Inhibitory Effects of Total Triterpenoid Saponins Isolated from the Seeds of the Tea Plant (Camellia sinensis) on Human Ovarian Cancer Cells

Jia, Ling-Yan; Wu, Xuejin; Gao, Ying; Rankin, Gary O.; Pigliacampi, Alexa; Bucur, Heather; Li, Bo; Chen, Yi Charlie

doi:10.3390/molecules22101649

Cited by 23 publications

(21 citation statements)

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“…The saponins have been found to have numerous pharmacological effects including anti-fungal [ 29 ], anti-viral [ 30 ], anti-inflammatory [ 31 ], anti-hyperlipidemic [ 32 ], immunomodulatory [ 33 ] and anti-tumor activities [ 17 , 19 ]. Among these, TSE 1 was proved to have higher bioactivity than other saponin monomers present in tea seeds [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…While the IC 50 value of TSE 1 for normal ovarian surface epithelial cell line IOSE-364 was 23.82 µM, TSE 1 showed a much lower cytotoxic effect against normal ovarian epithelial cells. In addition, our published data showed that the IC 50 values of cisplatin, which is the most effective and widely used chemotherapeutic agent, were 11.91 µg/mL (35.71 µM) for A2780/CP70 and 10.10 µg/mL (30.30 µM) for OVCAR-3, respectively [ 19 ]. All data taken together, we indicated that TSE 1 exhibited higher inhibitory effects compared to cisplatin.…”

Section: Discussionmentioning

confidence: 99%

“…Cell growth inhibition was determined by using MTS assay as described as before [ 19 ]. Sphere cells (3-weeks) were seeded into 96-well ultra-low adherent plates at a density of 2000 cells/well in complete medium for 24 h. Then, cells were treated with various concentrations of TSE 1 (0 to 40 μg/mL) for 24, 48 and 72 h at 37 °C in a humidified 5% CO 2 incubator.…”

Section: Methodsmentioning

confidence: 99%

“…It could also suppress the human tumor cell lines K562 and HL60 [ 18 ]. Our previous study showed that the total saponins from C. sinensis seeds exhibit potent growth inhibitory effect on two cisplatin-resistant ovarian cancer cell lines A2780/CP70 and OVCAR-3 [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

et al. 2018

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Ovarian cancer has the highest mortality rate of all gynecological malignancies and the five-year death rate of patients has remained high in the past five decades. Recently, with the rise of cancer stem cells (CSCs) theory, an increasing amount of research has suggested that CSCs give rise to tumor recurrence and metastasis. Theasaponin E1 (TSE1), which was isolated from green tea (Camellia sinensis) seeds, has been proposed to be an effective compound for tumor treatment. However, studies on whether TSE1 takes effect through CSCs have rarely been reported. In this paper, ALDH-positive (ALDH+) ovarian cancer stem-like cells from two platinum-resistant ovarian cancer cell lines A2780/CP70 and OVCAR-3 were used to study the anti-proliferation effect of TSE1 on CSCs. The ALDH+ cells showed significantly stronger sphere forming vitality and stronger cell migration capability. In addition, the stemness marker proteins CD44, Oct-4, Nanog, as well as Bcl-2 and MMP-9 expression levels of ALDH+ cells were upregulated compared with the original tumor cells, indicating that they have certain stem cell characteristics. At the same time, the results showed that TSE1 could inhibit cell proliferation and suspension sphere formation in ALDH+ cells. Our data suggests that TSE1 as a natural compound has the potential to reduce human ovarian cancer mortality. However, more research is still needed to find out the molecular mechanism of TSE1-mediated inhibition of ALDH+ cells and possible drug applications on the disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

et al. 2018

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“…Furthermore, the protective effects of C. sinensis were demonstrated in different types of cancers such as breast, ovarian, prostate and pancreatic cancers. The importance of anti-cancer activity of tea was highlighted (6)(7)(8)(9) In the current study, the effects of the extract from C. sinensis on cytotoxicity, proliferation, apoptosis and oxidative stress status were investigated in rat insulinoma INS-1 cells.…”

Section: Introductionmentioning

confidence: 98%

The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells

Kaçar¹,

Ersöz²,

SEZEKLER³

et al. 2019

Bezmialem Science

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Objective: Green tea (Camellia sinensis) is one of the most consumed beverages in the world. C. sinensis consumption may prevent tumor and other diseases. Insulinomas are pancreatic islet cell tumors. In present study, the effects of the extract from C. sinensis were investigated in rat insulinoma INS-1 cells. Methods: C. sinensis leaves were collected in Rize, Turkey. The leaves were brewed in tap water for 20 and 40 minutes at 80°C. INS-1 cells were treated with different doses (1-5 mg/mL) of C. sinensis extract for 24 h. Cell viability, proliferation, death were examined. The malondialdehyde, glutathione and protein carbonyl levels were measured in INS-1 cells given C. sinensis extract. Results: The cell viability and the percentage of proliferating cell nuclear antigen immunopositive cells decreased, while the percentage of TUNEL positive cells increased in INS-1 cells treated with the extract from C. sinensis according to control group. While malondialdehyde and protein carbonyl levels in INS-1 cells treated with C. sinensis extract decreased, glutathione levels increased according to control group. Conclusion: The results indicate that the extracts from C. sinensis inhibited proliferation and caused apoptosis in INS-1 cell. C. sinensis may be natural agent for supporting the treatment of pancreatic tumors.

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Molecular mechanisms of action and chemosensitization of tumor cells in ovarian cancer by phytochemicals: A narrative review on pre‐clinical and clinical studies

Barboza

Pereira

Vasconcelos

et al. 2023

Phytotherapy Research

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Ovarian cancer is the second-leading cause of death among women with cancer of the genital tract. Currently, drugs derived from platinum and taxanes constitute the majority of ovarian cancer treatments. Patients undergoing this chemotherapy are susceptible to cumulative toxic effects and resistance to chemotherapy. Therefore, it is crucial to identify treatment options that are both more effective and better tolerated by patients. Phytochemicals in this context are plant-derived chemicals with antitumor activity that can be used as therapeutic or adjuvant agents in the treatment of ovarian cancer. Consequently, the purpose of this literature review is to demonstrate through existing pre-clinical and clinical trials the potential of phytochemicals in the treatment of ovarian cancer, the mechanisms of action involved, and to contribute to the development of new therapeutic options for ovarian cancer. For this review, the databases PubMed, Scopus, Science Direct, and ClinicalTrials.gov were queried between 2010 and 2022 using terms such as "ovarian cancer," "phytochemicals," "phenolic compounds," "terpenes," and "alkaloids." The present review summarized the possible molecular mechanisms of action by which phytochemicals, such as phenolic acids, flavonoids, diterpenes, triterpenes, saponins, and alkaloids, inhibit this type of cancer, specifically the ability of phytochemicals to induce cell growth regulation, apoptosis, oxidative stress reduction, anti-angiogenesis, and chemosensitization of tumors in ovarian cancer. As their action and cellular mechanism have already been demonstrated in several pre-clinical trials, the

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Inhibitory Effects of Total Triterpenoid Saponins Isolated from the Seeds of the Tea Plant (Camellia sinensis) on Human Ovarian Cancer Cells

Cited by 23 publications

References 53 publications

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

Anti-Proliferation Effect of Theasaponin E1 on the ALDH-Positive Ovarian Cancer Stem-Like Cells

The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells

Molecular mechanisms of action and chemosensitization of tumor cells in ovarian cancer by phytochemicals: A narrative review on pre‐clinical and clinical studies

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