The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model

Park, In-Su; Kim, Ji Hye; Bae, Jun-Sang; Kim, Dong Kyu; Mo, Ji‐Hun

doi:10.1155/2020/6982438

Cited by 10 publications

(17 citation statements)

References 35 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…De Corgnol et al used olfactory ensheathing cells, in rats with vagus nerve section, and reported the reinnervation in the vocal folds [177]. Transplantation of tonsil-derived stem cells in mice allergic rhinitis model showed significantly decreased allergic symptoms, a reduced infiltration of eosinophils and neutrophils in the nasal mucosa and a significantly declined IL-4 mRNA expression that can demonstrate the immunomodulatory effect of tonsil-derived stem cells via inhibition of T cell activation, mitogen-activated protein (MAP) kinase, p65, and NFAT1 [178]. The intravenous injection of tonsil-derived stem cells, in a model of allergic rhinitis in mice, induced by ovalbumin, showed a significantly reduced allergic symptoms, a decrease in eosinophil infiltration, a decline in total serum and immunoglobulin E (IgE), a reduction in the nasal and systemic Th2 cytokine profile, and finally, an inhibition in allergic inflammation [179].…”

Section: In Vivo Studies In Rhinologymentioning

confidence: 95%

Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology

Kaboodkhani

Mehrabani

Karimi‐Busheri

2021

JCM

View full text Add to dashboard Cite

Otorhinolaryngology enrolls head and neck surgery in various tissues such as ear, nose, and throat (ENT) that govern different activities such as hearing, breathing, smelling, production of vocal sounds, the balance, deglutition, facial animation, air filtration and humidification, and articulation during speech, while absence of these functions can lead to high morbidity and even mortality. Conventional therapies for head and neck damaged tissues include grafts, transplants, and artificial materials, but grafts have limited availability and cause morbidity in the donor site. To improve these limitations, regenerative medicine, as a novel and rapidly growing field, has opened a new therapeutic window in otorhinolaryngology by using cell transplantation to target the healing and replacement of injured tissues. There is a high risk of rejection and tumor formation for transplantation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs); mesenchymal stem cells (MSCs) lack these drawbacks. They have easy expansion and antiapoptotic properties with a wide range of healing and aesthetic functions that make them a novel candidate in otorhinolaryngology for craniofacial defects and diseases and hold immense promise for bone tissue healing; even the tissue sources and types of MSCs, the method of cell introduction and their preparation quality can influence the final outcome in the injured tissue. In this review, we demonstrated the anti-inflammatory and immunomodulatory properties of MSCs, from different sources, to be safely used for cell-based therapies in otorhinolaryngology, while their achievements and challenges have been described too.

show abstract

Section: In Vivo Studies In Rhinologymentioning

confidence: 95%

Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology

Kaboodkhani

Mehrabani

Karimi‐Busheri

2021

JCM

View full text Add to dashboard Cite

show abstract

“…adipose-derived MSCs [11][12][13][14]. Some studies showed that exogenously administered T-MSCs migrate to damaged tissue sites and participate in tissue repair by immune regulatory functions in a mouse model of liver injury [15,16].…”

Section: Characterization Of Tonsil Derived Mesenchymal Stem Cellmentioning

confidence: 99%

“…Tonsil-derived mesenchymal stem cells (T-MSCs) have recently been introduced and have been getting a great deal of attention in various medical fields due to them being easier to harvest than BM-MSCs. They also have more immunomodulatory effects than adipose-derived MSCs [11][12][13][14]. Some studies showed that exogenously administered T-MSCs migrate to damaged tissue sites and participate in tissue repair by immune regulatory functions in a mouse model of liver injury [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…Some studies showed that exogenously administered T-MSCs migrate to damaged tissue sites and participate in tissue repair by immune regulatory functions in a mouse model of liver injury [15,16]. Other studies have also demonstrated an immunomodulatory effect of T-MSCs in a mouse model of allergic rhinitis [14,17]. Moreover, several in vitro studies revealed that T-MSCs exert immunomodulatory effects on T lymphocyte proliferation [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

IDO and CD40 May Be Key Molecules for Immunomodulatory Capacity of the Primed Tonsil-Derived Mesenchymal Stem Cells

