1989
DOI: 10.1159/000125282
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Immunoreactive Delta Sleep-Inducing Peptide Secretion from Mouse Dissociated, Anterior Pituitary Cells: Regulation by Corticotropin-Releasing Factor and Arginine Vasopressin

Abstract: Immunoreactive delta sleep-inducing peptide (IR-DSIP) has previously been localized to the ACTH/MSH cells of the human and porcine pituitary gland. In the present report, the distribution of IR-DSIP in the mouse pituitary gland was examined by immunocytochemistry. In this species, IR-DSIP was found to be co-localized with thyroid-stimulating hormone (TSH) in anterior pituitary thyrotrophs and was also present in nerve fibers in the posterior and intermediate lobes. The effect of synthetic DSIP on IR-ACTH relea… Show more

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Cited by 17 publications
(16 citation statements)
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“…a nonapeptide which has been found to have sleep-inducing and -main taining properties in several species, including humans [10][11][12][13], on the sleep parameters of patients with Cushing syndrome and normal volunteers. DSIP-like immunoreactivity (DSIP-LI) has been found in thyrotropic cells in mice [14] and corticotropic/melanotropic cells in humans and pigs [15,16], where it appeared to be colocalized with ACTH and corticotropin-like intermediate-lobe peptide (CLIP). We, therefore, hypothesized that levels of this hormone may be altered in patients with Cushing syn drome and may be related to their sleep disturbances.…”
Section: Introductionmentioning
confidence: 99%
“…a nonapeptide which has been found to have sleep-inducing and -main taining properties in several species, including humans [10][11][12][13], on the sleep parameters of patients with Cushing syndrome and normal volunteers. DSIP-like immunoreactivity (DSIP-LI) has been found in thyrotropic cells in mice [14] and corticotropic/melanotropic cells in humans and pigs [15,16], where it appeared to be colocalized with ACTH and corticotropin-like intermediate-lobe peptide (CLIP). We, therefore, hypothesized that levels of this hormone may be altered in patients with Cushing syn drome and may be related to their sleep disturbances.…”
Section: Introductionmentioning
confidence: 99%
“…The immunoprecipitates were washed twice in the immunoprecipitation buffer and extracted from the Sepharose beads by suspending in the SDSjPAGE treatment buffer (described below) and analyzed by SDS/PAGE. The immunoprecipitations were carried out with two previously described antisera against synthetic DSIP: one (code 7915; generated by Dr R. Ekman, Lund University) is a rabbit antiserum against DSIP coupled to bovine serum albumin and recognizes the midportion of synthetic rabbit DSIP [20]; the other (code R:5; a kind gift from Dr P. G. Vallet, Geneva, Switzerland) was raised in a rat against thyroglobulin-conjugated DSIP [I 5, 161. For control experiments, increasing concentrations (0.2 -1.25 mg/ml) of synthetic DSIP (Peninsula Labs, Belmont, CA, USA) was added to the incubation for preabsorption of the DSIP antibodies.…”
Section: Inimunoprecipitationmentioning
confidence: 99%
“…'s 7911 (Milab AB, Malmo, Sweden) and 7915. Both antisera have been characterized previously [20,231 and were used at a dilution of 1 :500000 and 1 :60000, respectively. Antiserum 791 1 recognizes mainly the N-terminus of DSIP while 791 5 is more specific for the midportion of the molecule.…”
Section: Ria For Dsipmentioning
confidence: 99%
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