Immunophafmacological Profile of Tei-3096: A New Immunomodulator

Abstract

TEI-3096 [6-p-chlorobenzyl-5H-2,3,6,7-terahydro-5,7-dioxothiazolo (3,2-a) pyrimidine], a novel thiazolopyrimidine compound has been shown to suppress adjuvant arthritis in rats without any effect on conventional inflammation. We examined the immuno-pharmacological profile of TEI-3096 in murine lymphocytes. The blastformation induced by Con A or LPS was inhibited by addition of 50-500 microM concentrations of TEI-3096. This compound suppressed elevated plaque-forming cell (PFC) response against T cell-dependent antigen. However, TEI-3096 had no effect either on normal PFC response or on antibody formation against T cell independent antigen. Although orally administered TEI-3096 had no obvious effect on anti-SRBC PFC response in normal mice, it normalized the colchicine-induced enhancement of antibody formation against TNP-HGG. TEI-3096 also enhanced the delayed type hypersensitivity in mice and rats. These results suggests that TEI-3096 restores the abnormal immune response. Therefore, it may be useful for the treatment of auto-immune disease such as rheumatoid arthritis.

Anti-arthritic and immunoregulatory effects of TI-31 on collagen-induced arthritis.

“…A total volume of 1.5 ml of the emulsion was injected intradermally at several sites on the back of rat on day 0. The volume of the left hind paw was measured by a plethysmometer (KN-357, Natsume Seisakusho Co., Ltd., Tokyo, Japan) (1). Results represent percent swelling of foot volume calculated from this formula:…”

“…TI-31 has immunomodulating properties. It inhibits the elevated antibody response seen in colchicine-treated mice without having any effect on antibody response in untreated mice (1). Furthermore, TI-31 potentiates the induction of suppressor T cells as well as inhibits the induction of helper T cells (4).…”