Immunomodulatory activity on macrophage of a purified polysaccharide extracted from Laminaria japonica

Fang, Qing; Wang, Jingfei; Zha, Xue‐Qiang; Cui, Shao-Hua; Cao, Lei; Luo, Jian‐Ping

doi:10.1016/j.carbpol.2015.07.070

Cited by 134 publications

(58 citation statements)

References 44 publications

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“…[46] Recently, various natural polysaccharides, such as polysaccharides from Laminaria japonica , Polyporus umbellatus , and Astragalus membranaceus have been reported to trigger the activation of macrophages via the TLR4 receptor. [47–49] We hypothesized that TLR4 might be a candidate receptor/binding site for WBP. To determine the underlying signaling mechanism by which WBP promotes p38 MAPK, cells were stimulated with 100 µg ml –1 WBP for various lengths of time (5, 10, 30, and 60 min) and analyzed by Western blot.…”

Section: Resultsmentioning

confidence: 99%

Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice

Shen

Wang

You

et al. 2017

Food & Nutrition Research

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Wheat bran-derived polysaccharides have attracted particular attention due to their immunomodulatory effects. However, the molecular mechanisms underlying their functions are poorly understood. The current study was designed to examine the effect of wheat bran polysaccharide (WBP) on RAW 264.7 cells and the underlying signaling pathways, which have not been explored. In addition, we also investigated the immuno-enhancement effects of WBP on cyclophosphamide (CTX)-induced immunosuppression in mice. WBP significantly increased the concentrations of intracellular nitric oxide (NO) and cytokines such as prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) in RAW 264.7 cells. The result of RT-PCR analysis indicated that WBP also enhanced inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α expression. Further analyses demonstrated that WBP rapidly activated phosphorylated p38 mitogen-activated protein kinase (MAPK) and the transcriptional activities of activator protein-1 (AP-1) and nuclear factor (NF)-κB via toll-like receptor 4 (TLR4). Furthermore, in vivo experiments revealed that WBP increased the spleen and thymus indices significantly, and markedly promoted the production of the serum cytokines IL-2 and IFN-γ in CTX-induced immunosuppressed mice. Taken together, these results suggest that WBP can improve immunity by enhancing immune function, and could be explored as a potential immunomodulatory agent in functional food.

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Section: Resultsmentioning

confidence: 99%

Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice

Shen

Wang

You

et al. 2017

Food & Nutrition Research

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“…Over the past decades, L. japonica has drawn the attention of chemists and pharmacologists on account of the abundance of functional compounds and their biological properties [4]. Many studies have recently suggested that polysaccharides were the main active components in L. japonica [5]. Polysaccharides, one of the main classes of bioactive substances from fungi, algae, and higher plants, have been demonstrated to exhibit a wide range of pharmacological activities, including broad immunomodulatory and antitumor effects [6,7].…”

Section: Introductionmentioning

confidence: 99%

Laminarin enhances the activity of natural killer cells in immunosuppressed mice

Zhu

Jian

et al. 2019

cejoi

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The aim of the study was to investigate the effects of laminarin on natural killer (NK) cell cytotoxicity of immunosuppressive mice and its mechanism. Cyclophosphamide (cy) was used to make an immunosuppressive model of mice. The mice of two groups were given interventions by gavage with laminarin 500 mg/kg and 1000 mg/kg every day for 10 days. MACS was adopted to isolate spleen NK cells, and cytotoxicity of NK cells and IL-12, IFN-γ level in serum were detected in vivo. Cytotoxicity of NK92-MI cells, activating receptors (NKp30, NKp44, NKp46 and NKG2D) and perforin and granzyme B expression were detected in vitro. Compared to the normal control group, the cytotoxicity of NK cells, IL-12 and IFN-γ level in serum in the cy model group were all reduced significantly (p < 0.01). Compared to the cy model group, laminarin increased the cytotoxicity of NK cells, IL-12 and IFN-γ levels in serum significantly (p < 0.05). In vitro, laminarin increased the cytotoxicity, NKp30 and NKG2D, perforin and granzyme B expressions of NK92-MI cells (p < 0.01). This research showed that laminarin can promote NK cell cytotoxicity in immunosuppressive mice by increasing the levels of IL-12 and IFN-γ in serum and expressions of NKp30 and NKG2D, perforin and granzyme B.

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“…The immunity plays as a body protective role to identify and eliminate the exogenous harmful substances from living organisms (Fang and others ). Among those immune responses, the activation of macrophages’ secretion is a significant mechanism, which macrophages are considered as the target immunocytes for fighting infection and inflammation (Cheng and others ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Immunomodulatory Effects of Fresh Garlic and Black Garlic Polysaccharides on RAW 264.7 Macrophages

Yan

et al. 2017

Journal of Food Science

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Garlic has a long history to be used for medicine and food purposes. Black garlic, the fermented product of fresh garlic, is considered with better biological activities, such as antioxidant activity, and is developed as an increasingly popular functional food. Polysaccharides are the major components of fresh and black garlic, and immunomodulatory activity is one major pharmacological effect of polysaccharides. Therefore, chemical characteristics and immunomodulatory effects of polysaccharides from fresh and black garlic are investigated and compared in vitro for the 1st time, in order to reveal their molecular and pharmacological differences. It is demonstrated that the molecular weights of polysaccharides from the 2 sources and molar ratios of monosaccharides after acid hydrolysis are greatly variant. The effects of polysaccharides from 2 sources on RAW 264.7 macrophages functions, including promotion of phagocytosis, release of NO, and expressions of several immune-related cytokines (including interleukin [IL]-6, IL-10, tumor necrosis factor alpha, and interferon gamma), were different from each other. The results indicated that fresh garlic polysaccharide exhibited stronger immunomodulatory activities than that of black garlic. Moreover, it is revealed that fructan might be the bioactive component in garlic and it is indicated that during the fermentation treatment, fructan constituents of garlic has degraded, and basically no immunomodulatory effect can be found in black garlic polysaccharides.

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Immunomodulatory activity on macrophage of a purified polysaccharide extracted from Laminaria japonica

Cited by 134 publications

References 44 publications

Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice

Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice

Laminarin enhances the activity of natural killer cells in immunosuppressed mice

Comparison of Immunomodulatory Effects of Fresh Garlic and Black Garlic Polysaccharides on RAW 264.7 Macrophages

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