Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Luong, John H. T.; Vashist, Sandeep Kumar

doi:10.1016/j.bios.2015.11.053

Cited by 29 publications

(11 citation statements)

References 86 publications

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“…This might be because at low concentration of 20 μg mL -1 , GNPs dispersed better compared to that at higher concentrations and thus, the large surface area was able to be deployed for better ECL performance. 23 Another experimental parameter evaluated was the concentration of anti-cTnT.…”

Section: Selection Of the Materials For The Biosensor Developmentmentioning

confidence: 99%

A Label-free Cardiac Troponin T Electrochemiluminescence Immunosensor Enhanced by Graphene Nanoplatelets

et al. 2019

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In this study, a direct and label-free immunosensor was designed and constructed by modifying the screen-printed electrode with graphene nanoplatelets (GNPs) for the detection of the cardiac troponin T (cTnT). Firstly, GNPs were drop-casted onto carbon working electrode. Monoclonal cTnT antibodies were then immobilized on the GNPs via physical adsorption; finally, BSA was introduced to block non-specific binding sites. The detection of cTnT was performed using an electrochemiluminescence (ECL) technique with tris(bipyridine)ruthenium(II) chloride ([Ru(bpy)3]Cl2) used as a luminophore and TPrA (tripropylamine) as a co-reactant. The ECL intensity was demonstrated to be directly proportional to the cTnT concentration where a linear range from 100 pg mL-1 to 5 fg mL-1 of the cTnT detection was established. An extremely low limit of detection was achieved to be 0.05 fg mL-1 with an outstanding specificity. Additionally, this immunosensor showed excellent percentage recovery for real samples analyses in artificially spiked human serum.

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Section: Selection Of the Materials For The Biosensor Developmentmentioning

confidence: 99%

A Label-free Cardiac Troponin T Electrochemiluminescence Immunosensor Enhanced by Graphene Nanoplatelets

et al. 2019

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“…It is well known, when the materials size are brought to nanoscale sizes they suddenly display very different properties compared to their original properties as a bulky material (Vigneshvar et al, 2016). A variety of nanomaterials has been synthesized using different preparation methods Sadeghian et al, 2017;Ahmad et al, 2017aAhmad et al, , 2017bAhmad et al, , 2017cAshley et al, 2017;Luong and Vashist, 2017;Shrestha et al, 2017). Among diverse nanomaterials architectures, NWs are highly functional structures and offer unique properties due to their one dimensionalities.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in nanowires-based field-effect transistors for biological sensor applications

Ahmad

Mahmoudi

Ahn

et al. 2018

Biosensors and Bioelectronics

132

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Nanowires (NWs)-based field-effect transistors (FETs) have attracted considerable interest to develop innovative biosensors using NWs of different materials (i.e. semiconductors, polymers, etc.). NWs-based FETs provide significant advantages over the other bulk or non-NWs nanomaterials-based FETs. As the building blocks for FET-based biosensors, one-dimensional NWs offer excellent surface-to-volume ratio and are more suitable and sensitive for sensing applications. During the past decade, FET-based biosensors are smartly designed and used due to their great specificity, sensitivity, and high selectivity. Additionally, they have the advantage of low weight, low cost of mass production, small size and compatible with commercial planar processes for large-scale circuitry. In this respect, we summarize the recent advances of NWs-based FET biosensors for different biomolecule detection i.e. glucose, cholesterol, uric acid, urea, hormone, proteins, nucleotide, biomarkers, etc. A comparative sensing performance, present challenges, and future prospects of NWs-based FET biosensors are discussed in detail.

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“…Current methods for the detection of tumor markers, including electrochemistry [ 3 , 4 , 5 ], fluorescence [ 6 , 7 , 8 ], chemiluminescence [ 9 , 10 ], and graphene-based biosensors [ 11 , 12 , 13 ], provide sensitive and specific techniques. However, some of them need expensive reagents, bulky instruments, and lack the traits of real-time and label-free detection.…”

Section: Introductionmentioning

confidence: 99%

Determination of Tumor Marker Carcinoembryonic Antigen with Biosensor Based on Optical Quantum Weak Measurements

Guan

Wang

et al. 2018

Sensors

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A phase-sensitive weak measurement biosensor was proposed for the detection of carcinoembryonic antigen (CEA), one common category of tumor markers. The total internal reflection (TIR) at the interface of the prism without precious metal coating was exploited to introduce the phase delay between horizontal and vertical polarizations, which can be determined through the central wavelength shift of output spectra for the sensing of the refractive index of the sample. In the weak measurement analysis, the specific binding reaction of tumor markers with a refractive index change on the surface of the prism can be monitored in real time through the central wavelength shift. With the specific absorption measurement, the feasibility of this weak measurement-based biosensor was experimentally demonstrated. We provide a low cost and convenient approach for tumor marker detection.

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Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Cited by 29 publications

References 86 publications

A Label-free Cardiac Troponin T Electrochemiluminescence Immunosensor Enhanced by Graphene Nanoplatelets

A Label-free Cardiac Troponin T Electrochemiluminescence Immunosensor Enhanced by Graphene Nanoplatelets

Recent advances in nanowires-based field-effect transistors for biological sensor applications

Determination of Tumor Marker Carcinoembryonic Antigen with Biosensor Based on Optical Quantum Weak Measurements

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