Lee

Jung

2021

IJMS

Self Cite

View full text Add to dashboard Cite

Background: Tonsil-derived mesenchymal stem cells (T-MSCs) were reported to have suppressive effect on T cells, yet much remains unknown about the underlying mechanisms supporting this effect. We investigated the underlying mechanism of the immunomodulatory effect of T-MSCs on immune cell proliferation and cytokine production. Methods: We isolated T-MSCs from human palatine tonsil and evaluated the immunomodulatory capacity using RT-PCR, ELISA, and flow cytometry. Additionally, we assessed the expression of various soluble factors and several costimulatory molecules to detect the priming effect on T-MSCs. Results: T-MSCs significantly inhibited the immune cell proliferation and cytokine expression (TNF-α and IFN-γ) in the direct co-culture, but there was no suppressive effect in indirect co-culture. Additionally, we detected a remarkably higher expression of indoleamine 2,3-dioxygenase (IDO) in the primed T-MSCs having co-expression CD40. Moreover, immune cells or CD4+ T cells showed lower TNF-α, IFN-γ, and IL-4 expression when the primed T-MSC were added; whereas those findings were reversed when the inhibitor for IDO (not IL-4) or CD40 were added. Furthermore, T-bet and GATA3 levels were significantly decreased in the co-cultures of the primed T-MSCs and CD4+ T cells; whereas those findings were reversed when we added the neutralizing anti-CD40 antibody. Conclusions: Primed T-MSCs expressing IDO and CD40 may have immunomodulatory capacity via Th1-mediated and Th2-mediated immune response.

show abstract

“…4–7 Several previous studies have also shown that T-MSCs and conditioned media released by T-MSCs (T-MSCs-CM) have an anti-allergic effect. 8,9…”

Section: Introductionmentioning

confidence: 99%

Increased Anti-Allergic Effects of Secretome of Low-Level Light Treated Tonsil-Derived Mesenchymal Stem Cells in Allergic Rhinitis Mouse Model

Park

Kim

et al. 2021

Am J Rhinol�Allergy

Self Cite

View full text Add to dashboard Cite

Background Low-level light therapy (LLLT) is widely used for the photobiomodulation of cell behavior. Recent studies have shown that LLLT affects the proliferation and migration of various types of mesenchymal stem cells (MSCs). However, there is a lack of studies investigating the effect of LLT on enhancing the immunomodulatory properties of tonsil-derived MSCs (T-MSCs). Objective The aim of this study was to investigate the immunomodulatory effects of conditioned media from T-MSCs (T-MSCs-CM) treated with LLLT in allergic inflammation. Methods We isolated T-MSCs from human palatine tonsils and evaluated the ingredients of T-MSCs-CM. The effect of T-MSCs-CM treated with LLLT was evaluated in a mouse model of allergic rhinitis (AR). We randomly divided the mice into four groups (negative control, positive control, T-MSCs-CM alone, and T-MSCs-CM treated with LLLT). To elucidate the therapeutic effect, we assessed rhinitis symptoms, serum immunoglobulin (Ig), the number of inflammatory cells, and cytokine expression. Results We identified increased expression of immunomodulatory factors, such as HGF, TGF-β, and PGE, in T-MSCs-CM treated with LLLT, compared to T-MSCs-CM without LLLT. Our animal study demonstrated reduced allergic symptoms and lower expression of total IgE and OVA-specific IgE in the LLLT-treated T-MSCs-CM group compared to the AR group and T-MSCs-CM alone. Moreover, we found that T-MSCs-CM treated with LLLT showed significantly decreased infiltration of eosinophils, neutrophils, and IL-17 cells in the nasal mucosa and reduced IL-4, IL-17, and IFN-γ expression in OVA-incubated splenocytes compared to the AR group. Conclusions The present study suggests that T-MSCs-CM treated with LLLT may provide an improved therapeutic effect against nasal allergic inflammation than T-MSCs-CM alone.

show abstract

The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model

Cited by 10 publications

References 35 publications

Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology

Achievements and Challenges in Transplantation of Mesenchymal Stem Cells in Otorhinolaryngology

IDO and CD40 May Be Key Molecules for Immunomodulatory Capacity of the Primed Tonsil-Derived Mesenchymal Stem Cells

Increased Anti-Allergic Effects of Secretome of Low-Level Light Treated Tonsil-Derived Mesenchymal Stem Cells in Allergic Rhinitis Mouse Model

Contact Info

Product

Resources

About