A human origin strain <i>Lactobacillus acidophilus</i> DDS-1 exhibits superior <i>in vitro</i> probiotic efficacy in comparison to plant or dairy origin probiotics

Vemuri, Ravichandra; Shinde, Tanvi; Shastri, Madhur D.; Perera, Agampodi Promoda; Tristram, Stephen; Martoni, Christopher; Gundamaraju, Rohit; Ahuja, Kiran; Ball, MJ

doi:10.7150/ijms.25004

Cited by 38 publications

(38 citation statements)

References 46 publications

(91 reference statements)

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“…M. smithii produces methane from the byproducts of bacterial fermentation and is present in up to 95% of fecal samples from human subjects [113]. Interestingly, M. smithii's abundance was found to be stable over time, even after major changes in diet [14,114]. In the human gut, methanogenic archaea may impact host metabolism, especially energy homeostasis and may contribute to obesity [79], by syntrophic interactions with gut bacteria, which increase short-chain fatty acid production and contribute to an excess of calories available to the host [115].…”

Section: Gut Archaeamentioning

confidence: 99%

“…For example, probiotics (single or multi-strained) are considered natural, safe, and beneficial modulators of the gut microbial composition, but their efficacy is strain-and dose-dependent and not all probiotics function against all disorders and diseases [154]. Fundamentally, to confer health benefits a probiotic needs to survive the acidic gastric digestion, other digestive enzymes, reach the intestine and colonize on the intestinal epithelium in good numbers [114]. In most of the probiotic trials, the vehicles for probiotic intake used are yoghurt, biscuits, bars, and capsules, per oral, which is not consistent across the studies and can potentially confound the outcomes [155].…”

Section: Modulation Of the Microbiome And Related Concernsmentioning

confidence: 99%

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Beyond Just Bacteria: Functional Biomes in the Gut Ecosystem Including Virome, Mycobiome, Archaeome and Helminths

et al. 2020

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Gut microbiota refers to a complex network of microbes, which exerts a marked influence on the host’s health. It is composed of bacteria, fungi, viruses, and helminths. Bacteria, or collectively, the bacteriome, comprises a significant proportion of the well-characterized microbiome. However, the other communities referred to as ‘dark matter’ of microbiomes such as viruses (virome), fungi (mycobiome), archaea (archaeome), and helminths have not been completely elucidated. Development of new and improved metagenomics methods has allowed the identification of complete genomes from the genetic material in the human gut, opening new perspectives on the understanding of the gut microbiome composition, their importance, and potential clinical applications. Here, we review the recent evidence on the viruses, fungi, archaea, and helminths found in the mammalian gut, detailing their interactions with the resident bacterial microbiota and the host, to explore the potential impact of the microbiome on host’s health. The role of fecal virome transplantations, pre-, pro-, and syn-biotic interventions in modulating the microbiome and their related concerns are also discussed.

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Section: Gut Archaeamentioning

confidence: 99%

Section: Modulation Of the Microbiome And Related Concernsmentioning

confidence: 99%

Beyond Just Bacteria: Functional Biomes in the Gut Ecosystem Including Virome, Mycobiome, Archaeome and Helminths

et al. 2020

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“…Probiyotik suşların her şeyden önce toksin üretmeyen ve vücuda alındıklarında sağlığa zarar vermeyen, yani GRAS (Generally Regarded As Safe) statüsündeki mikroorganizmalardan seçilmiş olması gerekmektedir. Ayrıca, bir mikroorganizmanın probiyotik olarak gıdada kullanılabilmesi için; tercihen insan orijinli olması, patojen olmaması, mide asidine ve safra tuzuna dayanıklı olması, bağırsak yüzeyine tutunabilmesi, gastrointestinal sistemde kısa da olsa yaşamını devam ettirebilmesi, antimikrobiyal karakterde bileşikler üretebilmesi, antibiyotik direncinin olmaması, bağışıklık sistemini uyarması, metabolik etkiler gösterebilmesi, (kolesterol asimilasyonu, laktaz aktivitesi, vitamin üretimi) ve teknolojik süreçlere dayanıklı olması beklenmektedir (Collins et al, 1998;Dunne et al, 2001;Önal-Darılmaz, 2010;Uymaz, 2010;Erem vd., 2013;Kechagia et al, 2013;Çomak-Göçer vd., 2016 (Vemuri et al, 2018).…”

Section: Sonunclassified

“…Bağırsak yüzeyine tutunmaya yönelik araştırmalarda, normal insan ince bağırsak villus hücrelerinin özelliklerine sahip olan Caco-2, HT-29, LS174T ve fetal I-407 hücre hatları, hücre kültürü çalışmalarında yaygın olarak kullanılmaktadır. Bununla birlikte adhezyonun incelenmesi dışında hücrelerin probiyotiklere gösterdiği reaksiyonu incelemek için de hücre kültürü kullanılmaktadır (Blum et al, 1999;Dunne et al, 2001;Brito et al, 2012;Argyri et al, 2013;Anonymous, 2018b;De Melo Pereira et al, 2018;Vemuri et al, 2018).…”

