Immunoreactivity between venoms and commercial antiserums in four Chinese snakes and venom identification by species-specific antibody

Gao, Jian-Fang; Wang, Jin; Qu, Yan‐Fu; Ma, Xiaojing; Ji, Xiang

doi:10.1016/j.jim.2012.10.016

Cited by 23 publications

(13 citation statements)

References 26 publications

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“…Current efforts in the diagnosis of snake envenomation have also been mainly focused on the development of antibody-based immunoassays. Snake venoms are highly complex mixtures that tend to have many common antigens among snakes from the same or even different genera [8], [9]. Direct use of crude antibody in immunoassays may have low specificity in species detection of snakebites.…”

Section: Introductionmentioning

confidence: 99%

“…Direct use of crude antibody in immunoassays may have low specificity in species detection of snakebites. Monoclonal antibodies against a single venom component or species-specific polyclonal antibodies produced by passing the crude antisera through the medium coupled with heterologous venoms were used to reduce cross reactivities [8], [10], [11].…”

Section: Introductionmentioning

confidence: 99%

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Recognition of Bungarus multicinctus Venom by a DNA Aptamer against β-Bungarotoxin

Zheng²,

Wang³

et al. 2014

PLoS ONE

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Antibody-based technology is the main method for diagnosis and treatment of snake bite envenoming currently. However, the development of an antibody, polyclonal or monoclonal, is a complicated and costly procedure. Aptamers are single stranded oligonucleotides that recognize specific targets such as proteins and have shown great potential over the years as diagnostic and therapeutic agents. In contrast to antibodies, aptamers can be selected in vitro without immunization of animals, and synthesized chemically with extreme accuracy, low cost and high degree of purity. In this study we firstly report on the identification of DNA aptamers that bind to β-bungarotoxin (β-BuTx), a neurotoxin from the venom of Bungarus multicinctus. A plate-SELEX method was used for the selection of β-BuTx specific aptamers. After 10 rounds of selection, four aptamer candidates were obtained, with the dissociation constant ranged from 65.9 nM to 995 nM measured by fluorescence spectroscopy. Competitive binding assays using both the fluorescently labeled and unlabeled aptamers revealed that the four aptamers bound to the same binding site of β-BuTx. The best binder, βB-1, bound specifically to β-BuTx, but not to BSA, casein or α-Bungarotoxin. Moreover, electrophoretic mobility shift assay and enzyme-linked aptamer assay demonstrated that βB-1 could discriminate B. multicinctus venom from other snake venoms tested. The results suggest that aptamer βB-1 can serve as a useful tool for the design and development of drugs and diagnostic tests for β-BuTx poisoning and B. multicinctus bites.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recognition of Bungarus multicinctus Venom by a DNA Aptamer against β-Bungarotoxin

Zheng²,

Wang³

et al. 2014

PLoS ONE

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“…В настоящее время в мировой практике идентификацию змеиных ядов проводят с помощью методов иммунологического анализа [17][18][19][20], массспектрометрии [9,11,[21][22][23][24], электрофореза [10,22,25,26] или сочетания этих методов (табл. 2).…”

Section: идентификацияunclassified

“…Поэтому можно ожидать, что для совершенствования нормативной документации на препараты, содержащие змеиный яд, будут разрабатываться варианты иммунологических методов, например, иммуноферментный. Так, разработка чувствительных и специфичных тест-систем для определения ядов в биологических жидкостях, как описано в исследованиях [17,18], могла бы решить вопрос не только диагностики отравлений, но и определения подлинности ядов в лекарственных препаратах.…”

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Drugs Containing Snake Venom: History of Development, Nomenclature, Identification

Григорьева¹,

Гунар²,

Сахно³

et al. 2018

Vedomosti Nauchnogo tsentra ekspertizy sredstv meditsinskogo pr

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“…The presence of certain antigenic components in snake venoms appear to be common in several snake species, genera and families (Marshall and Hermann, 1984;Russell, 1988) and antivenoms cross-reacted along them (Dong et al, 2003;Wahby and Ibrahim, 2008;Gao et al, 2013). Consequently, species-diagnosis has best been achieved with affinitypurified species-specific antibodies.…”

Section: Introductionmentioning

confidence: 99%

Antigenic cross-reactivity and species-specific identification of Pseudocerastes persicus fieldi snake venom

Ibrahim

El-Kady

2016

Toxicon

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Immunoreactivity between venoms and commercial antiserums in four Chinese snakes and venom identification by species-specific antibody

Cited by 23 publications

References 26 publications

Recognition of Bungarus multicinctus Venom by a DNA Aptamer against β-Bungarotoxin

Recognition of Bungarus multicinctus Venom by a DNA Aptamer against β-Bungarotoxin

Drugs Containing Snake Venom: History of Development, Nomenclature, Identification

Antigenic cross-reactivity and species-specific identification of Pseudocerastes persicus fieldi snake venom

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