Immunomodulatory Effects ofLactobacillus plantarumLp62 on Intestinal Epithelial and Mononuclear Cells

Abstract

Probiotic lactic acid bacteria are known for their ability to modulate the immune system. They have been shown to inhibit inflammation in experiments with animal models, cell culture, and clinical trials. The objective of this study was to elucidate the anti-inflammatory potential of Lactobacillus plantarum Lp62, isolated from cocoa fermentation, in a cell culture model. Lp62 inhibited IL-8 production by Salmonella Typhi-stimulated HT-29 cells and prevented the adhesion of pathogens to these epithelial cells. The probiotic strain was able to modulate TNF-α, IL1-β, and IL-17 secretion by J774 macrophages. J774 activation was reduced by coincubation with Lp62. PBMC culture showed significantly higher levels of CD4+CD25+ T lymphocytes following treatment with Lp62. Probiotics also induced increased IL-10 secretion by mononuclear cells. L. plantarum Lp62 was able to inhibit inflammatory stimulation in epithelial cells and macrophages and activated a tolerogenic profile in mononuclear cells of healthy donors. These results indicate this strain for a possible application in the treatment or prevention of inflammatory diseases.

Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro

“…However, unresolved questions concern the scopes of local and systemic effects as well as the molecular pathways responsible for interactions with host cells. The local immunomodulatory contributions of LAB have been demonstrated in several intestinal mucosal epithelial cell lines and mucosal immune cells (e.g., T lymphocytes, dendritic cells, and monocytes-macrophages) [33,34].…”

A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics

“…Immunomodulatory capacity is considered as an additional factor for the selection of probiotics. A number of in vitro probiotic studies have investigated the anti-inflammatory properties of probiotics to modulate the release of pro- and anti-inflammatory cytokines including IL-6, IL-8, TNF-α and IL-10 15 , 17 , 43 , 44 . In our study, most of the tested strains downregulated the pro-inflammatory IL-8, and TNF-α and upregulated IL-10 levels when compared to controls, but the rate and extent of immunomodulatory effect varied between the strains and under different conditions.…”

Effect of riboflavin-producing bacteria against chemically induced colitis in mice

“…) and the improvement of the host's immune response (Ferreira dos Santos et al . ). In addition, some LABs have also been reported to have the capacity to produce antioxidant vitamins such as riboflavin (vitamin B2) (Burgess et al .…”

“…Probiotics are defined as 'live micro-organisms, which when administered in adequate amounts confer a beneficial health effect on the host' (FAO/WHO, 2001). Among probiotics, certain strains of lactic acid bacteria (LAB) have been shown to exert beneficial effects against IBD via different mechanisms, including the modulation of the intestinal microbiota and the alteration of their metabolic properties (de Moreno de LeBlanc and LeBlanc 2014), the reduction of oxidative stress (del Carmen et al 2014) and the improvement of the host's immune response (Ferreira dos Santos et al 2016). In addition, some LABs have also been reported to have the capacity to produce antioxidant vitamins such as riboflavin (vitamin B2) (Burgess et al 2006;LeBlanc et al 2011b;Capozzi et al 2012).…”