Immunological Studies with Synthetic Polypeptides

Sela, Michael

doi:10.1016/s0065-2776(08)60272-2

Cited by 203 publications

(62 citation statements)

References 190 publications

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“…In this manner it has been possible to identify relatively small units of several proteins that possess intact antigenic determinants (e.g., 1-5). A second approach has utilized synthetic polypeptide antigens as models for broadening our understanding of various molecular parameters (such as composition, size, shape, accessibility, electrical charge, optical configuration, and steric conformation) that control antigenicity (6,7).…”

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confidence: 99%

Antibodies Reactive with Native Lysozyme Elicited by a Completely Synthetic Antigen

Arnon

Maron

Sela

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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148

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A synthetic peptide consisting of the aminoacid sequence of residues 64-82 of lysozyme, with alanine replacing cysteine as residue 76, was prepared by the solid-phase technique. Mild reduction followed by reoxidation in air of the deprotected peptide led to the formation of a closed loop containing an intrachain disulfide bond. A conjugate consisting of this "loop" attached to multi-poly(DL-alanyl)-poly(L-lysine) elicited, in rabbits and goats, the formation of antibodies capable of reacting with lysozyme and with the loop peptide prepared from it. These immunological interactions can be inhibited by either lysozyme or the loop peptide, but not by the performic acid-oxidized open-chain peptide. Thus, the antibodies elicited by the completely synthetic antigen show specificity toward the "loop" structure (residues 64-80) of native lysozyme.

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confidence: 99%

Antibodies Reactive with Native Lysozyme Elicited by a Completely Synthetic Antigen

Arnon

Maron

Sela

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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148

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“…In these respects peptides and proteins of known amino acid sequence and/or stucture offer excellent immunochemical tools. Studies utilizing these systems have been recently reviewed (1)(2)(3)(4).…”

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confidence: 99%

“…Binding between the C-terminal pentapeptide and pooled globulins obtained from TMVP-sensitized guinea pigs was also shown. 1 The work on the immunochemistry of the TMVP peptides was hitherto aimed primarily at elucidating the principles underlying the binding of the determinant with antibodies to the protein. In view of the findings described, it was apparent that the use of this well-characterized system would serve as a powerful tool for the investigation of cellular immune responses as well.…”

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confidence: 99%

Studies on the Immune Response to a Characterized Antigenic Determinant of the Tobacco Mosaic Virus Protein

S̄pitler

Benjamini

Young

et al. 1970

The Journal of Experimental Medicine

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The following peptides have previously been shown to bind specifically with antibodies to TMVP: (a) An eicosapeptide representing residues 93–112 of TMVP and having the sequence Ileu-Ileu-Glu-Val-Glu-AspNH2-GluNH2-Ala-AspNH2-Pro-Thr-Thr-Ala-Glu-Thr-Leu-Asp-Ala-Thr-Arg. (b) Its C-terminal decapeptide. (c) Its C-terminal pentapeptide. (d) N-octanoyl-C-terminal-tripeptide. (e) (Lys)4-C-terminal-pentapeptide. (f) (Lys)7 C-terminal-pentapeptide. The present communication deals with the investigation of several parameters of the immunological activity of the peptides. The results show that none of the peptides tested were immunogenic in guinea pigs, nor did they stimulate the incorporation of 14C-thymidine by spleen cells derived from TMVP-primed animals. Results also showed that all of the peptides tested could elicit specific delayed and immediate skin reactions in TMVP-sensitized guinea pigs, and furthermore, that the peptides could specifically inhibit the migration of peritoneal exudate cells derived from these animals. The elicitation of delayed skin reactions and the ability to inhibit migration of peritoneal exudate cells were independent of carrier specificity.

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“…We, therefore, replaced the gelatin with multichain poly-DL-alanine as the carrier for peptides of tyrosine and glutamic acid and showed that the resulting copolymer, denoted (T,G)-A-L, led to specific, precipitable antibodies in experimental animals. At that stage Sara Fuchs joined us, and we synthesized numerous linear and multichain polyamino acids and tested them for immunogenicity and for antigenic specificity (Sela, 1966(Sela, , 1969. The availability of synthetic antigens permitted a systematic elucidation of the molecular basis of antigenicity.…”

Section: Back To Immunology: Synthetic Antigensmentioning

confidence: 99%