Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium

Wąsowicz, Michał; Milner, Małgorzata; Radecka, Dorota; Grzelak, Krystyna; Radecka, Hanna

doi:10.3390/s100605409

Cited by 22 publications

(32 citation statements)

References 38 publications

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“…Several successful applications of EIS for detection of different biomolecules such as: his-tagged antibody and amyloid b-peptide have been reported by our group [18,[35][36][37][38].…”

Section: Determination Of Ppv Virions With the Electrochemical Immunomentioning

confidence: 99%

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

et al. 2011

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This paper concerns the development of an immunosensor for detection of Plum pox virus in plant extracts. The sensor was based on gold electrodes modified with: 1,6-hexanedithiol, gold nanoparticles, anti-PPV IgG polyclonal antibody and BSA. It was used for determination of the virus in extracts from plum (Prunus domestica) and tobacco (Nicotiana benthamiana) leaves. The proposed electrochemical impedance spectroscopy (EIS) immunosensor displayed a very good detection limit of 10 pg/mL and a wide dynamic range from 10 pg PPV/ mL to 200 pg/mL. The presence of extracts from plant materials has no influence on the immunosensors response. The immunosensor was capable of discriminating between samples from healthy plants and samples containing 0.01 % of extract from infected plant material. The conditions for immunosensor regeneration are also presented.

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“…Several successful applications of EIS for detection of different biomolecules such as: his-tagged antibody and amyloid b-peptide have been reported by our group [18,[35][36][37][38].…”

Section: Determination Of Ppv Virions With the Electrochemical Immunomentioning

confidence: 99%

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

et al. 2011

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“…The most popular mode for analyzing electrochemical impedance data is the plot of the imaginary impedance component versus real impedance component at each excitation frequency the Nyquist plot . Numerous impedimetric immunosensors have been already reported [7,30,31,38]. This approach has been successfully applied for detection of fragments of H5 hemagglutinin from avian inluenza viruses [9,10].…”

Section: Methods Of Detection Of Immunoreaction: Selected Examplementioning

confidence: 99%

“…The presence of disulide or thiol group allow the F ab' 2 or F ab' fragments to covalent self-assembling on the gold nanoparticle surface [33,34]. This approach has been successfully applied for development of immunosensor destined for selective binding of antigen rSPI2-His present in the sample solution by F ab' fragment of antibody immobilized on a surface of the electrode using electrochemical impedance spectroscopy EIS and surface plasmon resonance SPR [30] as well as for selective detection of hemagglutinin from avian inluenza virus H5N1 [9,10]. The proteins " and G possessing high ainity toward the Fc part of immunoglobulins are frequently used for their covalent and oriented immobilization on the electrode surface [35].…”

Section: Electrochemical Immunosensors Methods For Immobilizatimentioning

confidence: 99%

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

Radecka¹,

Radecki²

2016

Steps Forwards in Diagnosing and Controlling Influenza

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Abstract"vian inluenza viruses "IVs could cause severe diseases and, as the consequence, serious economic losses as well as a risk for potential transmission to humans. Therefore, the detection of viruses and their fragments or speciic DN" sequences becomes an important approach in molecular diagnosis. Here, we present electrochemical genosensors devoted for detection of inluenza virus H5N1 gene sequence. We focus our atention on ion-channel mechanism, E-DN" sensors, and genosensors based on redox-active layer. The novel dual DN" electrochemical sensor with "signal-of" and "signal-on" architecture for simultaneous detection of two diferent sequences of DN" derived from avian inluenza virus type H5N1 by means of one electrode is presented. Immunosensors are also adequate analytical devices for detection of pathogens since antibodies are natural receptors responsible for binding of antigens. Thus, the binding selectivity and eiciency are naturally high. The immunosensors presented could be divided into two main groups: ion-channel mimetic and based on redox-active monolayer.

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“…Therefore, alternative sensors have been developed for specific detection of His-tagged proteins. They include gold nanorod sensors using surface plasmon resonance (Wasowicz et al 2008), impedance spectroscopy (Wasowicz et al 2010), and field effect transistors (Liu et al 2010). These detection systems are sufficiently sensitive (detection limits, *5-60 lg/ml), and fast (10-30 min), but these are based on nontraditional equipment for a molecular biology laboratory.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of 6×-histidine-labeled recombinant proteins by immunochromatography using dye-labeled cellulose nanobeads

Choi

Lee

et al. 2014

Biotechnol Lett

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A rapid and easy immunochromatography assay using dye-labeled cellulose nanobeads (CNBs) was developed to detect proteins with hexa-histidine tag (His-tag) to characterize recombinant proteins during purification. Recombinant ATG8 protein was used as a His-tagged protein, and ATG8-conjugated CNBs (A-CNBs) were prepared. The original ATG8 in the sample solution competed with A-CNBs for anti-His-tag antibodies spotted on to the strip resulting in an inverse relationship between ATG8 concentration and the colorimetric signal. The usefulness of this method was shown by adding ATG8 to a 1% Escherichia coli extract. In addition, this assay can be used to detect other His-tagged proteins without protein-specific antibodies. Because the identification of fractions containing His-tagged proteins by western blotting or ELISA is labor-intensive and expensive, our method provides an efficient and cheaper alternative.

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Immunosensor Incorporating Anti-His (C-term) IgG F(ab’) Fragments Attached to Gold Nanorods for Detection of His-Tagged Proteins in Culture Medium

Cited by 22 publications

References 38 publications

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

Rapid detection of 6×-histidine-labeled recombinant proteins by immunochromatography using dye-labeled cellulose nanobeads

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