: Introductionmentioning

confidence: 99%

Human neutrophil elastase peptide sensors conjugated to cellulosic and nanocellulosic materials: part I, synthesis and characterization of fluorescent analogs

et al. 2016

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Here we describe the synthesis and characterization of peptide conjugated cellulose and nanocellulose materials as sensors for fluorescent detection of human neutrophil elastase (HNE). The cellulose sensor surfaces selected are filter paper (FP) and print cloth (PC) fabric, which are composed of processed cotton fibers. The nanocellulose based sensors have transducer surfaces comprised of cellulose nanocrystals (wCNC) and microfibrillated cellulose (MFC) derived from wood that are fabricated as wood nanocellulose composites (wNCCs) consisting of blended quantities of nanocrystalline and MFC at 66/33 and 50/50 ratios. These ratios were selected to examine the effect of significantly different CNC loadings had on surface area and peptide uptake. The fluorescent peptide HNE substrate, n-succinyl-Ala-Pro-Ala-4-amido-7-methylcoumarin (Pep) was attached to both cellulosic and nanocellulosic matrices and conjugated peptide analogs were confirmed by mass spectrometry (MS) and infrared (IR). The nanocellulose biosensors wCNCPep (3c) and wNCC-Pep (4c, 66/33 and 5c, 50/50) have higher levels of peptide incorporation than the cellulosic biosensors FP-Pep (1c) and PC-Pep (2c). The range of incorporation for the cellulosic sensors is 7-25 lg/mg and for the nanocellulose sensors 30-80 lg/mg. The degree of substitution of peptide was found to be in the order of approximate number of peptides per 200 anhydroglucose residues, 1 in PC-Pep (2c), 2 in FP-Pep (1c), 4 in wNCC-Pep (5c, 50/50), 6 in wNCC-Pep (4c, 66/33), and 12 in wCNC-Pep (3c). The specific surface areas of the sensors ranged from 0.016 to 261 m 2 g -1 and correlated with degree of substitution of peptide on the cellulosic and nanocellulosic surfaces. Of the cellulose and nanocellulose biosensors, the wCNC-Pep (3c) has the highest level of peptide incorporation and the highest specific surface area, which makes it the preferred sensor matrix for human neutrophil elastase.

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Section: Introductionmentioning

confidence: 99%

Human neutrophil elastase peptide sensors conjugated to cellulosic and nanocellulosic materials: part I, synthesis and characterization of fluorescent analogs

et al. 2016

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“…The detection of cancer via biomarkers has been recently proposed 13. In this context, the biomarkers, such as interleukin‐8 (IL‐8), interleukin‐6 (IL‐6), vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) are currently being used for detection of oral cancer 14, 15, 16, 17.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection

et al. 2015

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Results of the studies are reported relating to application of the silanized nanostructured zirconia, electrophoretically deposited onto indium tin oxide (ITO) coated glass for covalent immobilization of the monoclonal antibodies (anti‐CYFRA‐21‐1). This biosensing platform has been utilized for a simple, efficient, noninvasive, and label‐free detection of oral cancer via cyclic voltammetry technique. The results of electrochemical response studies conducted on bovine serum albumin (BSA)/anti‐CYFRA‐21‐1/3‐aminopropyl triethoxy silane (APTES)/ZrO2/ITO immunoelectrode reveal that this immunoelectrode can be used to measure CYFRA‐21‐1 (oral cancer biomarker) concentration in saliva samples, with a high sensitivity of 2.2 mA mL ng−1, a linear detection range of 2–16 ng mL−1, and stability of six weeks. The results of these studies have been validated via enzyme‐linked immunosorbent assay.

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“…Besides this, the testing can be performed near the vicinity of a patient and hence these devices have recently gained much attention in health care. (Gervais et al 2011;Kumar et al 2013a;Kumar et al 2013b;Luppa et al 2011) Electrochemical sensors are known to play an important role in on-going transition towards the paper based POC diagnostic devices. The electrochemical techniques offer high sensitivity, high signal-to-noise ratio, portability and fast response time.…”

Section: Introductionmentioning

confidence: 99%