Immunoreactive inhibin-like material in serum and gastric juice of patients with benign and malignant diseases of the stomach

Abstract

Summary Immunoreactive inhibin-like material (ILM) was measured by radioimmunoassay (RIA) in serum and gastric juice samples of 23 fasting normal men, 27 men with chronic superficial gastritis (CSG), and 21 men with carcinoma of stomach (5 for gastric analysis). Serum ILM levels in carcinoma of stomach patients (367+55.5ngml-1) were significantly higher than in normal men (15.4+2.6ngml-1; P<0.01) and in patients with CSG (109.8 + 17.7 ng ml-1; P<0.05). Sixty two per cent and 86% of patients with carcinoma of stomach showed elevated ILM levels which were higher than the highest noted in patients with CSG and normal men respectively.

Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: Comparison of its distribution in normal, benign and malignant prostates

“…Antisera Rabbit antihuman seminal plasma ILM Specific rabbit antiserum raised by active immunization in this laboratory with the homogeneous preparation of ILM from seminal plasma, was used for immuno-staining of tissue ILM. Antiserum was highly specific for ILM by the criterion that it did not show cross reactivity with 23 peptides which included protein hormones of pituitary and placental origin, foetal and gastrointestinal peptides and a few other peptides (Shanbaugh et al, 1985). The antiserum was capable of binding 50% of radio-iodinated ILM at a dilution of 1: 10,000.…”

“…The antiserum was capable of binding 50% of radio-iodinated ILM at a dilution of 1: 10,000. From a Scatchard analysis the affinity constant Ka of ILM antiserum was calculated as 2.06 x 1010 M. The details of the antiserum were reported earlier (Shanbaugh et al, 1984(Shanbaugh et al, , 1985.…”

Age-Related Changes in Human Prostatic Inhibin Peptide: An Immunocytochemical Study

“…PIP raised in our laboratory [9], using homogeneous preparation of PIP from seminal plasma, served as the first antiserum. For control, normal rabbit serum (NRS) was substituted for the first antiserum.…”

Hormonal modulation of Invitro Biosynthesis of Inhibin like Peptide by Human Prostate: Hormonelle Modulation der In-vitro-Biosynthese des Inhibin-ähnlichen Peptids durch die menschliche Prostata

“…The total amount of hSPI present in the medium was calculated from this ratio. A specific and sensitive radioimmunoassay as described earlier was used to estimate inhibin content in the medium samples obtained during various experiments (Shanbaugh et al -1985). The suitability of the assay was established on the basis of parallel dose response curve with hSPI standard and culture medium samples.…”