Immunoprotection of recombinant Eg.P29 against Echinococcus granulosus in sheep

Wang, Hao; Li, Zihua; Gao, Fei; Zhao, Jiaqing; Zhu, Mingzhao; He, Xin; Niu, Nan; Zhao, Wei

doi:10.1007/s11259-016-9656-7

Cited by 31 publications

(31 citation statements)

References 30 publications

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“…Previous studies have shown that rEg.P29 can induce protective immunity in mice against secondary infection of echinococcus granulosus. It has also been shown that cellular immunity is further polarized into Th1 after vaccination of rEg.P29 [6,13]. So far, the core biological function of rEg.P29 in echinococcus biology is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Eg.P29 gene was taken from hydatid cysts of patients in the General Hospital of Ningxia Medical University (the Chinese strain of the gene was recorded into GeneBank: sequence number AF078931) [9]. Plasmid Eg.P29/pET28a was previously constructed and expressed in Escherichia coli at our laboratory [13,14]. The purified rEg.P29 was identified with 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot [8].…”

Section: Methodsmentioning

confidence: 99%

“…Prior studies suggested a possible role of rEg.P29 as a developmentally regulated component of the E. granulosus metacestode [6]. Our previous study confirmed that recombinant Eg.P29 (rEgP29) could protect against echinococcus granulosus secondary infection in sheep and mice [13,14]. Immunization with rEg.P29 induced protective immunity against sheep carrying natural infection of E. granulosus eggs [5].…”

Section: Introductionmentioning

confidence: 99%

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MiR-374b-5p Regulates T Cell Differentiation and Is Associated with rEg.P29 Immunity

Zhu

et al. 2020

BioMed Research International

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Cystic echinococcosis (CE) is a zoonotic disease caused by Echinococcus granulosus (Eg) infection. Our previous study confirmed that recombinant Eg.P29 (rEg.P29) could protect against echinococcus granulosus secondary infection in sheep and mice. The aim of the study was to investigate the association between immunoprotection of rEg.P29 vaccine and mmu-miR-374b-5p (miR-374b-5p) and study the immunity influence of miR-374b-5p on CD4+ T cells in mice spleen. MiR-374b-5p level was significantly increased after the second-week and the fourth week of vaccination with rEg.P29. Overexpression of miR-374b-5p increased IFN-γ, IL-2, IL-17A mRNA levels and decreased IL-10 mRNA levels in CD4+ T cells. Moreover, the inhibition of miR-374b-5p decreased IFN-γ and IL-17A and increased IL-10 mRNA levels in CD4+ T cells; this was further confirmed by the flow cytometry. The vaccination of rEg.P29 enhanced miR-374b-5p expression that was associated with a higher Th1 and Th17 immune response, a lower IL-10 mRNA production with miR-374b-5p overexpression, a lower Th1 immune response, and a higher IL-10 mRNA levels with miR-374b-5p inhibitions. To sum up, these data suggest that miR-374b-5p may participate in rEg.P29 immunity by regulating Th1 and Th17 differentiation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MiR-374b-5p Regulates T Cell Differentiation and Is Associated with rEg.P29 Immunity

Zhu

et al. 2020

BioMed Research International

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“…corti endophilin is considerably similar to Echinococcus spp. P29 proteins, which were shown to be highly efficient host protective antigens when used as vaccines [ 51 , 52 ]. A similar role for M .…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions

et al. 2016

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Accumulating evidences have assigned a central role to parasite-derived proteins in immunomodulation. Here, we report on the proteomic identification and characterization of immunomodulatory excretory-secretory (ES) products from the metacestode larva (tetrathyridium) of the tapeworm Mesocestoides corti (syn. M. vogae). We demonstrate that ES products but not larval homogenates inhibit the stimuli-driven release of the pro-inflammatory, Th1-inducing cytokine IL-12p70 by murine bone marrow-derived dendritic cells (BMDCs). Within the ES fraction, we biochemically narrowed down the immunosuppressive activity to glycoproteins since active components were lipid-free, but sensitive to heat- and carbohydrate-treatment. Finally, using bioassay-guided chromatographic analyses assisted by comparative proteomics of active and inactive fractions of the ES products, we defined a comprehensive list of candidate proteins released by M. corti tetrathyridia as potential suppressors of DC functions. Our study provides a comprehensive library of somatic and ES products and highlight some candidate parasite factors that might drive the subversion of DC functions to facilitate the persistence of M. corti tetrathyridia in their hosts.

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“…A vaccine to protect herbivorous livestock against CE can be effective in reducing economic costs and increasing of livestock products [ 8 ]. The EG95 vaccine against CE has proven to be highly effective [ 9 ]. Furthermore, it is established that antibody has an important role against infection with the eggs of E. granulosus in sheep and rodents [ 10 - 14 ].…”

Section: Introductionmentioning

confidence: 99%

Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens

Valizadeh¹,

Behzad²,

Badirzadeh³

et al. 2017

Vet World

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Aim:Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine.Materials and Methods:Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 µg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.Results:After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).Conclusion:The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.

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Immunoprotection of recombinant Eg.P29 against Echinococcus granulosus in sheep

Cited by 31 publications

References 30 publications

MiR-374b-5p Regulates T Cell Differentiation and Is Associated with rEg.P29 Immunity

MiR-374b-5p Regulates T Cell Differentiation and Is Associated with rEg.P29 Immunity

Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions

Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens

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