Immunomagnetic separation combined with RT-qPCR for evaluating the effect of disinfectant treatments against norovirus on food contact surfaces

Lee, Hee-Min; Yang, Jaesung; Yoon, So‐Ra; Lee, Jae Yong; Kim, Su-Ji; Lee, Hae-Won; Kim, Sung Hyun; Ha, Ji‐Hyoung

doi:10.1016/j.lwt.2018.06.041

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…These results did not show contamination; consequently, it demonstrated the specificity of the technique as nonspecific binding was not found. Our results are corroborated by other studies that have also demonstrated important findings on the capacity of IMS to concentrate viruses and on the increase of sensibility when combined with a molecular approach such as PCR or qPCR [29,31,41,42]. A result that must be noted is the increase of efficiency in detecting samples with low amounts of HAdV genomic copies when these were concentrated by IMS.…”

Section: Standardization Of Ims Parameterssupporting

confidence: 89%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources.

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Section: Standardization Of Ims Parameterssupporting

confidence: 89%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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“…The spread of COVID-19 among human populaces might be identified too late, and the virus can only be detected when an enormous scale of human testing is accessible, or when clinical COVID-19 cases are accounted for by health experts, because the carriers are believed to exhibit no symptoms, or few non-specific symptoms [15]. Therefore, there have been investigations into the virus circulation among developed countries and emerging economies, including China, Netherlands, and Australia using stool samples [16]. The WHO (2020) reported that as of March 3, 2020, only two studies had been able to detect the viral RNA fragments of COVID-19 in the faucal matter of infected patients and that the transmission of the virus through faucal matter was low.…”

Section: Current State Of Covid-19 In Wastewatermentioning

confidence: 99%

Fate of COVID-19 Occurrences in Wastewater Systems: Emerging Detection and Treatment Technologies—A Review

Tetteh

Amankwa

Armah

et al. 2020

Water

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The coronavirus (COVID-19) pandemic is currently posing a significant threat to the world’s public health and social-economic growth. Despite the rigorous international lockdown and quarantine efforts, the rate of COVID-19 infectious cases remains exceptionally high. Notwithstanding, the end route of COVID-19, together with emerging contaminants’ (antibiotics, pharmaceuticals, nanoplastics, pesticide, etc.) occurrence in wastewater treatment plants (WWTPs), poses a great challenge in wastewater settings. Therefore, this paper seeks to review an inter-disciplinary and technological approach as a roadmap for the water and wastewater settings to help fight COVID-19 and future waves of pandemics. This study explored wastewater–based epidemiology (WBE) potential for detecting SARS-CoV-2 and its metabolites in wastewater settings. Furthermore, the prospects of integrating innovative and robust technologies such as magnetic nanotechnology, advanced oxidation process, biosensors, and membrane bioreactors into the WWTPs to augment the risk of COVID-19’s environmental impacts and improve water quality are discussed. In terms of the diagnostics of COVID-19, potential biosensors such as sample–answer chip-, paper- and nanomaterials-based biosensors are highlighted. In conclusion, sewage treatment systems, together with magnetic biosensor diagnostics and WBE, could be a possible way to keep a surveillance on the outbreak of COVID-19 in communities around the globe, thereby identifying hotspots and curbing the diagnostic costs of testing. Photocatalysis prospects are high to inactivate coronavirus, and therefore a focus on safe nanotechnology and bioengineering should be encouraged.

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Development and evaluation of a rapid immunomagnetic extraction for effective detection of zearalenone in agricultural products

et al. 2020

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Immunomagnetic separation combined with RT-qPCR for evaluating the effect of disinfectant treatments against norovirus on food contact surfaces

Cited by 6 publications

References 20 publications

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Fate of COVID-19 Occurrences in Wastewater Systems: Emerging Detection and Treatment Technologies—A Review

Development and evaluation of a rapid immunomagnetic extraction for effective detection of zearalenone in agricultural products

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