Section: Bağırsak Yüzeyine Tutunmaunclassified

Probiyotikler ve Belirlenmelerinde Kullanılan in vitro Testler

Bilginer¹,

Çeti̇n²

2019

Atatürk Üniversitesi Ziraat Fakültesi Dergisi

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ÖZ: Fonksiyonel gıdalara ve sağlıklı beslenmeye olan ilginin her geçen gün artması ile birlikte probiyotikler, bilim dünyasının önemli çalışma alanlarından biri haline gelmiştir. İnsan ve probiyotikler arasındaki mutual ilişkinin zamanla yeni boyutlar kazanması, konu üzerindeki çalışma sayısını ve probiyotik ürünlere olan ilgiyi daha da artırmaktadır. Probiyotik bir ürün üretmeden önce, o üründe kullanılacak mikroorganizmaların probiyotik olup olmadığının tespitinde ve güvenlik değerlendirmelerinde, in vitro ve in vivo testlerden faydalanılmaktadır. Bu derlemede, muhtemel probiyotik suşun insan gastrointestinal sistemiyle uyumuna yönelik olarak kullanılan; mide ortamına direnç, safra tuzuna tolerans, antibiyotik direnci, antagonistik etki, kolesterol asimilasyonu, bağırsak yüzeyine yapışma ve model gastrointestinal sistemler hakkında özet bilgi verilmeye çalışılmıştır.

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“…However, the relationship between probiotics and gut microbiota in aging is unclear. Recently, we reported that the human origin strain Lactobacillus acidophilus DDS‐1 demonstrated superior probiotic traits in an in vitro setting . Taking this further, we evaluated the changes in microbial ecology, and immune and metabolite status in young and aging mice using DDS‐1.…”

Section: Modulation Of Gut Microbiota and Metabolic Profile After Supmentioning

confidence: 99%

Luminal basic science

2018

J of Gastro and Hepatol

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Aim: The gut microbiota is a contributing factor in overall health throughout the human lifecycle. Probiotics are natural, safe, and beneficial modulators of gut microbiota. However, the relationship between probiotics and gut microbiota in aging is unclear. Recently, we reported that the human origin strain Lactobacillus acidophilus DDS-1 demonstrated superior probiotic traits in an in vitro setting. 1 Taking this further, we evaluated the changes in microbial ecology, and immune and metabolite status in young and aging mice using DDS-1. Methods: A total of 32 C57BL/6 J mice were used in this study, which were divided into four groups based on age and treatments: young control and treatment, and aging control and treatment groups. Each group had eight mice. Each mouse was caged individually and supplemented with probiotic DDS-1 in the normal chow for 4 weeks. We sequenced bacterial 16S rRNA genes V1-V3 region of fecal, cecal, and mucosal samples. Cytokine analysis was performed on colonic and blood samples. All clinical parameters, such as body weights, colon length, and colon and spleen weights, were recorded. Colonic sections were routinely stained with hematoxylin and eosin. Metabolic and short-chain fatty acid profiling was performed to understand the metabolic status of the mice. Results: Supplementation with DDS-1 modulated the microbiota community and promoted the abundance of beneficial bacteria, such as Akkermansia and S-24-7. At phylum level, Bacteroidetes levels were increased from 63 ± 0.30 to 69 ± 0.75, and for Firmicutes from 28 ± 0.44 to 30 ± 0.28 after DDS-1 treatment. Interestingly, Verrucomicrobia levels were increased from 3 ± 0.48% to 4.0 ± 26% at phylum level, and the only identifiable species under Verrucomicrobia was Akkermansia municiphilia. There were no significant differences in clinical parameters. Although there was no change in overall colonic anatomy, the goblet cells were more in number in probiotic-treated groups. DDS-1 was able to reduce proinflammatory cytokines IL-1, 2, 5, IL-1β, and MIP 1α and increase anti-inflammatory IL-10 levels in the serum samples. At the tissue level, IL-1, IL-1β, and IL-12 levels were downregulated in treated groups. Metabolomics analysis correlated well with the microbiota findings. Conclusion: DDS-1 supplementation promoted the growth of beneficial composition of gut microbiota. These results show the potential of probiotics for gut health in the elderly human population. Reference 1 Vemuri R, Shinde T, Shastri MD et al. A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics. Int. J. Med. Sci. 2018; 15(9): 840-8.

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A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics

Cited by 38 publications

References 46 publications

Beyond Just Bacteria: Functional Biomes in the Gut Ecosystem Including Virome, Mycobiome, Archaeome and Helminths

Beyond Just Bacteria: Functional Biomes in the Gut Ecosystem Including Virome, Mycobiome, Archaeome and Helminths

Probiyotikler ve Belirlenmelerinde Kullanılan in vitro Testler

Luminal basic science

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