Antimicrobial Susceptibilities of Listeria monocytogenes Isolated in Japan

Okada, Yumiko; Okutani, Akiko; Suzuki, Hodaka; Asakura, Hiroshi; Monden, Shuko; Nakama, Akiko; Matsumoto, Tsutomu; Igimi, Shizunobu

doi:10.1292/jvms.11-0051

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…In our previous study about the resistance against 16 types of antibiotic in 101 isolates from patients and 100 isolates from foods and environment, we found that only 1 isolate from patient was resistant to oxytetracyclin and tetracyclin (Okada, 2011). This result supports that the listerial resistant rates to antibiotics in Japan are at low level basically.…”

Section: Discussionsupporting

confidence: 60%

“…All strains were tested their susceptibilities against 8 types of antibiotics; ampicillin (ABPC; Wako Chemicals, Tokyo, Japan), chloramphenicol (CM; Wako), enrofloxacin (ERFX; Sigma, St. Louis, USA), erythromycin (EM; Sigma), gentamicin (GM; Wako), kanamycin (KM; Wako), penicillin (PN; Wako) and tetracycline (TC; Wako) by standard plate dilution method as in the previous study (Okada, 2011). All antibiotics were used in two-fold serial dilution at concentrations from 0.25 to 128µg/ml.…”

Section: Antibiotics Susceptibility Testmentioning

confidence: 99%

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Antimicrobial Susceptibilities of Listeria monocytogenes Isolated from the Imported and the Domestic Foods in Japan

Okada¹

2015

JFNS

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Abstract:In vitro antimicrobial susceptibility of Listeria monocytogenes isolated from the imported and the domestic foods in Japan was determined by plate dilution method. Eleven isolates from domestic meat, meat products, liver, seafood and environment, and 16 isolates from imported meat and meat products were examined their susceptibilities against ampicillin, chloramphenicol, enrofloxacin, erythromycin, gentamicin, kanamycin, penicillin and tetracycline. All of the isolates except the one isolate from domestic scallop were susceptible to all the antibiotics tested. Only 1 isolate showed resistance to kanamycin and gentamicin. The minimum inhibitory concentration (MIC) for 50% of the strains and the MIC for 90% of the strains were comparable between the imported and the domestic food origins. These results suggest there were less differences of antimicrobial susceptibility between the two origins of Listeria isolates.

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Section: Discussionsupporting

confidence: 60%

Section: Antibiotics Susceptibility Testmentioning

confidence: 99%

Antimicrobial Susceptibilities of Listeria monocytogenes Isolated from the Imported and the Domestic Foods in Japan

Okada¹

2015

JFNS

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“…Listeria isolates from food, environment, animal and human sources have been found to be resistant to commonly used antibiotics, including gentamicin, cotrimixazole and ofloxacin (Low & Donachie 1997;George 2002;Rahimi et al 2012;Soni et al 2013;Ndahi et al 2014). Since chemotherapy with antibiotics is the only available method for treatment of listeriosis, judicious use of these drugs in medical and veterinary practices along with monitoring of antibiotic susceptibility is very important (Okada et al 2011;Barbosa et al 2013).…”

Section: Veterinary Quarterly 11mentioning

confidence: 99%

“…Most of the time, infection in animals is subclinical but severe forms can also occur (OIE 2014). The disease is characterized by septicaemia, encephalitis, meningitis, meningoencephalitis, rhombencephalitis, abortion, stillbirth, perinatal infections and gastroenteritis (Brugere-Picoux 2008; Barbuddhe & Chakraborty 2009;Okada et al 2011; Barbuddhe et al 2012;Disson & Lecuit 2013;Limmahakhun & Chayakulkeeree 2013;Mateus et al 2013;OIE 2014). The organism has an intracellular life cycle that can pass from cell to cell without release from the cell.…”

