Immunoprotective inference of experimental chronic Trichinella spiralis infection on house dust mites induced allergic airway remodeling

Elmehy, Dalia A.; Elkholy, Rasha A; Elkelany, Maram; Tahoon, Dina; Elkholy, Reem; Soliman, Nema A.; Saad, Marwa A. Hasby; El-Ebiary, Ahmad A.; Gamea, Ghada A.

doi:10.1016/j.actatropica.2021.105934

Cited by 7 publications

(6 citation statements)

References 58 publications

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“…From this point of view, it would not be possible to study the modulation, which has already been described, between the Th2 and Treg responses. Despite the data from our group, the studies were carried out by different investigators in different experimental models (38,39). This reinforces our results, since of imbalance between Th2/Treg immune response represented by high levels of IL-10 in the control or Basal group, and low levels in animals in the sick group, was repeated.…”

Section: Discussionsupporting

confidence: 83%

Photobiomodulation Therapy Restores IL-10 Secretion in a Murine Model of Chronic Asthma: Relevance to the Population of CD4+CD25+Foxp3+ Cells in Lung

Brito¹,

Herculano²,

Estefano-Alves³

et al. 2022

Front. Immunol.

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It is largely known that photobiomodulation (PBM) has beneficial effects on allergic pulmonary inflammation. Our previous study showed an anti-inflammatory effect of the PBM in an acute experimental model of asthma, and we see that this mechanism is partly dependent on IL-10. However, it remains unclear whether the activation of regulatory T cells is mediated by PBM in a chronic experimental model of asthma. In this sense, the objective of this study was to verify the anti-inflammatory role of the PBM in the pulmonary inflammatory response in a chronic experimental asthma model. The protocol used for asthma induction was the administration of OVA subcutaneously (days 0 and 14) and intranasally (3 times/week, for 5 weeks). On day 50, the animals were sacrificed for the evaluation of the different parameters. The PBM used was the diode, with a wavelength of 660 nm, a power of 100 mW, and 5 J for 50 s/point, in three different application points. Our results showed that PBM decreases macrophages, neutrophils, and lymphocytes in the bronchoalveolar lavage fluid (BALF). Moreover, PBM decreased the release of cytokines by the lung, mucus, and collagen in the airways and pulmonary mechanics. When we analyzed the percentage of Treg cells in the group irradiated with laser, we verified an increase in these cells, as well as the release of IL-10 in the BALF. Therefore, we conclude that the use of PBM therapy in chronic airway inflammation attenuated the inflammatory process, as well as the pulmonary functional and structural parameters, probably due to an increase in Treg cells.

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Section: Discussionsupporting

confidence: 83%

Photobiomodulation Therapy Restores IL-10 Secretion in a Murine Model of Chronic Asthma: Relevance to the Population of CD4+CD25+Foxp3+ Cells in Lung

Brito¹,

Herculano²,

Estefano-Alves³

et al. 2022

Front. Immunol.

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“…Chronic infection with T . spiralis can mediate protection against allergic asthma by eliciting a regulatory T cell population and increasing IL-10 levels [ 23 ]. Evidence also indicates that T .…”

Section: Discussionmentioning

confidence: 99%

Preexisting Trichinella spiralis infection attenuates the severity of Pseudomonas aeruginosa-induced pneumonia

