Immunomodulatory effects of Pediococcus acidilactici on BALB/C mice Immunosuppressed by Cyclophosphamide

Abstract

Background: The live microorganisms that present in food in addition dietary supplements called Probiotics they have beneficial effect in the human intestine. In the last years, Probiotics has become within treatment options for several disease included immune system. The advent and use of probiotics seem to be increasing day by day all over the world. Objective: The current study included detection of efficacy Pediococcus acidilactici as immunomodulatory in mice that treated with cyclophosphamide. Design: To detect efficacy of Pediococcus acidilactici as immunosuppressive model was used. This model was completed by taking 20 male BALB/C mice with six-weeks old to divide into (5) groups :(G 1) group is the normal control. (G2) group: the group that injected with cyclophosphamide. (G3) group: the immunosuppression plus Pediococcus acidilactici (6×108 CFU/ml). (G4) group: the immunosuppression plus Pediococcus acidilactici (6×104 CFU/ml). last (G5) group : is the group that treated with probiotics. Results: In the current study, thymus and spleen indicators were significantly higher in treated groups than those of (G1) group (NC) with (0.323±0.34) (P<0.05), also Macrophages phagocytosis showed a clear increase in the three treated groups (76.625±108, 72.125±1.65, 87.750±1.32) respectively with significantly (P<0.05), as a compared with (G1) group (NC). Conclusions: The current study reveals the ability of Pediococcus acidilactici (6×109) CFU/ml to accelerate the healing of cyclophosphamide immunosuppressive mice. So, the activity role of probiotic strain as immunomodulator in this study leads us to urge the use it as an alternative treatment for chemical drugs that used against immunosuppression.

The combined effect of Weisella cibaria and Fusarium oxysporum nanoparticles on cervical cancer cells

“…18 and Mohsin et al 19 showed that Weisella always appears in culture as white colonies, small, smooth and shiny. The presence of several fractions (contained proteins with a molecular mass less than 500 Da) may be the cause of T1 inhibition of studied bacteria; in vitro findings related to the peptidic nature of the LAB species linked to bacteria inhibition 20,21,22 , or may be occurrence the inhibition due to that some compounds work on the cell membrane and depolarize it, So, the cell wall synthesis inhibition, these compounds known as Diacetyl organic acids (lactic , formic, acetic and propionic) the pH of the medium was reduced as a result of this, So can compete for nutrients 20,23,24 .…”

“…Previous studies pointed out the antibacterial activity of LAB sp. because of hydroxyl fatty acids and phenolic compounds , these antimicrobial agents were conducted to act as decrease the electrochemical proton gradient, and H2O2 by peroxidation of lipids membrane ,so, changing the permeability of the cell membrane, resulting in disruption of the substrate transport mechanism [21][22][23] .…”