Immunoregulatory activity by daucosterol, a β-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice

Lee, Jue-Hee; Lee, Ju Young; Park, Ji Hye; Jung, Hye Sil; Kim, Ju Sun; Kang, Sam Sik; Kim, Yeong Shik; Ye, Han

doi:10.1016/j.vaccine.2007.01.108

Cited by 111 publications

(87 citation statements)

References 23 publications

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“…On the Th2 side, no differences were seen in IL-4 and IL-10 production in the sitosterol and sitostanol conditions compared with control conditions. Breytenbach et al (14) showed that a plant sterol/sterolin mixture induced Th1 activity in HIV patients, whereas others reported similar effects for plant sterols in mice (16,32,33). Calpe-Berdiel et al (16) have shown that dietary plant sterol supplementation increased the production of Th1 cytokines IL-2 and IFN␥ during acute a-septic inflammation in mice, whereas no changes in the production of IL-4 and IL-10 were observed.…”

Section: Discussionmentioning

confidence: 99%

TLR2 Activation Is Essential to Induce a Th1 Shift in Human Peripheral Blood Mononuclear Cells by Plant Stanols and Plant Sterols

Brüll

Mensink

Hurk

et al. 2010

Journal of Biological Chemistry

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Plant sterols may induce a Th1 shift in humans. However, whether plant stanols have similar effects as well as the underlying mechanism are unknown. We have now shown that (like sitosterol) sitostanol, both 4-desmethylsterols, induces a Th1 shift when added in vitro at physiological concentrations to human PBMCs. This conclusion was based on a higher IFN␥ production, with no change in the production of IL-4 and IL-10. ␣-Amyrin, a 4.4-dimethylsterol, had comparable effects. Because 4.4-dimethylsterols cannot activate transcription factor LXR, this finding indicates that LXR activation was not involved. Sitosterol and sitostanol did not alter the production of IL-12 and IL-18 in PBMCs as well as in monocyte-derived U937 cells, suggesting that plant sterols directly affect T-helper cells, without activating APCs. However, in PBMCs treated with a TLR2 blocker (T2.5), IFN␥ production was completely inhibited, whereas blocking TLR4 with HTA125 had no such effect. To confirm these findings, PBMCs from TLR2 ؊/؊ mice were cultured in the presence of sitosterol and sitostanol. In these cells, no Th1 shift was observed. Our results, therefore, indicate that TLR2 activation is essential to induce a Th1 shift in human PBMCs by plant stanols and plant sterols.The human body responds to infectious challenges by a wide variety of cellular and humoral responses, in which numerous cells and factors play a role. During these responses, CD4 ϩ

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Section: Discussionmentioning

confidence: 99%

TLR2 Activation Is Essential to Induce a Th1 Shift in Human Peripheral Blood Mononuclear Cells by Plant Stanols and Plant Sterols

Brüll

Mensink

Hurk

et al. 2010

Journal of Biological Chemistry

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“…1) from subfraction B-20-23. The compounds were identified as daucosterol, formononetin, astragaloside I, and astragaloside IV by NMR and LC/MS and comparison with literature data (Hirotani et al, 1994;Kim et al, 1996;Lee et al, 2007).…”

Section: Preparing Extract and Isolation Constituents From Armentioning

confidence: 91%

Suppressive Actions of Astragali Radix (AR) Ethanol Extract and Isolated Astragaloside I on HCl/ethanol-Induced Gastric Lesions

Jeong¹,

Lee²,

Lee³

et al. 2009

Biomolecules and Therapeutics

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-Roots of Astragalus membranaceus (A. membranaceus) (Astragali Radix, AR) has been used as a herbal medicine for gastrointestinal disorders in China, Korea, Japan, and other Asia countries. In this study we investigated the effects of the AR 70% ethanol extract and compounds isolated from AR on gastritis in rats and growth of human gastric cancer cells. AR 70% ethanol extract showed the potent acid-neutralizing capacities and partly cytotoxicity of Helicobacter pylori and human AGS gastric cancers cell. Astragaloside I and daucosterol, which were isolated from AR, significantly inhibited HCl/ethanol-induced gastric lesions. In addition, daucosterol increased the mucus content to almost the same as the positive control. The results of this study suggest that astragaloside I and daucosterol may be good candidates for the development of new drugs or neutraceuticals which can be used for the treatment or prevention of gastritis.

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“…clinopodioides [44] Immunoregulatory effect [150] Anti-inflammatory activity [151]; anti-inflammatory in ear edema assay [152]; topical anti-inflammatory activity in the mouse ear edema model [153]; weak 5-LOX inhibitory activity [154] DPPH and ABTS; scavenging effect [155]; antioxidant [156]; inhibitory effect on nitric oxide production in LPS-activated RAW264.7 cells [157] Antinociceptive [158] Some anticomplementary activity [159] Neuroprotective [160,161]; promotion of proliferation of neural stem cells [162]; Inhibition of acetylcholinesterase [163] Inhibition of cancer cell proliferation [164], induction of apoptosis [165], antiproliferative [166], cytotoxic [167]; antiproliferative activity against HL-60, K562, HepG2 and CNE-1 cell lines [168] inhibition of MDA-MB-231 cancer cell migration [169]; Ability to activate PPARγ and PPARβ [170] Inhibition of α-glucosidase [171] Antibacterial effect against E. coli [172], against H. pylori and S. aureus [173]; against E. coli and S. aureus [174]; against Bacillus subtilis and S. aureus [175] Inhibitory effect on reverse transcriptase [176] Suppression of HCl/ethanol-induced gastric lesions [177] Inhibitory activity against osteoclast differentiation by suppressing TRAP activity in RANKL-induced RAW 264.7 macrophage cells [178] GlcO Daucosterol (29) Z. clinopodioides [44] Immunoregulatory effect [150] Anti-inflammatory activity [151]; anti-inflammatory in ear edema assay [152]; topical anti-inflammatory activity in the mouse ear edema model …”

Section: Triterpenes and Steroidsmentioning

confidence: 99%

Kazakh Ziziphora Species as Sources of Bioactive Substances

et al. 2016

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Ziziphora species represent the prototypical example of the Lamiaceae family. The phytochemicals present in Ziziphora include monoterpenic essential oils, triterpenes and phenolic substances belonging to the flavonoids. In Kazakh traditional medicine, Ziziphora species possess several medicinal uses. In particular, Z. bungeana Lam. and Z. clinopodioides Lam. are used for the treatment of illnesses related to the cardiovascular system or to combat different infections. Unfortunately, the majority of the information about the complex Ziziphora species is only available in Russian and Chinese language, therefore, we decided gather all available information on Kazakhstan Ziziphora, namely its content compounds, medicinal uses and published patents, to draw the attention of scientists to this very interesting plant with high medicinal potential.

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Immunoregulatory activity by daucosterol, a β-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice

Cited by 111 publications

References 23 publications

TLR2 Activation Is Essential to Induce a Th1 Shift in Human Peripheral Blood Mononuclear Cells by Plant Stanols and Plant Sterols

TLR2 Activation Is Essential to Induce a Th1 Shift in Human Peripheral Blood Mononuclear Cells by Plant Stanols and Plant Sterols

Suppressive Actions of Astragali Radix (AR) Ethanol Extract and Isolated Astragaloside I on HCl/ethanol-Induced Gastric Lesions

Kazakh Ziziphora Species as Sources of Bioactive Substances

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