et al. 2022

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Background A range of helminth species involve the migration of developing larvae through the lung and establish chronic infections in the host that include potent immune regulatory effects. Trichinella spiralis is one of the most successful parasitic symbiotes. After released by intestinal female adult worms, newborn larvae of T. spiralis travel through the circulatory system to the lung and finally reach skeletal muscle cells. As unique inflammation modulator of intracellular parasitism, T. spiralis shows improved responses to autoimmune disease and viral pulmonary inflammation by exerting immunomodulatory effects on innate and adaptive immune cells. Methodology/Principal findings C57BL/6 mice were divided into four groups: uninfected; helminth- T. spiralis infected; P. aeruginosa infected; and co-infected. Mice infected with T. spiralis were incubated for 6 weeks, followed by P. aeruginosa intranasal inoculation. Bronchial alveolar lavage fluid, blood and lung samples were analyzed. We found that T. spiralis induced Th2 response in the mouse lung tissue, increased lung CD4+ T cells, GATA3, IL-4, IL-5 and IL-13 expression. Pre-existing T. spiralis infection decreased lung neutrophil recruitment, inflammatory mediator IL-1β and IL-6 expression and chemokine CXCL1 and CXCL2 release during P. aeruginosa- pneumonia. Furthermore, T. spiralis co-infected mice exhibited significantly more eosinophils at 6 hours following P. aeruginosa infection, ameliorated pulmonary inflammation and improved survival in P. aeruginosa pneumonia. Conclusions These findings indicate that a prior infection with T. spiralis ameliorates experimental pulmonary inflammation and improves survival in P. aeruginosa pneumonia through a Th2-type response with eosinophils.

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“…Moreover, according to Bruschi, Ashour and Othman [20], T. spiralis has an inhibitory effect on neutrophil inflammatory function, experimental autoimmune encephalomyelitis and regulated human dendritic cell activation. Moreover, T. spiralis infection protected mice against colitis [36] and acute lung injury induced by bacterial lipopolysaccharide (LPS) [37], as well as their immunoprotective inference on house dust mites–induced allergic airway remodelling in mice [38].…”

Section: Discussionmentioning

confidence: 99%

Trichinella spiralis Larval Extract as a Biological Anti‐Tumor Therapy in a Murine Model of Ehrlich Solid Carcinoma

Younis,

Salama,

Elmehy

et al. 2024

Parasite Immunology

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Trichinella spiralis (T. spiralis) is an immunomodulating parasite that can adversely affect tumor growth and extend host lifespan. The aim of this study was to elucidate the mechanisms by which T. spiralis larval antigens achieve this effect using Ehrlich solid carcinoma (ESC) murine model. Assessment was done by histopathological and immunohistochemical analysis of caspase‐3, TNF‐α, Ki‐67 and CD31. Additionally, Bcl2 and Bcl2‐associated protein X (Bax) relative gene expression was assessed by molecular analysis for studying the effect of T. spiralis crude larval extract (CLE) antigen on tumor necrosis, apoptosis, cell proliferation and angiogenesis. We found that both T. spiralis infection and CLE caused a decrease in the areas of necrosis in ESC. Moreover, they led to increased apoptosis through activation of caspase‐3, up‐regulation of pro‐apoptotic gene, Bax and down‐regulation of anti‐apoptotic gene, Bcl2. Also, T. spiralis infection and CLE diminished ESC proliferation, as evidenced by decreasing Ki‐67. T. spiralis infection and CLE were able to suppress the development of ESC by inhibiting tumor proliferation, inducing apoptosis and decreasing tumor necrosis, with subsequent decrease in tumor metastasis. T. spiralis CLE antigen may be considered as a promising complementary immunotherapeutic agent in the treatment of cancer.

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Immunoprotective inference of experimental chronic Trichinella spiralis infection on house dust mites induced allergic airway remodeling

Cited by 7 publications

References 58 publications

Photobiomodulation Therapy Restores IL-10 Secretion in a Murine Model of Chronic Asthma: Relevance to the Population of CD4+CD25+Foxp3+ Cells in Lung

Photobiomodulation Therapy Restores IL-10 Secretion in a Murine Model of Chronic Asthma: Relevance to the Population of CD4+CD25+Foxp3+ Cells in Lung

Preexisting Trichinella spiralis infection attenuates the severity of Pseudomonas aeruginosa-induced pneumonia

Trichinella spiralis Larval Extract as a Biological Anti‐Tumor Therapy in a Murine Model of Ehrlich Solid Carcinoma

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