Immunoperoxidase Procedures for Rapid Detection of Bovine Viral Diarrhea-Mucosal Disease Virus Antigen.

Saino, Hitoshi; Watanabe, Hitoshi; Ikehata, Tsutomu

doi:10.1292/jvms.56.805

Cited by 6 publications

(1 citation statement)

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“…Virus isolation from sera was performed according to the immunoperoxidase monolayer assay as previously reported [28]. Bovine fetal muscular (BFM: originally produced in our laboratory) cells were maintained under the following conditions: EMEM supplemented with 3 mg/mL tryptose phosphate broth (Becton Dickinson, San Jose, CA, USA), 0.292 mg/mL L-glutamine (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 1.125 mg/mL sodium hydrogen carbonate (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), and 5% BVDV antigen-and antibody-free bovine serum (Japan Bio Serum, Hiroshima, Japan).…”

Section: Virus Isolation and The Immunoperoxidase Monolayer Assaymentioning

confidence: 99%

An Importance of Long-Term Clinical Analysis to Accurately Diagnose Calves Persistently and Acutely Infected by Bovine Viral Diarrhea Virus 2

Goto

Yaegashi

Fukunari

et al. 2021

Viruses

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Bovine viral diarrhea virus (BVDV) infection results in a wide variety of clinical manifestations and is a pathogen that is able to cause huge economic losses in the cattle industry worldwide. It is important to identify cattle that are persistently infected (PI) by BVDV within the herd as early as possible because PI animals are the main reservoir of the virus. In contrast, cattle who are acutely infected (AI) with BVDV show various clinical signs, but most cattle show either mild symptoms or are asymptomatic. In general, AI and PI animals can be distinguished by repeat testing within an interval of at least 21 days. However, we found a rare case of a BVDV2-infected AI animal with long-term viral presence, making it indistinguishable from PI through two tests within an interval of 21 days. As a result, we diagnosed one infected animal as AI after 35 days from the initial sample collection via multiple analyses. Our findings recommend performing an additional test using samples that have been collected after 14–21 days from the second sample collection in cases where it is difficult to accurately differentiate an AI diagnosis from a PI diagnosis after only two tests. Additionally, our analysis exhibits that monitoring the number of copies of viruses with similar genomes in the sera by means of quantitative real-time RT-PCR through several sample collections periods might be useful to distinguish AI from PI. Furthermore, our data suggest that the AI animals with a long-term viral presence who show test results similar to those of PI animals might be the result of a coincidental combination of various factors that are present in cattle fields. These findings provide useful information that can be used to improve the diagnosis of BVDV in the field.

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Section: Virus Isolation and The Immunoperoxidase Monolayer Assaymentioning

confidence: 99%