Immunomagnetic separation of Salmonella with tailored magnetic micro and nanocarriers. A comparative study

Brandão, Delfina; Liébana, Susana; Campoy, Susana; Alegret, Salvador; Pividori, María Isabel

doi:10.1016/j.talanta.2015.05.035

Cited by 45 publications

(29 citation statements)

References 29 publications

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“…MNPs have larger surface-to-volume ratio providing more chemical reactive sites for the attachment of higher amounts of smaller size (<1 μm) biomolecules at their surface. Despite this improved reactivity, similar or slightly better analytical performance with MNPs compared to MMPs has been reported in standard solutions [96]. However lower efficiency for immunomagnetic separation was achieved in complex samples (such as whole milk) using MNPs attributable to these nanoparticles might be more affected by matrix effect [5].…”

Section: General Conclusion and Future Prospectsmentioning

confidence: 94%

“…However lower efficiency for immunomagnetic separation was achieved in complex samples (such as whole milk) using MNPs attributable to these nanoparticles might be more affected by matrix effect [5]. Moreover, MNPs are easily aggregated and required longer times for magnetic actuation [96]. The wide commercial availability of MMPs modified with various functional groups greatly facilitates the design of many different approaches test depending on the particular application.…”

Section: General Conclusion and Future Prospectsmentioning

confidence: 99%

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Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

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Ultrasensitive biosensing is currently a growing demand that has led to the development of numerous strategies for signal amplification. In this context, the unique properties of magnetic particles; both of nano- and micro-size dimensions; have proved to be promising materials to be coupled with disposable electrodes for the design of cost-effective electrochemical affinity biosensing platforms. This review addresses, through discussion of selected examples, the way that nano- and micro-magnetic particles (MNPs and MMPs; respectively) have contributed significantly to the development of electrochemical affinity biosensors, including immuno-, DNA, aptamer and other affinity modes. Different aspects such as type of magnetic particles, assay formats, detection techniques, sensitivity, applicability and other relevant characteristics are discussed. Research opportunities and future development trends in this field are also considered.

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Section: General Conclusion and Future Prospectsmentioning

confidence: 94%

Section: General Conclusion and Future Prospectsmentioning

confidence: 99%

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

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“…MNPs consist of iron, cobalt, and their oxides or alloys that exhibit superparamagnetic properties but are smaller than microbeads. MNPs have capture efficiencies comparable to those of their microsize counterparts and, in some cases, slightly higher detection sensitivity (8,67). Magnetic microbeads usually contain an amorphous polymer material (e.g., polystyrene) to support the magnetic components, which have pronounced inherent background fluorescence.…”

Section: Nanomaterial-assisted Sample Preparationmentioning

confidence: 99%

“…The nanostructure complex can then be used for both capture and detection without the necessity to form sandwich structures, which has been proven useful in SERS (121) and electrochemical (80,94) sensors. One potential disadvantage, however, is the higher susceptibility of MNPs to matrix interference and the tendency of MNPs to agglomerate in food matrices (8). Smaller (,100 nm) MNPs require a much higher gradient and strength magnetic field and hence prolonged magnetic separation period (usually hours) for full recovery (67).…”

Section: Nanomaterial-assisted Sample Preparationmentioning

confidence: 99%

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen

Park

2016

Journal of Food Protection

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Bacterial pathogens are one of the leading causes of food safety incidents and product recalls worldwide. Timely detection and identification of microbial contamination in agricultural and food products is crucial for disease prevention and outbreak investigation. In efforts to improve and/or replace time-consuming and laborious "gold standards" for pathogen detection, numerous alternative rapid methods have been proposed in the past 15 years, with a trend toward incorporating nanotechnology and nanomaterials in food pathogen detection. This article is a review of the use of nanotechnology in various detection and sample preparation techniques and advancements in nanotechnology applications in food matrices. Some practical considerations in nanobioassay design are discussed, and the gaps between research status quo and market demands are identified.

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“…Further improvement of the sensitivity can be achieved by the use of suitable labels . In case of amperometric detection, LODs are typically around 10 2 CFU mL −1 with analysis times ranging from 1 to 3 h . The sensitivity can be further enhanced e. g. by the use of magnetic microparticles, as reviewed recently .…”

Section: Introductionmentioning

confidence: 99%

Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius

et al. 2019

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European foulbrood (EFB) is a honeybee larvae disease caused by a bacterium Melissococcus plutonius. An amperometric immunosensor based on a sandwich assay was developed for rapid point‐of‐care detection of this pathogen. An in‐house made anti‐Melissococcus antibody was immobilized to a gold surface of a screen‐printed sensor via self‐assembled monolayer of cysteamine activated with glutaraldehyde. The direct impedimetric detection of captured microbial cells was tested, however, a better performance was obtained after the formation of sandwich with the peroxidase‐labeled antibody in the amperometric mode. The label‐free assay was limited by higher non‐specific binding. The limit of detection of the immunosensor was 6.6×104 CFU mL−1 (colony‐forming units) with wide linear range between 105 CFU mL−1 and 109 CFU mL−1. The whole analysis was completed within 2 h, which is shorter compared to common laboratory diagnostic tools, such as enzyme‐linked immunosorbent assay or polymerase chain reaction. Furthermore, atomic force microscopy was used for confirmation of the bacteria presence on the electrode surface. The developed immunosensor was successfully employed in the analysis of real samples of honeybees and larvae. The achieved results demonstrate the potential of the amperometric immunosensor for practical in‐field diagnosis of EFB, which can prevent infection spreading and connected losses of honeybee colonies.

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Immunomagnetic separation of Salmonella with tailored magnetic micro and nanocarriers. A comparative study

Cited by 45 publications

References 29 publications

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius

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