Immunology and the Skin Current Concepts

Longley, B. Jack; Braverman, Irwin M.; Rl, Edelson

doi:10.1111/j.1749-6632.1988.tb18810.x

Cited by 12 publications

(2 citation statements)

References 42 publications

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“…이러한 ROS로 인한 산화 적 스트레스로 인해 야기되는 산화물질의 불균형 및 산화 억제 기 능에 대응하기 위하여 체내에는 이를 방어하는 SOD, CAT, GPX, glutathione reductase (GR), HO1 등의 효소가 존재한다 (Jakus, 2000 (Kensler et al, 2007). NRF2는 정상적인 조건에서는 Kelch-like ECH-associated protein 1 (KEAP1)에 결합 되어 세포질 내에 존재하나 ROS와 같은 자극을 받게 되면 KEAP1으 로부터 유리되어 핵 내로 이동하여 핵 내에 존재하는 Maf 단백질과 결합하여 dimer를 이루고 항산화 효소의 promoter 부위에 존재하는 ARE와 결합함으로써 GR, HO1, NAD(P)H quinone dehydrogenase 1 (NQO1), gamma-glutamate cysteine ligase (γGCL), glutathione S-transferase (GST)와 같은 항산화 효소들의 발현을 증가시킨다 (Kang et al, 2005;Lee & Surh, 2005;Lee et al, 2017 (Baeuerle, 1998;Longley et al, 1988;Takeda et al, 2003).…”

Section: Introductionunclassified

Anti-oxidant and Anti-inflammatory Effects of Sinapic Acid in UVB Irradiation-Damaged HaCaT Keratinocytes

Lim¹,

Ku²,

Rhie³

et al. 2017

Asian J Beauty Cosmetol

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Purpose: The purpose of the present study was to explore the possibility of using sinapic acid as a skin anti-aging cosmetic by demonstrating its antioxidant and anti-inflammatory effects in keratinocytes damaged by ultraviolet (UV) irradiation. Methods: The effects of sinapic acid against oxidative stress were examined in a UVB-induced aging model of human keratinocyte HaCaT cells. The water-soluble tetrazolium salt (WST-1) assay was employed to examine protective effects against UVB. Changes in the concentration of reactive oxygen species (ROS) produced by UVB were measured using 2',7'-dichlorofluorescin diacetate (DCF-DA). Expression of genes associated with anti-oxidant and anti-inflammatory effects were confirmed using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Sinapic acid protected cells from UVB-induced cytotoxicity and reduced ROS produced by UVB irradiation. Expression of genes associated with antioxidant effects including, superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 1 (GPX1), nuclear factor (erythroid-derived 2)-like 2 (NRF2), and heme oxygenase 1 (HO1), increased and lipid peroxidation decreased in a dose-dependent manner. The expression of cyclooxygenase 2 (COX2), interleukin 6 (IL6), and interleukin 8 (IL8) genes, which are associated with inflammation, also decreased in a dose-dependent manner. Conclusion: These results suggest that sinapic acid has potential as an anti-aging cosmetic ingredient with anti-oxidant, anti-inflammatory properties against UVB irradiation.

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Section: Introductionunclassified

Anti-oxidant and Anti-inflammatory Effects of Sinapic Acid in UVB Irradiation-Damaged HaCaT Keratinocytes

Lim¹,

Ku²,

Rhie³

et al. 2017

Asian J Beauty Cosmetol

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“…http://dx.doi.org/10.20402/ajbc.2017.0183 증 반응을 유도하는 유전자의 발현을 촉진시킨다 (Baeuerle, 1988;Longley et al, 1988 (Kang et al,2012;Lee et al, 2017;Lee et al, 2016).…”

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Inhibition of Apoptosis and Anti-inflammatory Effects of Embelin

