Immunomodulatory Effect of Tonsil-Derived Mesenchymal Stem Cells in a Mouse Model of Allergic Rhinitis

Samivel, Ramachandran; Kim, Eun Hee; Chung, Young-Jun; Mo, Ji‐Hun

doi:10.2500/ajra.2015.29.4216

Cited by 31 publications

(24 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, studies for T-MSCs described an immunomodulatory effect on degenerative or inflammatory diseases [17][18][19][20]. Our previous study also demonstrated an immunomodulatory effect of T-MSCs in a mouse model of allergic rhinitis [21]. However, conditioned media released by T-MSCs (T-MSCs-CM) are more feasible for preparation and application than T-MSCs themselves.…”

Section: Introductionmentioning

confidence: 89%

“…Therefore, in this study, we investigated the immunomodulatory effect of T-MSCs-CM on the allergic rhinitis mouse model and also evaluated the inhibitory mechanism of T cell activation by T-MSCs-CM. [21], we harvested tonsil tissue after tonsillectomy and it was cultured in stem cell-enrichment condition, which consisted of RPMI-1640 media (Gibco, Grand Island, NY) with 10% fetal bovine serum (Gibco) and 1% penicillin and streptomycin (Gibco) at a density of 2 × 10 4 cells/cm 2 . The following day, the cells were switched to serum-free RPMI-1640 media.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model

Park

Kim

Bae

et al. 2020

Mediators of Inflammation

Self Cite

View full text Add to dashboard Cite

Background and Purpose. Recently, tonsil-derived mesenchymal stem cells (T-MSCs) have attracted great attention in various medical fields due to easier harvest of T-MSCs and more immunomodulatory effects than adipose-derived MSCs. However, there was still little evidence of the effect of conditioned media from T-MSCs (T-MSCs-CM) on allergic rhinitis (AR). Therefore, we investigated the impact of T-MSCs-CM on an AR mouse model. Methods. We isolated T-MSCs from human palatine tonsil and evaluated the ingredients of T-MSCs-CM. The effect of T-MSCs-CM was evaluated in the AR mouse model that was randomly divided into five groups (negative control, positive control, and T-MSCs-CM treated (0.1 mg, 1 mg, and 10 mg)). To investigate the therapeutic effect, we analyzed rhinitis symptoms, serum immunoglobulin (Ig), inflammatory cells, and cytokine expression. We also assessed T cell receptor signal, including MAP kinase (ERK/JNK), p65, and NFAT1. Results. We identified the increment of TGF-β1, PGE2, and HGF in the T-MSCs-CM. In an animal study, the T-MSCs-CM-treated group showed significantly reduced allergic symptoms and infiltration of eosinophils and neutrophils in the nasal mucosa, whereas there was no significant difference in total IgE and the OVA-specific IgE level. Additionally, we found that the 10 mg T-MSCs-CM-treated group showed a significantly decreased IL-4 mRNA expression, compared to the (+) Con group. In the analysis of T cell receptor signal, the phosphorylation of MAP kinases, translocation of p65, and activation of NFAT1 were inhibited after T-MSCs-CM. Conclusions. Our findings suggest that T-MSCs-CM showed a partial immunomodulatory effect on the AR mouse model by the inhibition of T cell activation via MAP kinase, p65, and NFAT1.

show abstract

Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model

Park

Kim

Bae

et al. 2020

Mediators of Inflammation

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to those distinct characteristics, TMSC exhibit immune regulatory activity like other MSC. Previously, TMSC have been shown to have an immunomodulatory effect on allergic rhinitis in mice via both the reduction of Th2 cytokines and ovalbumin-specific immunoglobulin E secretion from B cells [ 26 ]. In this report, the authors suggested the inhibition of innate cytokines IL-25 and IL-33 as the underlying mechanism.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of tonsil-derived mesenchymal stem cells in dextran sulfate sodium-induced experimental murine colitis

Song

Lee

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

The therapeutic potential of tonsil-derived mesenchymal stem cells (TMSC) prepared from human tonsillar tissue has been studied in animal models for several diseases such as hepatic injury, hypoparathyroidism, diabetes and muscle dystrophy. In this study, we examined the therapeutic effects of TMSC in a dextran sulfate sodium (DSS)-induced colitis model. TMSC were injected in DSS-induced colitis mice via intraperitoneal injection twice (TMSC[x2]) or four times (TMSC[x4]). Control mice were injected with either phosphate-buffered saline or human embryonic kidney 293 cells. Body weight, stool condition and disease activity index (DAI) were examined daily. Colon length, histologic grading, and mRNA expression of pro-inflammatory cytokines, interleukin 1β (IL-1β), IL-6, IL-17 and tumor necrosis factor α, and anti-inflammatory cytokines, IL-10, IL-11 and IL-13, were also measured. Our results showed a significant improvement in survival rates and body weight gain in colitis mice injected with TMSC[x2] or TMSC[x4]. Injection with TMSC also significantly decreased DAI scores throughout the experimental period; at the end of experiment, almost complete reversal of DAI scores to normal was found in colitis mice treated with TMSC[x4]. Colon length was also significantly recovered in colitis mice treated with TMSC[x4]. However, histopathological alterations induced by DSS treatment were not apparently improved by injection with TMSC. Finally, treatment with TMSC[x4] significantly reversed the mRNA levels of IL-1β and IL-6, although expression of all pro-inflammatory cytokines tested was induced in colitis mice. Under our experimental conditions, however, no apparent alterations in the mRNA levels of all the anti-inflammatory cytokines tested were found. In conclusion, our findings demonstrate that multiple injections with TMSC produced a therapeutic effect in a mouse model of DSS-induced colitis.

show abstract

“…Eotaxin-2 expression was evident in all analysed subgroups with a significant upregulation in adenoids, suggesting an involvement of Th2- immune responses as eotaxin-2 which is known to play a crucial role in the pathogenesis of atopic diseases involving Th 2- cell activation 41 . Significant upregulation of eotaxin-2 has been observed in bronchial asthma 42 and allergic rhinitis 43 . However, the significance of eotaxin-2 expression in lymphatic tissue of the Waldeyer’s tonsillar ring still largely remains uncertain, like their role in the maturation of the adaptive immune system in the mucosa of the upper aerodigestive tract.…”

Section: Discussionmentioning

confidence: 99%

Differential chemokine expression patterns in tonsillar disease

Mandapathil

Beier

Graefe

et al. 2018

Acta Otorhinolaryngol Ital

View full text Add to dashboard Cite

Immunomodulatory Effect of Tonsil-Derived Mesenchymal Stem Cells in a Mouse Model of Allergic Rhinitis

Cited by 31 publications

References 25 publications

The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model

The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model

Therapeutic potential of tonsil-derived mesenchymal stem cells in dextran sulfate sodium-induced experimental murine colitis

Differential chemokine expression patterns in tonsillar disease

Contact Info

Product

Resources

About