Immunopotentiation and antitumor effects of a ginsenoside Rg3-fortified red ginseng preparation in mice bearing H460 lung cancer cells

Park, Dongsun; Bae, Dae-Kwon; Jeon, Jeong Hee; Lee, Jin Soo; Oh, Namgil; Yang, Goeun; Yang, Yaewon; Kim, Tae Kyun; Song, Jugyeong; Lee, Sun Hee; Song, Byeng Sub; Jeon, Tae Hawn; Kang, Shin Jyung; Joo, Seong Soo; Kim, Seung Up; Kim, Yun-Bae

doi:10.1016/j.etap.2011.01.008

Cited by 61 publications

(41 citation statements)

References 34 publications

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“…In Japan, persimmon juice has been traditionally used to treat hypertension and prevent heart attacks [8]. The hypotensive effect of Panax ginseng is attributable to the saponin, ginsenoside Rg3, which could be converted by acidic hydrolysis from protopanaxadiol-type saponins and exhibits protective effects against hypertension [9], [10], [11], [12], [13], [14]. Furthermore, arginine–fructose (Arg-Fru) produced from arginine–fructose–glucose (Arg-Fru-Glc) by the Maillard reaction under acidic condition is known to be a specific active compound in Korean Red Ginseng [15].…”

Section: Introductionmentioning

confidence: 99%

Antihypertensive effect of Korean Red Ginseng by enrichment of ginsenoside Rg3 and arginine–fructose

Lee

Bae

Park

et al. 2016

Journal of Ginseng Research

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BackgroundGinsenoside Rg3 and arginine–fructose (Arg-Fru) are known as the hypotensive compounds of Panax ginseng; however, their efficacy on antihypertension has not been reported yet to our best knowledge. Thus, hypotensive components-enriched fraction of red ginseng (HCEF-RG) was prepared from fine root concentrate (FR) and their antihypertensive effects were investigated in spontaneously hypertensive rats (SHR).MethodsMale SHRs were divided into six groups: control (Wistar Kyoto, SHR); FR 500; FR 1,000; HCEF-RG 500; and HCEF-RG 1,000; samples (mg/kg body weight) were orally administered every day for 8 wk. Blood pressure was monitored at 1 wk, 2 wk, 3 wk, 4 wk, 6 wk, and 8 wk by tail cuff method. At 8 wk after samples administration, mice were killed for the measurement of renin activity (RA), angiotensin-I converting enzyme inhibition, angiotensin II, and nitric oxide (NO) levels in plasma.ResultsHCEF-RG with four-fold more Rg3 and 24-fold more Arg-Fru contents was successfully prepared from reacted mixtures of FR and persimmon vinegar (12 times against FR, v/v) at 80°C for 18 h. Both FR 1,000 and HCEF-RG 1,000 showed lowered systolic blood pressure than SHR control group and HCEF-RG 1,000 group exhibited a significant decrease in diastolic blood pressure. RA was significantly lowered in all treated groups, while angiotensin II did not affect by FR and HCEF-RG treatment. However, angiotensin-I converting enzyme inhibition and NO in FR 1,000 and HCEF-RG 1,000 were significantly increased compared with SHR control group.ConclusionHCEF-RG is more effective and useful for alleviating hypertension than FR, implying the health benefit of Rg3 and Arg-Fru.

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Section: Introductionmentioning

confidence: 99%

Antihypertensive effect of Korean Red Ginseng by enrichment of ginsenoside Rg3 and arginine–fructose

Lee

Bae

Park

et al. 2016

Journal of Ginseng Research

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“…Positive control was carried out with doxorubicin [Table 1], a well known antitumor compound. [34] Nontumor cells 3T3, HF and Huvec were less affected by HE extract than B16F10-Nex2 cells [Figure 1]. …”

