Immunophenotypic and functional characterization of human umbilical cord blood mononuclear cells

Pálóczi, Katalin

doi:10.1038/sj.leu.2401318

Cited by 26 publications

(20 citation statements)

References 28 publications

(50 reference statements)

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“…Because of the limited cell numbers in UCB, microchimerism analysis in parallel with T-cell cloning could only be performed in 6 UCB samples (UCB 5,7,(9)(10)(11)20). In 3 of the 5 UCB samples with older brothers from which we were able to isolate HY-specific T cells in parallel, male cells were present in 1 or more cell subset(s).…”

Section: Male Microchimeric Cells Are Present In Different Cell Subsementioning

confidence: 99%

“…[8][9][10] It is generally believed that the differential clinical results of UCBT are because of the "naive and less Ag-experienced" status of UCB. 11 Yet, in vitro analyses of UCB samples demonstrate the presence of virus and minor histocompatibility (H) Ag-specific T cells, [12][13][14] and of various Th and cytotoxic T-cell responses in unmanipulated UCB. 15,16 Moreover, it has been shown that T cells expanded from UCB can be used for donor lymphocyte infusion as their in vitro Ag-specific responses are similar to adult peripheral blood.…”

Section: Introductionmentioning

confidence: 99%

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Transmaternal cell flow leads to antigen-experienced cord blood

Dierselhuis

Blokland

Pool

et al. 2012

Blood

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Section: Male Microchimeric Cells Are Present In Different Cell Subsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transmaternal cell flow leads to antigen-experienced cord blood

Dierselhuis

Blokland

Pool

et al. 2012

Blood

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“…Recently, it was shown that UCB contains various types of stem cells such as mesenchymal stem cells (MSCs) (Erices et al 2000), multipotent adult progenitor cells (Kogler et al 2004;Lee et al 2004), unrestricted somatic stem cells (Kogler et al 2004), and endothelial progenitor cells (Ingram et al 2004). The lymphocytes in UCB are functionally immature (Dimitriou et al 1998;Paloczi 1999) and UCB-derived progenitor cells can be maintained in long-term culture for more than 16 wk, suggesting the presence of very primitive hematopoietic cells (Dimitriou et al 1998). In addition, use of human UCB as a stem cell source has several advantages over other cell sources, for example, human UCB is abundant and can be obtained with non-invasive methods that raise none of the ethical issues related to other human adult stem cell sources (Gluckman 2009).…”

Section: Introductionmentioning

confidence: 99%

The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells

Lee

Nam

Park

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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Platelet-rich plasma (PRP) is an emerging therapeutic application because PRP contains various growth factors that have beneficial effects on tissue regeneration and engineering. Mesenchymal stem cells and PRP derived from peripheral blood have been well studied. In this study, we investigated the effects of PRP derived from human umbilical cord blood (UCB-PRP) on proliferation, alkaline phosphatase (ALP) activity, and osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and periodontal ligament stem cells (PDLSCs). Three types of dental stem cells were primarily isolated and characterized by flow cytometric analysis. Dental stem cells were exposed to various concentrations of UCB-PRP, which resulted in the proliferation of dental stem cells. Treatment with 2% UCB-PRP resulted in the highest level of proliferation. The ALP activity of DPSCs and PDLSCs increased following treatment with UCB-PRP in a dose-dependent manner up to a concentration of 2%. ALP activity decreased with higher concentration of UCB-PRP. The effects of UCB-PRP on calcium deposition were similar to those on proliferation and ALP activity. Treatment with 2% UCB-PRP resulted in the highest calcium depositions in DPSCs and PDLSCs; however, treatment with 1% UCB-PRP resulted in the highest calcium deposition in SHEDs. The concentrations of platelet-derived growth factor-AB and transforming growth factor-β1 in UCB-PRP were investigated and found to be comparable to the amounts in peripheral blood. Overall, UCB-PRP had beneficial effects on the proliferation and osteogenic differentiation of dental stem cells. Determination of the optimal concentration of UCB-PRP requires further investigation for clinical applications.

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“…Furthermore, our data indicate that the CD154 antigen expressed in newborn lymphocytes in response to cAMP‐elevating agents is functional and able to induce immunoglobulin class‐switching towards IgE production in the presence of IL‐4. It has been reported that neonatal B lymphocytes are deficient in mounting antibody responses which are attributed in part to a diminished capacity of neonatal T cells to provide cognate help to B cells because of an insufficient CD154 expression 1,2 , 20 . According to the present data, however, CD154 may be abundantly expressed on neonatal T cells under certain stimulation conditions.…”

Section: Discussionmentioning

confidence: 40%

Induction of functional CD154 (CD40 ligand) in neonatal T cells by cAMP‐elevating agents

et al. 2000

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SUMMARYA de®ciency of neonatal T lymphocytes to express CD154 antigen in response to ionomycin and phorbol 12-myrsistate 13-acetate (PMA) stimulation or after CD3 cross-linking has been described. In the present report we describe that CD45RA + newborn cells are able to synthesize and express CD154 at similar or even higher levels than adult cells in response to ionomycin and cAMPelevating agents which trigger the protein kinase A (PKA) -mediated metabolic pathway. Peak CD154 protein concentrations in newborn cells were found between 4 and 8 hr after stimulation with ionomycin and dibutyryl cAMP. These agents, however, did not induce expression of the early activation antigen CD69. Surface levels of CD154 did not correlate with speci®c mRNA concentration, indicating that dibutyryl cAMP up-regulates CD154 by acting at a posttranscriptional stage. The CD154 antigen induced by PKA activation of newborn cells was functional, since upon binding to CD40 on B lymphocytes in the presence of interleukin-4 (IL-4), it promoted immunoglobulin heavy-class switching to IgE. We also found a different pattern of cytokine production between neonatal and adult CD4 + T cells. In response to ionomycin and dibutyryl cAMP, cord blood cells were more prone than adult lymphocytes to secrete the T helper type 2-derived immunosuppressive cytokines IL-4 and IL-10. Taking into account that the feto± maternal environment is rich in cAMP-elevating agents, the reduced risk of graft versus host disease associated with cord blood trasplantation, as compared with the risk with adult bone marrow cell transplants, may be due to the bias of neonatal cells to differentiate towards the T helper type 2 functional cell subset.

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Immunophenotypic and functional characterization of human umbilical cord blood mononuclear cells

Cited by 26 publications

References 28 publications

Transmaternal cell flow leads to antigen-experienced cord blood

Transmaternal cell flow leads to antigen-experienced cord blood

The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells

Induction of functional CD154 (CD40 ligand) in neonatal T cells by cAMP‐elevating agents

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