Immunomodulatory effect of tea saponin in immune T-cells and T-lymphoma cells<i>via</i>regulation of Th1, Th2 immune response and MAPK/ERK2 signaling pathway

Bhardwaj, Jyoti; Chaudhary, Narendra; Seo, Hyo-Jin; Kim, Min-Yong; Shin, Tai-Sun; Kim, Jong-Deog

doi:10.3109/08923973.2014.909849

Cited by 24 publications

(13 citation statements)

References 37 publications

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“…The saponins have been found to have numerous pharmacological effects including anti-fungal [ 29 ], anti-viral [ 30 ], anti-inflammatory [ 31 ], anti-hyperlipidemic [ 32 ], immunomodulatory [ 33 ] and anti-tumor activities [ 17 , 19 ]. Among these, TSE 1 was proved to have higher bioactivity than other saponin monomers present in tea seeds [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

et al. 2018

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Ovarian cancer has the highest mortality rate of all gynecological malignancies and the five-year death rate of patients has remained high in the past five decades. Recently, with the rise of cancer stem cells (CSCs) theory, an increasing amount of research has suggested that CSCs give rise to tumor recurrence and metastasis. Theasaponin E1 (TSE1), which was isolated from green tea (Camellia sinensis) seeds, has been proposed to be an effective compound for tumor treatment. However, studies on whether TSE1 takes effect through CSCs have rarely been reported. In this paper, ALDH-positive (ALDH+) ovarian cancer stem-like cells from two platinum-resistant ovarian cancer cell lines A2780/CP70 and OVCAR-3 were used to study the anti-proliferation effect of TSE1 on CSCs. The ALDH+ cells showed significantly stronger sphere forming vitality and stronger cell migration capability. In addition, the stemness marker proteins CD44, Oct-4, Nanog, as well as Bcl-2 and MMP-9 expression levels of ALDH+ cells were upregulated compared with the original tumor cells, indicating that they have certain stem cell characteristics. At the same time, the results showed that TSE1 could inhibit cell proliferation and suspension sphere formation in ALDH+ cells. Our data suggests that TSE1 as a natural compound has the potential to reduce human ovarian cancer mortality. However, more research is still needed to find out the molecular mechanism of TSE1-mediated inhibition of ALDH+ cells and possible drug applications on the disease.

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Section: Discussionmentioning

confidence: 99%

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

et al. 2018

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“…TS was isolated from Green tea ( Camellia sinensis (L.) O. Kuntze) seeds, which were freshly harvested from the National germplasm Hangzhou tea tree nursery, Hangzhou, China, and modified by previously-established methods [ 36 ]. Briefly, the dried tea seeds were pulverized and subsequently extracted with 70% MeOH (1:15 w / v ) under reflux for 5 h at 70 °C.…”

Section: Methodsmentioning

confidence: 99%

“…TS has been demonstrated as being an excellent bio-surfactant with numerous pharmacological activities. TS has been documented as having gastroprotective [ 33 ], anti-fungal [ 34 ], anti-viral [ 25 ], anti-oxidative [ 24 ], anti-obesity [ 35 ], anti-inflammatory [ 24 , 28 ], anti-hyperglycemic [ 27 ], immunomodulatory [ 36 ], and anti-tumor [ 35 , 37 , 38 ] tendencies. However, unlike other phenolic compounds, EGCG [ 39 , 40 , 41 ] and TFs [ 37 , 42 ] separated from tea plants, TS has not been reported as having pharmacological influences on human ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Total Triterpenoid Saponins Isolated from the Seeds of the Tea Plant (Camellia sinensis) on Human Ovarian Cancer Cells