Yong¹,

Kim²,

Ahn³

et al. 2017

Asian J Beauty Cosmetol

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Purpose: Embelin is a major active ingredient of Embelia ribes Burm, belonging to Myrsinaceae, and an important traditional medicinal plant of Indian origin. Embelin has been studied for anti-cancer, anti-inflammatory, and anti-oxidative effects. However, there have been no studies on the use of cosmetic raw materials for embelin. Therefore, in this study, we investigated the inhibition of apoptosis and the anti-inflammatory effect to confirm the possibility of embelin as a cosmetic material. Methods: Cell viability was measured by using water-soluble tetrazolium salt (WST-1) assay. Quantitative real time polymerase chain reaction (qRT-PCR) was performed to quantitatively analyze the gene expression patterns of embelin-induced inhibition of apoptosis and anti-inflammatory effects in human dermal fibroblasts (HDFs). Apoptosis enzyme linked immunosorbent assay (ELISA) kit was used to confirm the degree of apoptosis. 5,5', 6,6'-tetrachloro-1,1', 3,3'-tetraethylbenzimidazolylcarbo cyanine iodide (JC-1) mitochondrial membrane potential detection kit was used to measure mitochondrial membrane potential. Results: The WST-1 assay confirmed that the reduced cell viability by hydrogen peroxide (H 2 O 2) was restored by embelin in a concentration-dependent manner. H 2 O 2-induced apoptosis was restored by embelin, Bcl-2-associated X protein (BAX) gene expression was decreased and mitochondrial membrane potential difference was increased in a concentration dependent manner. It was confirmed that embelin inhibits apoptosis in HDFs treated with H 2 O 2. In order to investigate the anti-inflammatory effect of embelin in H 2 O 2-treated human dermal fibroblasts, gene expression of cyclooxygenase 2 (COX2), tumor necrosis factor α (TNFα), and interleukin 6 (IL6), which induce inflammation, was examined and found to be decreased by embelin in a concentration dependent manner. Conclusion: These results suggest that embelin has a potential as an anti-aging cosmetic ingredient with inhibition of apoptosis and anti-inflammatory properties.

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Uptake of antigen by afferent lymph dendritic cells mediated by antibody

1990

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Dendritic cells isolated from sheep afferent lymph were examined for their ability to bind soluble protein and peptide antigens labeled with fluorescein both in in vitro assays and following intradermal injection of antigen in vivo. Analysis of dendritic cells by flow cytometry revealed weak direct binding of proteins and peptide antigens. However, the degree of uptake was greatly enhanced in the presence of specific antibody in vitro or if antigen was injected intradermally into antigen-primed sheep. About 60% of dendritic cells possessed the ability to take up antigen in both the in vitro and in vivo experiments. The uptake of antigen occurred very rapidly, reaching maximum values in terms of cell numbers and fluorescence intensity in less than 5 min in vitro and 20-40 min following in vivo challenge. Both sheep IgG subclasses could mediate this effect, but F(ab')2 fragments were ineffective. Procedures adopted to remove complement components from the in vitro test mixtures did not result in any reduction in the binding of antigen by dendritic cells. Two-color flow cytometry analysis of the dendritic cell population further showed that 43% of cells taking up the antigen/antibody complexes were CD1+, suggesting a relationship between these cells and Langerhans' cells or other dendritic cells in skin. The results, thus, indicate that approximately two thirds of sheep afferent lymph dendritic cells bind antigen/antibody complexes via an Fc receptor, a mechanism which could be important in the accentuated accessory function of these cells known to occur following secondary antigen challenge.

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Immunology and the Skin Current Concepts

Cited by 12 publications

References 42 publications

Anti-oxidant and Anti-inflammatory Effects of Sinapic Acid in UVB Irradiation-Damaged HaCaT Keratinocytes

Anti-oxidant and Anti-inflammatory Effects of Sinapic Acid in UVB Irradiation-Damaged HaCaT Keratinocytes

Inhibition of Apoptosis and Anti-inflammatory Effects of Embelin

Uptake of antigen by afferent lymph dendritic cells mediated by antibody

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