Immunopotentiating Activity of Nigerooligosaccharides for the T Helper 1-Like Immune Response in Mice

Abstract

The immunopotentiating activity ef nigereoligosaccharides (NOS), a mixture of nigerose, nigerosyl glucose and nigerosyl maltose, was studied in vitro and in vivo in mice. Mitogen-induced proliferation of splenocytes from normal mice was augmented in a dose-dependent mallner by nigerose of NOS. NOS enhanced interleukin 12 aL-12) and interferon-7 (IFN-7) production by normal splenocytes in the presence of the potent IL-12 inducer, heat-killed Lac-tobaciUusplantaram L-137, in vitro. Consistent with the in vitro finding, L. plantarum L-137-induced IL-12 production and IL-2-induced IFN-y production were augmented in mice fed with a 14.6% NOS diet for 2 weeks compared with mice fed with a colltrol diet. Notably, mice fed with the NOS diet showed significantly longer suryival time than the control mice after the induction of an endogenous infectien by administering 5-fluorouracil in a Iethal dose. Taken together, these results suggest that NOS may exert immunopotentiating actiyity through the actiyation ef an IL-12-dependent T helper 1-like immune respense.

Enhancing immunity by dietary consumption of a probiotic lactic acid bacterium (Bifidobacterium lactis HN019): optimization and definition of cellular immune responses

“…Although these differences were signi®cant only for the NK cell data, the results do strongly suggest that the degree of immune enhancement imparted by B. lactis can be improved by delivering the probiotic in a suitable substrate. Whether these increased immune-enhancing effects re¯ect (indirectly) quantitative increases in number and gut colonizing ability of B. lactis is uncertain; aside from acting as a prebiotic, there is increasing evidence that oligosaccharides can interact directly with probiotic LAB and potentiate their immune-enhancing effects, both in vitro and in vivo (Murosaki et al, 1999). Oligosaccharides derived from milk are thought to have additional health bene®ts besides potentiating probiotic-mediated immune enhancement, including the prevention of pathogen colonization of the gut (Zopf & Roth, 1996;Roberfroid, 1998).…”

Pediococcus pentosaceusSn26 Inhibits IgE Production and the Occurrence of Ovalbumin-Induced Allergic Diarrhea in Mice

“…3,4) On the other hand, IFN-produced by Th1 cells suppresses IgE production by inhibiting the proliferation of Th2 cells. 5) IL-12, produced mainly by macrophages, stimulates differentiation from naïve T cells to Th1 cells. The balance of activation between Th1 and Th2 cells is considered critical in IgE production.…”

Preliminary Human Study for Possible Alteration of Serum Immunoglobulin E Production in Perennial Allergic Rhinitis with Fermented Milk Prepared with Lactobacillus gasseri TMC0356

“…Recently, several studies have suggested that lactic acid bacteria may act as prospective dietary immunoregulators. Some probiotic strains, such as L. casei Shirota and L. plantarum L-137, L. paracasei KW3110 appear to activate macrophages to secrete more pro-inflammatory cytokine IL-12 which can favor Th1 type immunity (6,18,24,33). Furthermore, these probiotic strains have been found to down-regulate antigen-induced serum IgE production in mice (18,21,25).…”