Immunoprophylactic evaluation of recombinant gametocyte 22 antigen of Eimeria tenella in broiler chickens

Rafiqi, Shafiya Imtiaz; Garg, Rajat; Ram, Hira; Reena, K.; Asari, Mayurkumar; Kumari, Priyanka; Kundave, V R; Singh, Mithilesh; Banerjee, P. S.

doi:10.1007/s00436-018-06198-2

Cited by 12 publications

(9 citation statements)

References 34 publications

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“…In recent years, a number of E. tenella antigens have been tested as candidate antigens for subunit or DNA vaccines, such as surface antigen 16 (SAG16), SAG22, gametocyte 22 antigen, and silent information regulator 2 [ 9 , 14 , 15 ]. Currently, the protective efficacy of several proteins has been not evaluated, such as EtROP17.…”

Section: Introductionmentioning

confidence: 99%

Identification and Protective Efficacy of Eimeria tenella Rhoptry Kinase Family Protein 17

Liu

Zheng

et al. 2022

Animals

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Eimeria tenella encodes a genome of approximately 8000 genes. To date, however, very few data are available regarding E. tenella rhoptry kinase family proteins. In the present study, the gene fragment encoding the mature peptide of the rhoptry kinase family protein 17 of E. tenella (EtROP17) was amplified by PCR and expressed in E. coli. Then, we generated polyclonal antibodies that recognize EtROP17 and investigated the expression of EtROP17 in the merozoite stage of E. tenella by immunofluorescent staining and Western blot analysis. Meanwhile, the protective efficacy of rEtROP17 against E. tenella was evaluated in chickens. Sequencing analysis showed that a single base difference at sequence position 1901 was observed between the SD-01 strain and the Houghton strain. EtROP17 was expressed in the merozoite stage of E. tenella. The results of the animal challenge experiments demonstrated that vaccination with rEtROP17 significantly reduced cecal lesions and oocyst outputs compared with the challenged control group. Our findings indicate that EtROP17 could serve as a potential candidate for developing a new vaccine against E. tenella.

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Section: Introductionmentioning

confidence: 99%

Identification and Protective Efficacy of Eimeria tenella Rhoptry Kinase Family Protein 17

Liu

Zheng

et al. 2022

Animals

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“…Proteases break down GAM proteins into tyrosine-rich peptides, which are then oxidatively crosslinked by peroxidases and incorporated into the oocyst wall in dityrosine bonds [16,17]. GAM proteins are the most studied proteins of the oocyst wall; they were developed as antigens for transmission blocking vaccines targeting the gametocyte-specific proteins GAM56, GAM82 and GAM22 of Eimeria [13,[18][19][20]. Cysteine-rich OWPs have also been described in Toxoplasma gondii [21].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Cystoisospora suis sexual stages in vitro

et al. 2020

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Background: The porcine coccidium Cystoisospora suis is characterized by a complex life-cycle during which asexual multiplication is followed by sexual development with two morphologically distinct cell types, the micro-and macrogametes. Genes related to the sexual stages and cell cycle progression were previously identified in related Apicomplexa. Dynein light chain type 1 and male gamete fusion factor HAP2 are restricted to microgametes. Tyrosine-rich proteins and oocyst wall proteins are a part of the oocyst wall. The Rad51/Dmc1-like protein and Nima-related protein kinases are associated with the cell cycle and fertilization process. Here, the sexual stages of C. suis were characterized in vitro morphologically and for temporal expression changes of the mentioned genes to gain insight into this poorly known phase of coccidian development. Methods: Sexual stages of C. suis developing in vitro in porcine intestinal epithelial cells were examined by light and electron microscopy. The transcriptional levels of genes related to merozoite multiplication and sexual development were evaluated by quantitative real-time PCR at different time points of cultivation. Transcription levels were compared for parasites in culture supernatants at 6-9 days of cultivation (doc) and intracellular parasites at 6-15 doc. Results: Sexual stage of C. suis was detected during 8-11 doc in vitro. Microgamonts (16.8 ± 0.9 µm) and macrogamonts (16.6 ± 1.1 µm) are very similar in shape and size. Microgametes had a round body (3.5 ± 0.5 µm) and two flagella (11.2 ± 0.5 µm). Macrogametes were spherical with a diameter of 12.1 ± 0.5 µm. Merozoite gene transcription peaked on 10 doc and then declined. Genes related to the sexual stages and cell cycle showed an upregulation with a peak on 13 doc, after which they declined. Conclusions: The present study linked gene expression changes to the detailed morphological description of C. suis sexual development in vitro, including fertilization, meiosis and oocyst formation in this unique model for coccidian parasites. Following this process at the cellular and molecular level will elucidate details on potential bottlenecks of C. suis development (applicable for coccidian parasites in general) which could be exploited as a novel target for control.

