Immunomodulatory Effects of Eckol, a Pure Compound of Ecklonia Cava, on Dendritic Cells

Kim, Mi Hyoung; Joo, Hong‐Gu

doi:10.4110/in.2006.6.4.199

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…Generally, dendritic cells localized in healthy livers display a predominant tolerogenic phenotype, which is characterized by a low ability to endocytose antigens and to stimulate T lymphocytes, together with the higher production of immune- inhibitory mediators such as IL-10 [ 30 , 32 ]. Coincidentally, a previous study [ 33 ] had reported the in vitro immunomodulatory effects of eckol on the dendritic cells, one of which was that eckol could protect dendritic cells against apoptosis in a concentration dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

Liu

Zhang

et al. 2018

Marine Drugs

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Several in vitro studies have shown the potential hepatoprotective properties of eckol, a natural phlorotannin derived from the brown alga. However, the in vivo hepatoprotective potential of eckol has not been determined. In this study, we performed an in vivo study to investigate the protective effect of eckol and its possible mechanisms on the carbon tetrachloride (CCl4)-induced acute liver injury model in mice. Results revealed that eckol pre-treatment at the dose of 0.5 and 1.0 mg/kg/day for 7 days significantly suppressed the CCl4-induced increases of alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in serum and meliorated morphological liver injury. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) analysis showed that the number of positive apoptotic hepatocytes in the eckol-treated group was lower than that in the CCl4 model group. Western blotting analysis also demonstrated the enhanced expression of bcl-2 and suppressed expression of cleaved caspase-3 by eckol. The CCl4-induced oxidative stress in liver was significantly ameliorated by eckol, which was characterized by reduced malondialdehyde (MDA) formations, and enhanced superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and glutathione (GSH) content. Moreover, the CCl4-induced elevations of pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were markedly suppressed in the eckol-treated group. However, eckol enhanced the level of IL-10, a potent anti-inflammatory cytokine, and recruited CD11c+ dendritic cells into the liver tissues of CCl4-treated mice. These results indicated that eckol has the protective effect on CCl4-induced acute liver injury via multiple mechanisms including anti-apoptosis, anti-oxidation, anti-inflammation and immune regulation.

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Section: Resultsmentioning

confidence: 99%

Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

Liu

Zhang

et al. 2018

Marine Drugs

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“…As the most important professional antigen-presenting cells contributing to the regulation of inflammation and immunity, dendritic cells (DCs) play a crucial role in intestinal immune homeostasis, especially in inducing and maintaining immune tolerance to prevent overt inflammatory and immune responses to the gut microbiota [35,37]. Eckol has been found to protect in vitro DCs against apoptosis [38], and to recruit DCs into the liver tissues in carbon tetrachloride-treated mice [12], suggesting its potential immunomodulatory role associated with DCs.…”

Section: Eckol Modulated the Infiltration Of Immune Cells In Dss-chal...mentioning

confidence: 99%

Protective Effect and Mechanisms of Eckol on Chronic Ulcerative Colitis Induced by Dextran Sulfate Sodium in Mice

Liao,

Wei,

et al. 2023

Marine Drugs

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The use of functional foods and their bioactive components is receiving increasing attention as a complementary and alternative therapy for chronic ulcerative colitis (UC). This study explored the protective effect and mechanisms of Eckol, a seaweed-derived bioactive phlorotannin, on the dextran sodium sulfate (DSS)-induced chronic UC in mice. Eckol (0.5–1.0 mg/kg) reduced DSS-enhanced disease activity indexes, and alleviated the shortening of colon length and colonic tissue damage in chronic UC mice. The contents of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were significantly decreased, and the level of anti-inflammatory IL-10 was enhanced in the serum and colonic tissues collected from Eckol-treated mice compared with the DSS controls. Eckol administration significantly reduced the number of apoptotic cells and the expression of cleaved Caspase-3, and increased the B-cell lymphoma-2 (Bcl-2)/B-cell lymphoma-2- associated X (Bax) ratio in DSS-challenged colons. There were more cluster of differentiation (CD)11c+ dendritic cells and CD8+ T cells, and less CD4+ T cells infiltrated to inflamed colonic tissues in the Eckol-treated groups. Expression of colonic Toll-like receptor 4 (TLR4), nuclear factor kappa-B (NF-κB) p65, phosphorylated-signal transducer and activator of transcription (pSTAT)3 was significantly down-regulated by Eckol compared with the DSS-challenged group. In conclusion, our data suggest that Eckol appeared to be a potential functional food ingredient for protection against chronic UC. The anti-colitis mechanisms of Eckol might be attributed to the down-regulation of the TLR4/NF-κB/STAT3 pathway, inhibition of inflammation and apoptosis, as well as its immunoregulatory activity.

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Macroalgae: Marine players in vaccinology

Trujillo,

Monreal-Escalante,

Ramos-Vega

et al. 2024

Algal Research

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Immunomodulatory Effects of Eckol, a Pure Compound of Ecklonia Cava, on Dendritic Cells

Cited by 4 publications

References 9 publications

Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

Protective Effect and Mechanisms of Eckol on Chronic Ulcerative Colitis Induced by Dextran Sulfate Sodium in Mice

Macroalgae: Marine players in vaccinology

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