Section: Resultsmentioning

confidence: 99%

Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo

et al. 2014

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Background:Pyrostegia venusta (Ker. Gawl.) Miers (Bignoniacea) is a medicinal plant from the Brazilian Cerrado used to treat leucoderma and common diseases of the respiratory system.Objective:To investigate the antitumor activity of P.venusta extracts against melanoma.Materials and Methods:The cytotoxic activity and tumor induced cell death of heptane extract (HE) from P. venusta flowers was evaluated against murine melanoma B16F10-Nex2 cells in vitro and in a syngeneic model in vivo.Results:We found that HE induced apoptosis in melanoma cells by disruption of the mitochondrial membrane potential, induction of reactive oxygen species and late apoptosis evidenced by plasma membrane blebbing, cell shrinkage, chromatin condensation and DNA fragmentation, exposure of phosphatidylserine on the cell surface and activation of caspase-2,-3,-8,-9. HE was also protective against singeneyc subcutaneous melanoma HE compounds were also able to induce cell cycle arrest at G2/M phases on tumor cells. On fractionation of HE in silica gel we isolated a cytotoxic fraction that contained a mixture of saturated hydrocarbons identified by 1H NMR and GC-MS analyses. Predominant species were octacosane (C28H58-36%) and triacontane (C30H62-13%), which individually showed significant cytotoxic activity against murine melanoma B16F10-Nex2 cells in vitro and a very promising antitumor protection against subcutaneous melanoma in vivo.Conclusion:The results suggest that the components of the heptane extract, mainly octasane and triacontane, which showed antitumor properties in experimental melanoma upon regional administration, might also be therapeutic in human cancer, such as in the mostly epidermal and slowly invasive melanomas, such as acral lentiginous melanoma, as an adjuvant treatment to surgical excision.

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“…Consistently, production of NO and TNF-α was induced by Woongjin fermented red ginseng extract (WFRG) [17] in RAW 264.7 cells. In a lung tumor murine model, ginsenoside Rg3-fortified red ginseng (Rg3-RGP) induced production of NO from peritoneal macrophages in vivo [18] , thus Rg3-RGP reduced tumor size and improved the clinical outcome in cancer mouse models. Interestingly, peritoneal macrophages showed potential direct anti-tumor activity when ginseng polysaccharide (GPS)-pretreated macrophages were co-cultured with other cancer cell such as K562, HL-60, or KG1-α [19] .…”

Section: P Ginseng Potentiates Macrophage Functions Against Cancer Dmentioning

confidence: 99%

Panax ginseng as a potential modulator of macrophages

Nguyen¹,

Rhee

2016

Macrophage

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Bacteria-induced sepsis is a medical emergency requiring immediate intervention; the key to prevent sepsis is to prevent an infection from occurring in the first place. Although Panax ginseng (Korean ginseng) has been well known as an immune modulator, its role in bacterial sepsis is not well understood. Using RAW 264.7 macrophages and a murine model, we elucidated the protective effect of P. ginseng against community-associated Streptococcus pneumoniae (pneumococcus)-induced sepsis via inhibition of inflammation. In pneumococcal infections, pre-treatment with P. ginseng decreased the expressions of Toll-like receptor 4 (TLR4) and tumor necrosis factor (TNF)-ɑ in RAW 264.7 macrophages, but increased the expression of phosphoinositide 3-kinase (PI3K) and AKT kinase, thereby enhancing cellular viability. Furthermore, secretion of pro-inflammatory cytokines (TNF-ɑ and interleukin (IL)-1β) as well as neutrophil infiltration was diminished by pre-treatment with P. ginseng. As a result, mice pre-treated with P. ginseng demonstrated significantly higher survival rate and body weight gain than the non-treated mice (control). Treated mice showed diminished colonization of bacteria and morbidity. In addition, some studies have indicated that P. ginseng or ginseng compounds activated macrophage functions, which supports the immune system against cancer and other diseases. In the present study, we will discuss dual roles of P. ginseng in modulating macrophage functions.

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Immunopotentiation and antitumor effects of a ginsenoside Rg3-fortified red ginseng preparation in mice bearing H460 lung cancer cells

Cited by 61 publications

References 34 publications

Antihypertensive effect of Korean Red Ginseng by enrichment of ginsenoside Rg3 and arginine–fructose

Antihypertensive effect of Korean Red Ginseng by enrichment of ginsenoside Rg3 and arginine–fructose

Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo

Panax ginseng as a potential modulator of macrophages

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