Jia

Gao

et al. 2017

Molecules

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Ovarian cancer is regarded as one of the most severe malignancies for women in the world. Death rates have remained steady over the past five decades, due to the undeniable inefficiency of the current treatment in preventing its recurrence and death. The development of new effective alternative agents for ovarian cancer treatment is becoming increasingly critical. Tea saponins (TS) are triterpenoidsaponins composed of sapogenins, glycosides, and organic acids, which possess a variety of pharmacological activities, and have shown promise in the anti-cancer field. Through cell CellTiter 96® Aqueous One Solution Cell Proliferation assay (MTS) assay, colony formation, Hoechst 33342 staining assay, caspase-3/7 activities, flow cytometry for apoptosis analysis, and Western blot, we observed that TS isolated from the seeds of tea plants, Camellia sinensis, exhibited strong anti-proliferation inhibitory effects on OVCAR-3 and A2780/CP70 ovarian cancer cell lines. Our results indicate that TS may selectivity inhibit human ovarian cancer cells by mediating apoptosis through the extrinsic pathway, and initiating anti-angiogenesis via decreased VEGF protein levels in a HIF-1α-dependent pathway. Our data suggests that, in the future, TS could be incorporated into a potential therapeutic agent against human ovarian cancer.

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“…A previous study has also shown that TSP can improve the permeability of capillaries in hypertensive rats (Sagesaka-Mitane et al, 1996). The lower SCC found after supplementation of 20 and 30 g/d of TSP confirmed its function in immunomodulatory effects (Bhardwaj et al, 2014). Previous studies also found that milk SCC is related to the antioxidant status of the body (Suriyasathaporn et al, 2006).…”

Section: Bloodmentioning

confidence: 78%

Effect of tea saponins on milk performance, milk fatty acids, and immune function in dairy cow

Wang

Yan

Zhao

et al. 2017

Journal of Dairy Science

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This study investigated the effects of tea saponins (TSP) on milk performance, milk fatty acids, and blood immune function in dairy cows. A total of 20 early-lactation Holstein cows (days in milk = 66.4 ± 16.8 d; parity = 1.75 ± 0.91; and milk yield = 36.3 ± 7.32 kg/d; mean ± standard deviation) were randomly divided into 4 homogeneous treatment groups, with TSP added at 0, 20, 30, and 40 g/d per head, respectively. All cows had 2 wk of adaptation and 6 wk of treatments. Feed, milk, and blood were sampled and analyzed weekly. At the end of the experimental period (wk 6), the dry matter intake and yields of energy-corrected milk, milk, and milk protein, fat, and lactose in the cows fed TSP showed a quadratic response, with the lowest values in cows fed TSP at 40 g/d. The milk fat content of cows fed TSP increased linearly. Significant interactions for treatment by week were found in milk C16:1 cis-9 and C18:1 cis-9, with the highest values at wk 2, 3, and 4 in the cows fed TSP at 40 g/d. The levels declined quickly after 4 wk of feeding to values similar to those for other TSP treatments and the control at wk 5 and 6. Plasma malondialdehyde concentration decreased as the supplement level of TSP increased. The concentration of superoxide dismutase increased as the supplement level of TSP increased. The plasma concentration of tumor necrosis factor-α increased as the supplement level of TSP increased. In summary, this study showed that an intermediate dose of TSP (20 and 30 g/d) had no significant effect on feed intake, but the supplementation of 40 g/d TSP decreased feed intake, resulting in a lower milk yield. The energy-corrected milk of cows fed 40 g/d TSP declined at first but increased after 3 wk of feeding, indicating the potential adaptation to high doses of TSP supplements in dairy cows. The supplementation of TSP could reduce oxidative stress in cows and improve the immunity of dairy cows during 6 wk of feeding.

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Immunomodulatory effect of tea saponin in immune T-cells and T-lymphoma cellsviaregulation of Th1, Th2 immune response and MAPK/ERK2 signaling pathway

Cited by 24 publications

References 37 publications

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

Inhibitory Effects of Total Triterpenoid Saponins Isolated from the Seeds of the Tea Plant (Camellia sinensis) on Human Ovarian Cancer Cells

Effect of tea saponins on milk performance, milk fatty acids, and immune function in dairy cow

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