Immunological Studies in Amebiasis: Isotypic Characterization of Specific Antibodies by Enzyme-Linked Immunofiltration Assay

Pinon, J. M.; Poirriez, J.; Rémy, G.; Lepan, H.

doi:10.4269/ajtmh.1987.37.290

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Regression of the serum anti-amebic IgM antibody response occurs within three months in 50% of cases and reappearance or a later increase in anti-amebic IgM antibody titer represents an important prognostic factor. 3 The relatively short duration of the anti-amebic IgM antibody response has also been confirmed by Jackson and others 4 compared with the persistence of anti-amebic IgG serum antibodies for years after cure of invasive amebiasis. 5 Serum IgM antibodies to amebic plasma membrane antigens were detected previously with high specificity and sensitivity by an ELISA procedure.…”

mentioning

confidence: 73%

Serum IgM antibody response to the galactose-inhibitable adherence lectin of Entameoba histolytica.

Abd-Alla¹,

Jackson²,

Ravdin³

1998

The American Journal of Tropical Medicine and Hygiene

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Abstract. An ELISA for detection of serum IgM antibodies to the galactose-inhibitable adherence lectin of Entamoeba histolytica revealed that 2.8% of uninfected controls, 0.0% of controls infected with other parasites, 13.4% of asymptomatic amebic infections, 55% of colitis patients, and 77% of amebic liver abscess patients from Cairo, Egypt and Durban, South Africa had serum anti-lectin IgM antibodies. Of acute amebic colitis patients with symptoms for less than one week, only 6% possessed serum IgG anti-lectin antibodies, yet 45% had serum IgM antibodies to the amebic lectin. This compares with 65% of sera in acute colitis patients positive for lectin antigen as determined by ELISA with anti-lectin monoclonal antibodies. In conclusion, an ELISA for serum anti-lectin IgM antibodies appears to have greater clinical utility in the setting of acute amebic colitis than an ELISA for anti-lectin IgG antibodies, but is no more sensitive than an ELISA for detection of lectin antigen in sera.In some protozoal infections, the measurement of IgM antibodies is helpful in identification of current and postinfection states, such as giardiasis 1 and toxoplasmosis. 2 Detection of anti-Entamoeba histolytica IgM antibodies may constitute an important strategy for early diagnosis. Regression of the serum anti-amebic IgM antibody response occurs within three months in 50% of cases and reappearance or a later increase in anti-amebic IgM antibody titer represents an important prognostic factor. 3 The relatively short duration of the anti-amebic IgM antibody response has also been confirmed by Jackson and others 4 compared with the persistence of anti-amebic IgG serum antibodies for years after cure of invasive amebiasis. 5 Serum IgM antibodies to amebic plasma membrane antigens were detected previously with high specificity and sensitivity by an ELISA procedure. 6 Reactivity of anti-amebic IgM antibodies to small (5-15 kD) cytoplasmic membrane antigens has been demonstrated. 6 The use of well-characterized native amebic surface antigens may improve the diagnostic utility of serologic tests. The galactose-inhibitable lectin of E. histolytica mediates adherence of trophozoites to colonic mucins, epithelial cells, and host inflammatory cells. 7-9 Galactose-inhibitable lectin binding is absolutely required for amebic lysis of mammalian cells. 8 We used an ELISA with purified native amebic lectin, 10,11 which contains a highly conserved 170-kD antigen subunit that is recognized by serum IgG antibodies from patients worldwide with invasive amebiasis. 10,12-14 Lectin antigenemia has been demonstrated in patients with invasive amebiasis. 15,16 Amebic lectin has been detected in feces from patients with E. histolytica or E. dispar intestinal infections. 15,17 Previous studies have demonstrated that subjects with other parasitic infections do not generate antibodies to the amebic lectin and that anti-lectin monoclonal antibodies are specific for E. histolytica and E. dispar lectin epitopes. 11,13,15,16 In our study, we determined whether dete...

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mentioning

confidence: 73%

Serum IgM antibody response to the galactose-inhibitable adherence lectin of Entameoba histolytica.

Abd-Alla¹,

Jackson²,

Ravdin³

1998

The American Journal of Tropical Medicine and Hygiene

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“…The protein content of the supernatant, corresponding to the soluble antigenic extract, was determined by the method of Lowry et al (14). The antigen was stored frozen in aliquots of 0.1 ml at -80°C and used at a protein concentration of 4 mg/ml for CIE on cellulose acetate membrane (Sartorius, Paris, France) as described by Pinon et al (17). In our laboratory, the test was considered positive on the appearance of at least two precipitating bands.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a new bicolored latex agglutination test for immunological diagnosis of hepatic amoebiasis

Robert¹,

Mahaza²,

Bernard³

et al. 1990

J Clin Microbiol

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A new bicolored latex agglutination amoeba test (BLA) for detection of antibodies against Entamoeba histolytica was evaluated for its practicability and diagnostic sensitivity and specificity. BLA is rapid (5 min) and simple to perform. It requires only 20 ,l of a 1/3-diluted serum, 17 ,lI of reagent, and a glass slide. Reading of the test is easy because a positive result shows a green spot with a red surrounding edge. This bicolored pattern is easily distinguishable from the negative test result, which shows a homogeneous dark-brown spot. By using serum samples from 348 individuals, BLA was compared with immunofluorescence assay, indirect hemagglutination, and counterimmunoelectrophoresis. Sensitivity, specificity, efficiency, and positive and negative predictive values of the four methods were almost identical. The results of this study indicate that BLA could be very useful both as a screening method for the diagnosis of invasive amoebiasis and for epidemiological purposes.

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Immunological Studies in Amebiasis: Isotypic Characterization of Specific Antibodies by Enzyme-Linked Immunofiltration Assay

Cited by 2 publications

References 13 publications

Serum IgM antibody response to the galactose-inhibitable adherence lectin of Entameoba histolytica.

Serum IgM antibody response to the galactose-inhibitable adherence lectin of Entameoba histolytica.

Evaluation of a new bicolored latex agglutination test for immunological diagnosis of hepatic amoebiasis

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