Immunophenotypic and Functional Characteristics of Haemopoietic Cells from Human Cord Blood

Abstract

Cord blood (CB) as a new source for bone marrow transplantation represents advantageous features concerning stem cell and leucocyte compartments and function. We attempted to get more information about the phenotypes and function of CB cells by investigating their cell surface markers and also the production of IL-2, IFN-gamma and IL-6 by mitogen and alloantigen stimulation. The CB cells were characterized by a low proportion of CD3+ T cells, CD4+ T subpopulation, activated T cells and CD3+CD16/CD56+ cytotoxic cells, suggesting reduced graft versus host potential. The significant increase of CD19/CD3 double positive cells and decrease of CD19/HLA-DR double positive mature B cells reflect that immature B cells exist in CB. In the functional studies, a 27- and 5-fold reduction was observed in the production of IFN-gamma by CB cells stimulated with PHA and allogeneic cells, respectively. The production of IL-2 in PHA-stimulated CB cells also showed a 50% determination. Decrease in the production of these cytokines by CB cells is supported by the decline of the proportion of CD3+ T cells. However, an increase was observed in the production of IL-6 by CB cells stimulated with allogeneic cells as compared with the controls. These results suggest a difference in the functional activity of the T helper cell subsets between the CB and peripheral blood and/or differences in the functional maturity of T helper cell subsets and B cells in these compartments.

Comparison of the lymphoid toxicities of mitobronitol and busulphan in mice: reduced B cell toxicity and improved thymic recovery as possible contributors to the reduced risk for complications following BMT with mitobronitol preconditioning

“…29 These data together give recipients, which indicated early recovery and supernormal percentage of IgM + B cells for the whole follow-up period. 13 support to the hypothesis that the reduction of post-BMT complications after DBM preconditioning could be due, in A sparing effect of DBM on certain specific immune responses was demonstrated earlier in murine studies, too. 15,20 Namely, addition to the relatively mild B cell toxicity, to a relative lack of long-term thymus toxicity.…”

“…13 In brief, the spleen was homogenized in Medium It is known that the rise of GVHD along with the various 199, and 5 × 10 5 cells in 50 l volume (in the wells of 96-bacterial and/or fungal infections are due to the myelowell U-bottom plates) were stained with 1:100 diluted rat ablation-induced immunosuppression that endures over a monoclonal anti-Thy-1.2 (30-H12) antibodies. Following long time after BMT.…”

Immunophenotypic and functional characterization of human umbilical cord blood mononuclear cells

“…[10][11][12][13][14][15][16][17][18][19] These data indicate that using only one-color CD34 analysis may not provide sufficient information, 12,15 but sensitive detection and enumeration of CD34 + cells is required. 12,13,15 The widespread use of CD34 expression as a measure of the transplant potential of hematopoietic tissue also means that it is important to establish any antibody-or tissue-related differences in levels of expression. 14 As a result of the different levels of detection by different MoAbs, the apparent number of CD34 + cells vary by approximately six-fold.…”

“…It has been demonstrated that committed hematopoietic progenitor cells are at least as numerous in cord blood as in normal BM. 13,15,16 Cord blood cells can be extensively expanded in suspension culture with different combinations of cytokines, 20 as well as in NOD/SCID mice 17 and other murine model in vivo. 21 Thus, immature hematopoietic stem cells and progenitor cells appear to be of good quality in cord blood.…”

Umbilical cord blood T lymphocytes are induced to apoptosis after being allo-primed in vitro

“…5 Much research has been conducted to clarify the characteristics of UCB lymphocytes. In summary, it has been reported that UCB has fewer CD3-positive T lymphocytes, [6][7][8][9][10] among which the majority express the naive, nonprimed CD45RA marker, and express fewer IL-2 receptor Correspondence: Dr M Hagihara, Department of Hematology and Rheumatology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan Received 29 April 1999; accepted 18 July 1999 molecules than adult PBL. 8 However, there is controversy about the immunoreactivity of UCB lymphocytes against allo-antigens.…”