Immunomagnetic separation and PCR detection of<i>Listeria monocytogenes</i>in turkey meat and antibiotic resistance of the isolates

Ormanci, Fatma Seda Bilir; Erol, İrfan; Ayaz, Naim Deniz; İşeri, Özlem Darcansoy; Sariguzel, D.

doi:10.1080/00071660802298328

Cited by 30 publications

(27 citation statements)

References 25 publications

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“…After assessing all the information from the recovered publications, seventy-eight primary studies [6,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

Gonçalves-Tenório

Silva

Rodrigues

et al. 2018

Foods

111

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The objective of this study was to investigate and summarize the levels of incidence of Salmonella spp., Listeria monocytogenes, Staphylococcus aureus and Campylobacter spp. in poultry meat commercialized in Europe. After systematic review, incidence data and study characteristics were extracted from 78 studies conducted in 21 European countries. Pooled prevalence values from 203 extracted observations were estimated from random-effects meta-analysis models adjusted by pathogen, poultry type, sampling stage, cold preservation type, meat cutting type and packaging status. The results suggest that S. aureus is the main pathogen detected in poultry meat (38.5%; 95% CI: 25.4–53.4), followed by Campylobacter spp. (33.3%; 95% CI: 22.3–46.4%), while L. monocytogenes and Salmonella spp. present lower prevalence (19.3%; 95% CI: 14.4–25.3% and 7.10%; 95% CI: 4.60–10.8%, respectively). Despite the differences in prevalence, all pathogens were found in chicken and other poultry meats, at both end-processing step and retail level, in packed and unpacked products and in several meat cutting types. Prevalence data on cold preservation products also revealed that chilling and freezing can reduce the proliferation of pathogens but might not be able to inactivate them. The results of this meta-analysis highlight that further risk management strategies are needed to reduce pathogen incidence in poultry meat throughout the entire food chain across Europe, in particular for S. aureus and Campylobacter spp.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

Gonçalves-Tenório

Silva

Rodrigues

et al. 2018

Foods

111

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“…Antibodies are routinely used as affinity ligands to separate and concentrate the target analyte from sample matrices using paramagnetic beads (PMBs) [31-34] and also as recognition or reporter molecules on immunoassay platforms [31,35,36]. The PMB-captured cells may be presumptively identified by plating them on selective or differential media [37], or their identity may be confirmed by PCR [38,39], flow cytometry [40], or cytotoxicity assay [41]. The use of a biosensor to detect cells captured by immunomagnetic separation (IMS) is an attractive approach due to increased speed, accuracy, and detection of a low number of targets [34,42,43].…”

Section: Introductionmentioning

confidence: 99%

Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii

et al. 2012

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BackgroundImmunomagnetic separation (IMS) and immunoassays are widely used for pathogen detection. However, novel technology platforms with highly selective antibodies are essential to improve detection sensitivity, specificity and performance. In this study, monoclonal antibodies (MAbs) against Internalin A (InlA) and p30 were generated and used on paramagnetic beads of varying diameters for concentration, as well as on fiber-optic sensor for detection.ResultsAnti-InlA MAb-2D12 (IgG2a subclass) was specific for Listeria monocytogenes and L. ivanovii, and p30-specific MAb-3F8 (IgM) was specific for the genus Listeria. At all bacterial concentrations (103–108 CFU/mL) tested in the IMS assay; the 1-μm diameter MyOne beads had significantly higher capture efficiency (P < 0.05) than the 2.8-μm diameter M-280 beads with both antibodies. The highest capture efficiency for MyOne-2D12 (49.2% for 105 CFU/mL) was significantly higher (P < 0.05) than that of MyOne-3F8 (16.6 %) and Dynabeads anti-Listeria antibody (9 %). Furthermore, capture efficiency for MyOne-2D12 was highly specific for L. monocytogenes and L. ivanovii. Subsequently, we captured L. monocytogenes by MyOne-2D12 and MyOne-3F8 from hotdogs inoculated with mono- or co-cultures of L. monocytogenes and L. innocua (10–40 CFU/g), enriched for 18 h and detected by fiber-optic sensor and confirmed by plating, light-scattering, and qPCR assays. The detection limit for L. monocytogenes and L. ivanovii by the fiber-optic immunosensor was 3 × 102 CFU/mL using MAb-2D12 as capture and reporter antibody. Selective media plating, light-scattering, and qPCR assays confirmed the IMS and fiber-optic results.ConclusionsIMS coupled with a fiber-optic sensor using anti-InlA MAb is highly specific for L. monocytogenes and L. ivanovii and enabled detection of these pathogens at low levels from buffer or food.

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“…In all other papers including this study, concerning the Japanese situation, no resistant isolates were reported. It is considered that ABPC-resistant isolates may be rare in Japan, compared to Turkey [3,4,23] or the U.S. [15,18], although the reasons are unclear.…”

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confidence: 99%

Antimicrobial Susceptibilities of Listeria monocytogenes Isolated in Japan

Okada¹,

Okutani²,

Suzuki³

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The antimicrobial susceptibility of 201 Listeria monocytogenes isolates from foods, environments, animals and human patients in Japan was determined. All isolates were susceptible to ampicillin, the first choice of drug for listeriosis treatment, chloramphenicol, dihydrostreptomycin, erythromycin, enrofloxacin, gentamicin, kanamycin, lincomycin, nosiheptide, salinomycin, vancomycin, and virginiamycin. A human strain was resistant to oxytetracycline. The Minimum Inhibitory Concentration (MIC) for 50% of the strains and the MIC for 90% of the strains were comparable in all the isolates. This is the first investigation to compare antibiotic resistances between isolates from foods and isolates from human patients in Japan. The result showed that most of the isolates were susceptible to antibiotics used in this study.

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Immunomagnetic separation and PCR detection ofListeria monocytogenesin turkey meat and antibiotic resistance of the isolates

Cited by 30 publications

References 25 publications

Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii

Antimicrobial Susceptibilities of Listeria monocytogenes Isolated in Japan

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