Paper Microfluidics for Cell Analysis

Ma, Jun; Yan, Shiqiang; Miao, Chunyue; Li, Linmei; Shi, Weiwei; Liu, Xianming; Luo, Yong; Liu, Tingjiao; Lin, Baiquan; Wu, Wenming; Lu, Yao

doi:10.1002/adhm.201801084

Cited by 51 publications

(39 citation statements)

References 103 publications

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“…[ 241 ] In addition to the aforementioned materials, inexpensive microfluidic paper‐based platforms have been fabricated and tested for 3D cell culture. [ 242 ] For the foreseeable future, although PDMS will continue to provide an affordable rapid prototyping option for most researchers, the shift to new materials and techniques is primordial to create new approaches that meet all the necessary requirements for effective biomimetic microfluidic bioenvironmental platforms. Concerning the second main components of cell‐microfluidic platforms, a wide range of key functional components, including micromixers, microvalves, micropumps, sensing, control, and acting elements, can be used to create effective, complex, and powerful integrated microfluidic networks.…”

Section: Physically Active Bioreactors—main Typesmentioning

confidence: 99%

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Physically Active Bioreactors for Tissue Engineering Applications

et al. 2020

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Tissue engineering (TE) is a dynamic and growing scientific field that merges the knowledge of different areas such as biology, physics, medicine, and engineering. [1] The TE discipline was first coined at a National Science Foundation sponsored meeting in 1987, thus originating a field that employs life sciences and engineering with the purpose of developing biologic or synthetic supports to restore, keep, or enhance tissue functions or damaged organs. [2] The classical TE approach has been mainly focusing on associating cells with a supporting matrix, also called biomaterials or scaffolds, that essentially acts as a passive template for tissue formation in vitro by allowing cells to adhere, migrate, differentiate, and produce tissue. Usually, the cells are seeded onto the scaffolds and, occasionally, growth factors are also added. The combination of cells, growth factors, and scaffold is often referred as the TE triad. [3] Nevertheless, novel approaches in TE that include the application of a biophysical stimuli to the scaffolds through the use of a bioreactor are emerging. [4] Tissue engineering (TE) is a strongly expanding research area. TE approaches require biocompatible scaffolds, cells, and different applied stimuli, which altogether mimic the natural tissue microenvironment. Also, the extracellular matrix serves as a structural base for cells and as a source of growth factors and biophysical cues. The 3D characteristics of the microenvironment is one of the most recognized key factors for obtaining specific cell responses in vivo, being the physical cues increasingly investigated. Supporting those advances is the progress of smart and multifunctional materials design, whose properties improve the cell behavior control through the possibility of providing specific chemical and physical stimuli to the cellular environment. In this sense, a varying set of bioreactors that properly stimulate those materials and cells in vitro, creating an appropriate biomimetic microenvironment, is developed to obtain active bioreactors. This review provides a comprehensive overview on the important microenvironments of different cells and tissues, the smart materials type used for providing such microenvironments and the specific bioreactor technologies that allow subjecting the cells/ tissues to the required biomimetic biochemical and biophysical cues. Further, it is shown that microfluidic bioreactors represent a growing and interesting field that hold great promise for achieving suitable TE strategies.

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Section: Physically Active Bioreactors—main Typesmentioning

confidence: 99%

“…Thus, this section will focused on some microfluidic bioreactors that study the effect of dynamic stimuli as indicative examples of so many others applications that can be found in a number of excellent papers, already mentioned through this review. [ 221,233a,242,274 ]…”

Section: Physically Active Bioreactors—main Typesmentioning

confidence: 99%

Physically Active Bioreactors for Tissue Engineering Applications

et al. 2020

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“…Moreover, plastic substrates are widely used to map high resolution (<100 nm) conductive patterns for organic field effect transistors [51], and optoelectronic devices [52]. Despite the advantage of high resolution, photolithography did not gain popularity for resource-limited settings, because it is expensive, and requires large-footprint equipment, a special environment, and highly pure reagents for the procedure [53].…”

Section: Photolithographymentioning

confidence: 99%

Desktop Fabrication of Lab-On-Chip Devices on Flexible Substrates: A Brief Review

Qamar

Shamsi

2020

Micromachines

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Flexible microfluidic devices are currently in demand because they can be mass-produced in resource-limited settings using simple and inexpensive fabrication tools. Finding new ways to fabricate microfluidic platforms on flexible substrates has been a hot area. Integration of customized detection tools for different lab-on-chip applications has made this area challenging. Significant advancements have occurred in the area over the last decade; therefore, there is a need to review such interesting fabrication tools employed on flexible substrates, such as paper and plastics. In this short review, we review individual fabrication tools and their combinations that have been used to develop such platforms in the past five years. These tools are not only simple and low-cost but also require minimal skills for their operation. Moreover, key examples of plastic-based flexible substrates are also presented, because a diverse range of plastic materials have prevailed recently for a variety of lab-on-chip applications. This review should attract audience of various levels, i.e., from hobbyists to scientists, and from high school students to postdoctoral researchers, to produce their own flexible devices in their own settings.

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“…Prototypes are incompatible with industrial scale fabrication [1] nor easy to operate by untrained personnel and therefore, not accessible to resource-limited populations. Paper microfluidics [7][8][9] is the only major innovation in recent years to address the need for affordable and simple microfluidic devices to deliver POC health services in the developing world.…”

Section: Introductionmentioning

confidence: 99%

Label-free, High-throughput Assay of Human Dendritic Cells from Whole-blood Samples with Microfluidic Inertial Separation Suitable for Resource-limited Manufacturing

Yousuff¹,

Lim²,

Basha³

et al. 2020

Preprint

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Microfluidics technology has not impacted the delivery and accessibility of point of care health services like diagnosis of infectious disease diagnosis, monitoring health or delivering interventions. Most microfluidics prototypes from academic research are not easy to manufacture with industrial scale fabrication techniques and cannot be operated without complex manipulations of supporting equipment and additives such as labels or reagents. We propose a label- and reagent-free inertial spiral microfluidic device to separate red blood, white blood and dendritic cells from blood fluid for applications in health monitoring and immunotherapy. We demonstrate that using larger channel widths in the range of 200 to 600 µm allows separation of cells into multiple streams according to different size ranges and we utilize a novel technique to collect the closely separated focused cell streams without constricting the channel. When tested on actual human blood cells, 77% of dendritic cells were separated and 80% of cells remained viable after our assay. Our contribution is a method to adapt spiral inertial microfluidic designs to separate more than two cell types in the same device which is robust against clogging, simple to operate and suitable for fabrication and deployment in resource-limited populations.

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Paper Microfluidics for Cell Analysis

Cited by 51 publications

References 103 publications

Physically Active Bioreactors for Tissue Engineering Applications

Physically Active Bioreactors for Tissue Engineering Applications

Desktop Fabrication of Lab-On-Chip Devices on Flexible Substrates: A Brief Review

Label-free, High-throughput Assay of Human Dendritic Cells from Whole-blood Samples with Microfluidic Inertial Separation Suitable for Resource-limited Manufacturing

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