Serum IgM antibody response to the galactose-inhibitable adherence lectin of Entameoba histolytica.

Abd-Alla, Mohamed D.; Jackson, Terry G. F. H.; Ravdin, Jonathan I.

doi:10.4269/ajtmh.1998.59.431

Cited by 32 publications

(26 citation statements)

References 24 publications

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“…Conversely, because the serosurvey detected a sustained antibody response that persisted even after the infection, 33,34 it contributed to the higher prevalence of infection in the present study compared with that in the 1992 survey. The seroprevalence we found in the current study provides an overview of E. histolytica infection in China, but does not provide information on the incidence of amebiasis.…”

Section: Discussioncontrasting

confidence: 40%

Seroprevalence of Entamoeba histolytica Infection in China

Yang¹,

Chen²,

Wu³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. The seroprevalence of Entamoeba histolytica infection in the residents of seven provinces in China was examined by using an enzyme-linked immunosorbent assay with a crude antigen and a recombinant surface antigen, C-Igl, of the parasites. A total of 1,312 serum samples were investigated. The positivity rates for these two antigens were 11.05% and 6.25%, respectively. There was no significant difference in the seropositivity to E. histolytica between men and women. We used a logistic regression model and maximal-likelihood methods to estimate the prevalence of E. histolytica infection from sequential serologic data. Seropositivity in Sichuan, Guizhou, and Sinkiang Provinces was higher than that in Beijing, Shanghai, and Qinghai Provinces. The present study provides an overview of seropositivity to E. histolytica infection in seven provinces in China and use the logistic regression model estimation method to achieve a more accurate measure of amebiasis prevalence.

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Section: Discussioncontrasting

confidence: 40%

Seroprevalence of Entamoeba histolytica Infection in China

Yang¹,

Chen²,

Wu³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…The purified lectin in native and recombinant forms is a highly conserved antigen. In over 95% of samples obtained from hundreds of patients cured of amebic colitis or liver abscess studied worldwide, native lectin protein purified from a single cloned E. histolytica isolate is recognized by serum immunoglobulin G (IgG), IgM, and IgA antibodies (1,3,5,6,21,32). The same has been found from subjects with noninvasive asymptomatic E. histolytica intestinal infection (28,31).…”

mentioning

confidence: 62%

Intestinal Antilectin Immunoglobulin A AntibodyResponse and Immunity to Entamoeba dispar Infection followingCure of Amebic LiverAbscess

Ravdin¹,

Abd-Alla²,

Welles

et al. 2003

Infect Immun

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We followed 93 subjects with amebic liver abscess (ALA) and 963 close associate controls at 3-month intervals for 36 months to characterize intestinal and humoral antibody responses to the amebic galactoseinhibitable lectin and to determine whether immunity developed to Entamoeba histolytica or Entamoeba dispar infection following cure of ALA. We found that ALA subjects had a higher prevalence and level of intestinal antilectin immunoglobulin A (IgA) and serum anti-LC3 (cysteine-rich recombinant lectin protein) IgA and IgG antibodies, P < 0.01 and P < 0.05, respectively, compared to controls. The intestinal antilectin IgA antibody response was sustained over a longer time period in ALA subjects (71.8% remained positive at 18 months and 52.6% at 36 months, P < 0.001 compared to 17.6% and 10.3% of controls, respectively). ALA subjects were highly immune to E. dispar infection throughout the study (0% infected at 6 and 36 months, compared to 6.5% and 4.9% of control subjects, respectively, P < 0.05). Upon entry into the study, 6.3% of ALA subjects were infected with E. histolytica; the incidence of new E. histolytica infections in controls (as determined by culture) was too low (1.4%) to determine whether ALA subjects exhibited immunity to new infections. We found that stool cultures every 3 months markedly underestimated the occurrence of new E. histolytica infections, as 15.3% of controls seroconverted after 12 months of follow-up. Unfortunately, under the field conditions present in Durban, South Africa, enzyme-linked immunosorbent assay for detection of lectin antigen in stool yielded unreliable results. In summary, subjects cured of ALA exhibited sustained mucosal IgA antibody responses to the amebic galactose-inhibitable lectin and a high level of immunity to E. dispar infection. Determination of immunity to E. histolytica following cure of ALA will require the use of more sensitive and reliable diagnostic methods.One of the major questions in amebiasis research is whether cure of invasive disease is followed by development of immunity to new intestinal infections and, thus, recurrence of disease. The enteric protozoan Entamoeba histolytica is one of the leading parasitic causes of death worldwide. Disease results from the parasite's ability to invade the colon, causing amebic colitis, or spreading via the portal venous system to the liver, resulting in formation of an amebic liver abscess (ALA). Amebic liver abscesses are more common in adult men and were thought to be fatal if untreated (7). A recent study in Hue, Vietnam, revealed that ALA is even more common than previously realized and may occur frequently in a subclinical manner (10). One large noncontrolled study reported that the rate of recurrence of amebic liver abscesses over 5 years in a highrisk population was less than expected compared to historical controls (14). In a cross-sectional study, the point prevalence of Entamoeba species intestinal infection was lower in subjects who possessed serum antiamebic antibodies (13).The E. histolytica gala...

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“…Serum IgG antibodies persist for years after E. histolytica infection, whereas the presence of IgM antibodies is shortlived and can be detected during the present or current infection. An ELISA for detection of serum IgM antibodies to amebic adherence lectin was successfully used with patients suffering from acute colitis for less than 1 week, as 45% had detectable antilectin IgM antibodies (1). In another study, it was shown that an assay based on the detection of anti-LC3 (recombinant cysteine-rich portion of the E. histolytica galactose-inhibitable lectin's 170-kDa subunit) antibodies in saliva is a more sensitive and specific test for diagnosis of ALA and acute amebic colitis than detection of serum anti-LC3 IgG antibodies (2).…”

Section: Antibody Detection Testsmentioning

confidence: 99%

Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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SUMMARY The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. Although recent studies highlight the recovery of E. dispar and E. moshkovskii from patients with gastrointestinal symptoms, there is still no convincing evidence of a causal link between the presence of these two species and the symptoms of the host. New approaches to the identification of E. histolytica are based on detection of E. histolytica-specific antigen and DNA in stool and other clinical samples. Several molecular diagnostic tests, including conventional and real-time PCR, have been developed for the detection and differentiation of E. histolytica, E. dispar, and E. moshkovskii in clinical samples. The purpose of this review is to discuss different methods that exist for the identification of E. histolytica, E. dispar, and E. moshkovskii which are available to the clinical diagnostic laboratory. To address the need for a specific diagnostic test for amebiasis, a substantial amount of work has been carried out over the last decade in different parts of the world. The molecular diagnostic tests are increasingly being used for both clinical and research purposes. In order to minimize undue treatment of individuals infected with other species of Entamoeba such as E. dispar and E. moshkovskii, efforts have been made for specific diagnosis of E. histolytica infection and not to treat based simply on the microscopic examination of Entamoeba species in the stool. The incorporation of many new technologies into the diagnostic laboratory will lead to a better understanding of the public health problem and measures to control the disease.

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Serum IgM antibody response to the galactose-inhibitable adherence lectin of Entameoba histolytica.

Cited by 32 publications

References 24 publications

Seroprevalence of Entamoeba histolytica Infection in China

Seroprevalence of Entamoeba histolytica Infection in China

Intestinal Antilectin Immunoglobulin A AntibodyResponse and Immunity to Entamoeba dispar Infection followingCure of Amebic LiverAbscess

Laboratory Diagnostic Techniques for Entamoeba Species

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