Section: Introductionmentioning

confidence: 99%

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Dhama

Karthik

Tiwari

et al. 2015

Veterinary Quarterly

127

108

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Listeriosis is an infectious and fatal disease of animals, birds, fish, crustaceans and humans. It is an important food-borne zoonosis caused by Listeria monocytogenes, an intracellular pathogen with unique potential to spread from cell to cell, thereby crossing blood-brain, intestinal and placental barriers. The organism possesses a pile of virulence factors that help to infect the host and evade from host immune machinery. Though disease occurrence is sporadic throughout the world, it can result in severe damage during an outbreak. Listeriosis is characterized by septicaemia, encephalitis, meningitis, meningoencephalitis, abortion, stillbirth, perinatal infections and gastroenteritis with the incubation period varying with the form of infection. L. monocytogenes has been isolated worldwide from humans, animals, poultry, environmental sources like soil, river, decaying plants, and food sources like milk, meat and their products, seafood and vegetables. Since appropriate vaccines are not available and infection is mainly transmitted through foods in humans and animals, hygienic practices can prevent its spread. The present review describes etiology, epidemiology, transmission, clinical signs, post-mortem lesions, pathogenesis, public health significance, and advances in diagnosis, vaccines and treatment of this disease. Special attention has been given to novel as well as prospective emerging therapies that include bacteriophage and cytokine therapy, avian egg yolk antibodies and herbal therapy. Various vaccines, including advances in recombinant and DNA vaccines and their modes of eliciting immune response, are also discussed. Due focus has also been given regarding appropriate prevention and control strategies to be adapted for better management of this zoonotic disease.

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“…Harakeh et al (14) reported a high percentage of resistance to penicillin, ampicillin, and chlor amphenicol among L. monocytogenes isolates from dairybased food products. Very few studies on antibiotic resistance of L. monocytogenes have been conducted in Japan (32). Antimicrobial agents are used for growth promotion to improve animal husbandry in Japan.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Characteristics of Listeria monocytogenes in Bovine Colostrum in Japan

Hasegawa

Iwabuchi

Yamamoto

et al. 2013

Journal of Food Protection

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This study was conducted to determine the prevalence and characteristics of Listeria monocytogenes in bovine colostrum in Japan. We collected bovine colostrum samples from 210 dams from 21 dairy farms in Hokkaido prefecture (Japan) between March and June 2009. L. monocytogenes was detected in samples from 6 (28.6%) of the 21 farms. Of the 210 samples, 16 (7.6%) were positive for L. monocytogenes. We recovered 80 L. monocytogenes isolates; 44 (55%) isolates were classified as serotype 1/2b and 36 (45%) were classified as serotype 4b. The isolates were susceptible to penicillin, ampicillin, amoxicillin, gentamicin, kanamycin, streptomycin, erythromycin, vancomycin, tetracycline, chloramphenicol, ciprofloxacin, and trimethoprim-sulfamethoxazole. Pulsed-field gel electrophoresis (PFGE) characterization of the 80 isolates revealed six PFGE types. Two PFGE types corresponded to human listeriosis cases. Most L. monocytogenes isolates possessed virulence-associated genes (actA, hly, iap, inlA, inlC, mpl, plcA, plcB, opuCA, prfA, and clpC). One PFGE type isolate possessed an epidemic clone II marker. Our findings suggest that isolates from bovine colostrum have the potential to cause human and animal listeriosis. This is the first study on the prevalence and characteristics of L. monocytogenes isolated from bovine colostrum obtained from dairy farms. Our results have important implications for improving public health and elucidating the epidemiology of L. monocytogenes in bovine colostrum.

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Antimicrobial Susceptibilities of Listeria monocytogenes Isolated in Japan

Cited by 14 publications

References 18 publications

Antimicrobial Susceptibilities of Listeria monocytogenes Isolated from the Imported and the Domestic Foods in Japan

Antimicrobial Susceptibilities of Listeria monocytogenes Isolated from the Imported and the Domestic Foods in Japan

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Prevalence and Characteristics of Listeria monocytogenes in Bovine Colostrum in Japan

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