Immunopathology of Chronic Progressive Hepatitis in Nude Mice Infected with Low‐Virulent Mouse Hepatitis Virus

Midoro, Katsuo; Nakanaga, Kazue; Kyuwa, Shigeru; Fujiwara, Kôsaku

doi:10.1111/j.1348-0421.1989.tb02017.x

Cited by 3 publications

(1 citation statement)

References 29 publications

(48 reference statements)

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“…This results in incomplete deletion of T cells bearing T-cell receptor reactive to selfantigen (9, 13) and the induction of T-cell-dependent, organspecific autoimmune diseases (10,11,47). Finally, it has been reported that MHV infection induces T-cell growth and/or differentiation in athymic nude mice (33,45,50,51). Although the mechanism of this phenomenon is unknown, such a population of T cells might contain self-reactive T cells because the T cells have not been "educated" by the thymus.…”

Section: Discussionmentioning

confidence: 99%

Induction of self-reactive T cells after murine coronavirus infection

Kyuwa

Yamaguchi

Toyoda

et al. 1991

J Virol

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We studied the mechanism of in vitro spontaneous lymphokine production by spleen cells from mice injected intraperitoneally with murine coronavirus strain JHM 1 month after infection, when infectious virus had already been cleared from the spleens. Removal of either CD4+ T cells or Ia+ antigen-presenting cells (APC) from the spleen cells abrogated interleukin-2 (IL-2) production. Addition of anti-CD4 or anti-Iad monoclonal antibodies to the culture suppressed IL-2 production. These results suggest that the response involved typical receptor-mediated activation of T cells. Surprisingly, reciprocal mixing experiments with a coculture of T cells from infected mice and APC from either infected or naive mice resulted in the production of IL-2. The absence of viral antigens in spleen cells 1 month after infection, as indicated by their inability to induce the proliferation of T-cell clones specific for the viral antigens, suggest that the T cells from mice 1 month after infection were not responding to the viral antigens. The inoculum components other than the virus did not induce this immune response. We also found that the frequency of self-reactive but not alloreactive IL-2-producing T cells in the spleens of infected mice was 3to 10-fold higher than that in naive mice. These findings suggest that an increased frequency of self-reactive T cells which secrete IL-2 occurs following murine coronavirus infection. This may have important implications in the development of autoimmunelike phenomena following murine coronavirus infection.

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Section: Discussionmentioning

confidence: 99%

Induction of self-reactive T cells after murine coronavirus infection

Kyuwa

Yamaguchi

Toyoda

et al. 1991

J Virol

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Age-Related Changes in Susceptibility of Mice to Low-Virulent Mouse Hepatitis Virus(MHV-2-CC) Infection.

et al. 1997

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This study was performed to examine mouse age-dependent changes in susceptibility to MHV-2-CC-infection and participation of macrophages in such changes in BALB/c mice. One-week-old mice were fully susceptible (mortality, 100%), 2-week-old semi-susceptible (36%), and 3- and 4-week-old fully resistant (0%) to MHV-2-CC, respectively. Such age-dependent differences corresponded well with the differences in the virus titers in the liver, spleen and blood and in the severity of liver lesions. In 1-week-old mice with peritoneal exudate cells (PEC) transferred from 4-week-old mice and infected with MHV-2-CC, a slight prolongation of survival time was recorded, although there was no difference in mortality. In 3-week-old mice infected with MHV-2-CC after silica-treatment to suppress macrophages, there was no significant change in susceptibility. In macrophages infected with MHV-2-CC in vitro, the virus replicated better in macrophages obtained from younger mice. These results suggest that macrophages may play a small role in the age-related development of resistance to MHV-2-CC infection in BALB/c mice.

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Application of Nested Polymerase Chain Reaction to Detection of Mouse Hepatitis Virus in Fecal. Specimens during a Natural Outbreak in an Immunodeficient Mouse Colony.

et al. 1998

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The usefulness of RT-PCR for the detection of MHV in tissues and feces of experimentally infected animals has been reported, but it was unclear whether the method was also applicable for the detection of MHV during a natural outbreak. Enterotropic infection is considered to be the most common form of natural infection among various forms of MHV infection. In this paper, RT-nested PCR was performed to detect MHV excreted in the feces during an outbreak in an immunocompromised A/WySnJ mouse colony. The expected bands were amplified after nested PCR from 20 fecal samples out of 37. These results showed that RT-nested PCR could be applicable for the diagnosis for MHV natural infection.

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Immunopathology of Chronic Progressive Hepatitis in Nude Mice Infected with Low‐Virulent Mouse Hepatitis Virus

Cited by 3 publications

References 29 publications

Induction of self-reactive T cells after murine coronavirus infection

Induction of self-reactive T cells after murine coronavirus infection

Age-Related Changes in Susceptibility of Mice to Low-Virulent Mouse Hepatitis Virus(MHV-2-CC) Infection.

Application of Nested Polymerase Chain Reaction to Detection of Mouse Hepatitis Virus in Fecal. Specimens during a Natural Outbreak in an Immunodeficient Mouse Colony.

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