Immunophenotyping: Application to Safety Assessment

Wang, Xiaoting; Lebrec, Hervé

doi:10.1177/0192623317736742

Cited by 9 publications

(1 citation statement)

References 49 publications

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“…Cellular capture/phenotyping is a process for identifying and analyzing cell subpopulations by antigens or biomarkers present on cells, which can be used for cancer diagnostics by differentiating the cancer cells from normal cells, such as leukemia and lymphoma . Cellulose or surface‐modified cellulose have been widely adopted as immobilization substrates for biomolecules (proteins, antibodies, aptamers, etc.)…”

Section: Cell Capture/phenotyping On Paper Microfluidic Devicesmentioning

confidence: 99%

Paper Microfluidics for Cell Analysis

Yan

Miao

et al. 2018

Adv Healthcare Materials

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Paper microfluidics has attracted much attention since its first introduction around one decade ago due to the merits such as low cost, ease of fabrication and operation, portability, and facile integration with other devices. The dominant application for paper microfluidics still lies in point‐of‐care testing (POCT), which holds great promise to provide diagnostic tools to meet the ASSURED criteria. With micro/nanostructures inside, paper substrates provide a natural 3D scaffold to mimic native cellular microenvironments and create excellent biointerfaces for cell analysis applications, such as long‐term 3D cell culture, cell capture/phenotyping, and cell‐related biochemical analysis (small molecules, protein DNA, etc.). This review summarizes cell‐related applications based on various engineered paper microdevices and provides some perspectives for paper microfluidics‐based cell analysis.

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Section: Cell Capture/phenotyping On Paper Microfluidic Devicesmentioning

confidence: 99%

Paper Microfluidics for Cell Analysis

Yan

Miao

et al. 2018

Adv Healthcare Materials

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OMIP‐107: 8‐color whole blood immunophenotyping panel for the characterization and quantification of lymphocyte subsets and monocytes in swine

Arrigucci,

Patterson,

Dube

2024

Cytometry Pt A

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We developed this whole blood immunophenotyping panel with the aim to monitor and quantify major lymphocyte subsets (CD4+, CD8+, CD4+CD8+ αβ T cells, γδ‐T cells, B and NK cells) and monocytes in pigs. The panel involved the use of commercially available reagents, avoiding secondary antibody staining or in‐house antibody conjugations, with the aim to make the assay accessible and reproducible across laboratories. The assay is accurate, robust and represents a useful tool for immune monitoring of swine in the pharmacology and toxicology fields, or to monitor the immune status in response to vaccination and diseases.

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