Immunization of sheep against Echinococcus granulosus with protoscolex tegumental surface antigens

Abstract

Aim:Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine.Materials and Methods:Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 µg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.Results:After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).Conclusion:The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.

Customizing the Protoscolicidal Activity by a Drug Delivery System: Application of Guar Gum in Electrospun Nanofibers

“…The livers infected with hydatid cyst of slaughtered domestic livestock were prepared from the industrial slaughterhouse of Arak city - Markazi province ( 8 ) ( Fig. 4 ).…”

“…Human infection with parasites has been known as one of the health problems of the world from the very distant past. Currently, surgery is a selective treatment for single-cavity hydatid cysts ( 4 – 8 ). However, several patients cannot be candidates for surgery due to multiple lesions in different organs or due to specific physical conditions.…”

Use of Veterinary Vaccines for Livestock as a Strategy to Control Foodborne Parasitic Diseases

“…Despite EG95 vaccine has demonstrated to avoid infection in sheep after three doses during the first year of life suggesting that it would be a practical vaccine for implementation by livestock farmers; it is necessary to analyze different vaccination schedules and determine the effect on the reduction of infection in dogs and humans. In addition, the EG95 vaccine is very expensive, which limits its application to animals reared only in some of the most developed countries (Valizadeh et al, 2017 ). As an alternative vaccination strategy, a number of important antigen vaccine candidates targeted against the definitive hosts have been evaluated in dogs, including a 66-kDa fibrous protein called EgA31, which plays a key role in infection of humans and dogs (Petavy et al, 2008 ); a fatty acid-binding protein from E. granulosus called E. granulosus differential factor 1 (EgDf1), involved in the uptake, storage and transport of fatty acids in the parasite (Chabalgoity et al, 2000 ); and three proteins from the family of E. granulosus EgM, called EgM123, EgM4, and EgM9, potentially involved in egg maturation (Zhang et al, 2006 ; Zhang Z.…”

Traumatic rupture of liver hydatid cysts into the peritoneal cavity of an 11-year-old boy: a case report from Iran

“…Hydatid cyst (HC) disease, a zoonotic parasitic infection, occurs during the larval stage of a cestode named Echinococcus granulosus (E. granulosus) 1 . The disease is endemic and hyper-endemic in pastoral regions of the world 2,3 . Although HC may develop in any organ, it generally occurs in the liver (50-75%) and lungs (18-30%), and is characterized by a cystic form of lesions 4 .…”