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“…The most recent study using Gam proteins involve the recombinant rEtGam22. Vaccination of chicken with EtGam22 significantly elicited both Th1 and Th2 cytokine mediated immune responses and induced protection against E. tenella and E. maxima-infected chickens [129]. Taken together, these studies indicate that Gam proteins are potent immunogens for the use as vaccines against chicken coccidiosis, as they induce a diverse and robust immunity [134].…”

Section: Breaking the Cycle-targeting Sexual Stages Of Apicomplexan Parasites For Interventionmentioning

confidence: 79%

“…The production of this vaccine based on affinity purification of native gametocyte proteins from parasites, however, was expensive and time-consuming. Since then, several proteins (molecular weights of 14, 22, 30, 56, 82 and 230 kDa) associated with E. maxima, E. tenella, and E. necatrix gametocytes have been proposed as potential vaccine targets to induce immunity [128][129][130][131][132][133]. The most recent study using Gam proteins involve the recombinant rEtGam22.…”

Section: Breaking the Cycle-targeting Sexual Stages Of Apicomplexan Parasites For Interventionmentioning

confidence: 99%

Sexual Development in Non-Human Parasitic Apicomplexa: Just Biology or Targets for Control?

Cruz‐Bustos

Feix

Ruttkowski

et al. 2021

Animals

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The phylum Apicomplexa is a major group of protozoan parasites including gregarines, coccidia, haemogregarines, haemosporidia and piroplasms, with more than 6000 named species. Three of these subgroups, the coccidia, hemosporidia, and piroplasms, contain parasites that cause important diseases of humans and animals worldwide. All of them have complex life cycles involving a switch between asexual and sexual reproduction, which is key to their development. Fertilization (i.e., fusion of female and male cells) results in the formation of a zygote that undergoes meiosis, forming a new generation of asexual stages. In eukaryotes, sexual reproduction is the predominant mode of recombination and segregation of DNA. Sex is well documented in many protist groups, and together with meiosis, is frequently linked with transmission to new hosts. Apicomplexan sexual stages constitute a bottleneck in the life cycle of these parasites, as they are obligatory for the development of new transmissible stages. Consequently, the sexual stages represent attractive targets for vaccination. Detailed understanding of apicomplexan sexual biology will pave the way for the design and implementation of effective transmission-blocking strategies for parasite control. This article reviews the current knowledge on the sexual development of Apicomplexa and the progress in transmission-blocking vaccines for their control, their advantages and limitations and outstanding questions for the future.

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Immunoprophylactic evaluation of recombinant gametocyte 22 antigen of Eimeria tenella in broiler chickens

Cited by 12 publications

References 34 publications

Identification and Protective Efficacy of Eimeria tenella Rhoptry Kinase Family Protein 17

Identification and Protective Efficacy of Eimeria tenella Rhoptry Kinase Family Protein 17

Characterization of Cystoisospora suis sexual stages in vitro

Sexual Development in Non-Human Parasitic Apicomplexa: Just Biology or Targets for Control?

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