Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential

Gautam, Manish; Saha, Santanu; Bani, Sarang; Kaul, Anpurna; Mishra, Sanjay; Patil, Dada; Satti, N. K.; Suri, K. A.; Gairola, Sunil; Suresh, K.; Jadhav, Suresh; Qazi, Ghulam N.; Patwardhan, Bhushan

doi:10.1016/j.jep.2008.10.028

Cited by 175 publications

(78 citation statements)

References 31 publications

(28 reference statements)

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“…This country is well recognized for its heritage of the world's most ancient traditional system of medicine, Ayurveda (Gautam et al 2009). One of the best approaches in the search for anticancer agent from plant resource is the selection of plant species based upon ethno-medical leads.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

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Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor activity. Extract/fractions were prepared and in vitro cytotoxicity was assessed using SRB assay. Most effective fractions were subjected to fluorescence microscopy based acridine orange/ethidium bromide (AO/EB) and Hoechst 33342 staining to determine apoptosis induction and DNA fragmentation assay. Comet and micronuclei assay were performed to assess genotoxicity. Cell cycle analysis was also performed. In vivo anti-tumor potential was evaluated by Ehrlich ascites carcinoma (EAC) model in mice. The alcoholic stem bark extract of M. elengi along with four fractions showed potential in vitro cytotoxicity in SRB assay. Of these, dichloromethane and ethyl acetate fractions were selected for further studies. The fractions revealed apoptosis inducing potential in AO/EB and Hoechst 33342 staining, which was further confirmed by DNA fragmentation assay. Genotoxic potential was revealed by comet and micronuclei assay. Fractions also exhibited specific cell cycle inhibition in G 0 /G 1 phase. In EAC model, ethyl acetate fraction along with the standard (cisplatin) effectively reduced the increase in body weight compared to control and improved mean survival time. Both fractions were able to restore the altered hematological and biochemical parameters. Hence, M. elengi stem bark may be a possible therapeutic candidate having cytotoxic and anti-tumor potential.

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Section: Introductionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

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“…In Ayurveda, Asparagus racemosus has been used extensively as an adaptogen to increase the nonspecific resistance of organisms against a variety of stresses (Visavadiya et al, 2009;Wiboonpun et al, 2004). Besides use in the treatment of diarrhoea and dysentery, the plant also has antioxidant, immunestimulant, anti-dyspepsia and anti-tussive effects (Visavadiya et al, 2009;Wiboonpun et al, 2004;Kamat et al, 2000;Ojha et al, 2010;Gautam et al, 2009;Mandal et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Investigation of cytotoxicity and in-vitro antioxidant activity of Asparagus racemosus root extract

et al. 2012

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This attempt is made to address the phytoconstituents, free radical scavenging activity and brine shrimp lethality bioassay of five different extracts of Asparagus racemosus roots. Preliminary phytochemical examination of the crude extracts of Asparagus racemosus root disclosed the existence of different sort of chemical groups such as flavonoids, tannin, saponin, alkaloids, carbohydrate. The root displayed significant DPPH free radical scavenging activity with highest IC50 value showed by ethanol extract with a value of 78.15 µg/ml followed by methanol and petroleum ether having value of 106.44 µg/ml and 273.31 µg/ml respectively as opposed to that of the scavenging effects of ascorbic acid and BHT of 5.698 µg/ml and 8.816 µg/ml respectively. The highest reducing power was showed by ethanol extract followed by methanol and petroleum ether as opposed to that of the reducing potential of ascorbic acid and BHT. The fractions represented good cupric reducing capacity with increasing concentration taking ethanol extract in the top position. The ethanol extract yielded 108.78±2.77 mg/gm gallic acid equivalent phenolic content and methanol sub-fraction yielded 164.77±1.73 mg/gm quercetin equivalent flavonoid content that was highest among five extracts. Ethanol extract of Asparagus racemosus was found to possess the highest total antioxidant capacity (639.925±64.78) mg/gm followed by methanol (616.92±53.88) mg/gm and petroleum ether (469.17±52.95) mg/gm ascorbic acid equivalent respectively. In brine shrimp lethality bioassay, LC50 values for ethanol, methanol, petroleum ether, n-hexane and chloroform were found to be 0.674 µg/ml, 0.719 µg/ml, 0.984 µg/ml, 2.157µg/ml and 1.514 µg/ml respectively. N-hexane and chloroform extract showed least activity in all the measures. The results suggest that Asparagus racemosus is a valuable source of antioxidant and has significant cytotoxic activity hence could eliminate many diseases related to free radical.DOI: http://dx.doi.org/10.3329/icpj.v1i9.11615 International Current Pharmaceutical Journal 2012, 1(9): 250-257

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“…There is an evidence to suggest that delayed type hypersensitivity reaction is important in host defense against parasites and bacteria that can live and proliferate intracellularly. An increase in delayed type hypersensitivity response indicates that plant extract and fraction have a stimulatory effect on lymphocytes and accessory cell types required for the expression of reaction response and B cell activation [13,14,16] .…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of butanol fraction of Gentiana olivieri Griseb. on Balb/C mice

Singh¹,

Yadav²,

Noolvi³

2012

Asian Pacific Journal of Tropical Biomedicine

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Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential

Cited by 175 publications

References 31 publications

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

Investigation of cytotoxicity and in-vitro antioxidant activity of Asparagus racemosus root extract

Immunomodulatory activity of butanol fraction of Gentiana olivieri Griseb. on Balb/C mice

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