Immunoprotective activities of a Streptococcus suis pilus subunit in murine models of infection

Another turn of the screw in shaving Gram-positive bacteria: Optimization of proteomics surface protein identification in Streptococcus pneumoniae

“…proteases has been shown to be a valuable tool for identifying quickly a set of some tens of proteins (the "surfome") whose most frequent application is the discovery of vaccine candidates [9,11,28,29] or virulence factors [30]. However, numerous studies have shown that cytoplasmic contamination, even at a relatively low degree, is unavoidable.…”

Streptococcus suisinfection

“…In particular note, all the three newly-identified immunogenic antigens (6PGD, HP0197, and HP245) are elucidated by the same Chen’s research group in China (Table 3). Interestingly, proteomics also facilitated to discover three new immunogenic proteins SsPepO, 173 Sat (HP0272), 112 , 174 and the pilus subunit PAPI-2b 125 (Table 3). In disagreement with their former observation, 121 Li and coworkers 122 demonstrated that SAO surface antigen can confer efficient protection in both mice and piglets against virulent SS2 infection (Table 3).…”

Genomic organization, structure, regulation and pathogenic role of pilus constituents in major pathogenic Streptococci and Enterococci

“…More recently, 4 gene clusters encoding putative pilus-like structures were identified in S. suis, designated srtBCD and srtF, srtE and srtG, according to the type of class C sortases present in the respective locus (Fittipaldi et al, 2007;Rodriguez-Ortega et al, 2008;Takamatsu et al, 2009). The most prevalent cluster, srtF, was recently studied in detail by Fittipaldi et al (2010) and Garibaldi et al (2010). For many other species, evidence for pilus gene clusters is exclusively based on bioinformatic evidence (Plyusnin et al, 2009).…”