Immunological responses of the mangrove oysters Crassostrea gasar naturally infected by Perkinsus sp. in the Mamanguape Estuary, Paraíba state (Northeastern, Brazil)

Queiroga, Fernando Ramos; Marques‐Santos, Luis Fernando; Hégaret, Hélène; Soudant, Philippe; Farias, Natanael Dantas; Schlindwein, Aline Daiane; Silva, Patrícia Mirella da

doi:10.1016/j.fsi.2013.04.034

Cited by 25 publications

(21 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the high prevalence in this month reflects the cumulative effect of the dry season, when the highest water temperatures and salinities are recorded. It was in May that these oysters started to suffer an impairment of the immune system (Queiroga et al 2013). Perkinsus marinus and P. olseni are strongly associated with high temperatures and salinities (see reviews of Villalba et al 2004Villalba et al , 2011Choi and Park, 2010).…”

Section: Histopathological Analysismentioning

confidence: 99%

Parasites infecting the cultured oyster Crassostrea gasar (Adanson, 1757) in Northeast Brazil

et al. 2015

Self Cite

View full text Add to dashboard Cite

The oyster Crassostrea gasar is a species widely used as food and a source of income for the local population of the estuaries of Northeast Brazil. Perkinsus marinus and Perkinsus olseni are deleterious parasites for oyster farming and were recently detected in Brazil. In this study, a histopathologic survey of the oyster C. gasar cultured in the estuary of the River Mamanguape (Paraíba State) was performed. Adult oysters were collected in December 2011 and March, May, August and October 2012 and processed for histology and Perkinsus sp. identification by molecular analyses. Histopathological analysis revealed the presence of parasitic organisms including viral gametocytic hypertrophy, prokaryote-like colonies, protozoans (Perkinsus sp. and Nematopsis sp.) and metazoans (Tylocephalum sp. and cestodes). Other commensal organisms were also detected (the protozoan Ancistrocoma sp. and the turbellarian Urastoma sp.). The protozoan parasite Perkinsus sp. had the highest overall prevalence among the symbiotic organisms studied (48.9%), followed by Nematopsis sp. (36.3%). The other organisms were only sporadically observed. Only the protozoan Perkinsus sp. caused alterations in the oysters' infected organs. Molecular analyses confirmed the presence of P. marinus, P. olseni and Perkinsus beihaiensis infecting the oyster C. gasar. This is the first report of P. beihaiensis in this oyster species.

show abstract

Section: Histopathological Analysismentioning

confidence: 99%

Parasites infecting the cultured oyster Crassostrea gasar (Adanson, 1757) in Northeast Brazil

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Perkinsus spp. were recently reported in Brazil, infecting C. rhizophorae and C. gasar oysters and Anomalocardia brasiliana clams from the northeastern region (DA SILVA et al, 2013;FERREIRA et al, 2015;LUZ & BOEHS, 2016;QUEIROGA et al, 2013QUEIROGA et al, , 2015SABRY et al, 2009SABRY et al, , 2013. P. marinus and P. olseni are both notifiable to the World Organisation for Animal Health (OIE) and were reported to infect C. gasar oysters from the estuary of the São Francisco River, Sergipe State (DA SILVA et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Growth, mortality and susceptibility of oyster Crassostrea spp. to Perkinsus spp. infection during on growing in northeast Brazil

Scardua

Vianna

Duarte

et al. 2017

Rev. Bras. Parasitol. Vet.

Self Cite

View full text Add to dashboard Cite

Crassostrea rhizophorae and C. gasar oysters are cultivated in the northeast region. Perkinsus parasites infect bivalves, and their effects on oysters from tropical regions are poorly understood. This study evaluated the impact of Perkinsus infection on the productive traits of native oysters. Oysters were sampled bimonthly during 7 months, from July 2010 to February 2011, to evaluate growth rate, mortality and shell color patterns (white and dark-gray) (n = 500), and to determine the prevalence and intensity of Perkinsus (n = 152). Perkinsus and Crassostrea species were determined using molecular tools. Results showed that most dark-gray (90%, n = 20) and white (67%, n = 18) oysters were C. gasar and C. rhizophorae, respectively. Oysters showed a high growth rate and moderate cumulative mortality (44%). C. gasar oysters grew better and showed lower mortality and lower incidence of Perkinsus compared to C. rhizophorae. The mean prevalence of Perkinsus was moderate (48%), but the infection intensity was light (2.2). Perkinsosis affected very small oysters (19.4 mm). In conclusion, native oysters, especially C. gasar, have a great potential for culture, mortality is not associated with perkinsosis, and the shell color of oysters can be used to improve selection for spats with better performance.

show abstract

“…with RFTM method observed in the present study for native oyster ( C. gasar ) was moderate to heavy. In the north‐eastern of Brazil, heavy prevalence, as detected by the RFTM method, was observed in C. gasar from Paraíba by Queiroga et al (), Queiroga et al () and from Sergipe State (da Silva et al, ). Additionally, in the case of C. gasar from Balneário Barra do Sul, where P. beihaiensis was the identified species by sequencing analysis (two cases), only seven cases of Perkinsus detected by histology, while 79 cases were detected by RFTM, which suggests that a low percentage of infections give rise to clinical signs.…”

Section: Discussionmentioning

confidence: 90%

“…Crassostrea gasar , another important native oyster species in Brazil, was reported to be infected by Perkinsus sp. in Paraíba (Queiroga et al, ), in Sergipe (da Silva, Scardua, Vieira, Alves, & Dungan, ) and in Rio Grande do Norte (da Silva, Costa, Araújo, Queiroga, & Wainberg, ) states; by P. beihaiensis in Paraíba (Queiroga, Vianna, Vieira, Farias, & Silva, ) and in Bahía (Luz et al, ) states; by P. marinus in Sergipe (da Silva et al, ) and in Paraíba (Queiroga et al, ) states; and by P. olseni in Sergipe (da Silva et al, ) and in Paraíba (Queiroga et al, ) states. Interestingly, da Silva et al () observed for the first time the presence of P. olseni and P. marinus infecting the same individual of C. gasar .…”

Section: Introductionmentioning

confidence: 99%

Two epizootic Perkinsus spp. events in commercial oyster farms at Santa Catarina, Brazil

Cunha

Pontinha

Castro

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

Perkinsus spp. have been detected in various bivalve species from north‐east Brazil. Santa Catarina is a South Brasil state with the highest national oyster production. Considering the pathogenicity of some Perkinsus spp., a study was carried out to survey perkinsosis in two oyster species cultured in this State, the mangrove oyster Crassostrea gasar and the Pacific oyster Crassostrea gigas. Sampling involved eight sites along the state coast, and oyster sampling was collected during the period between January 2013 and December 2014. For the detection of Perkinsus, Ray's fluid thioglycollate medium (RFTM) and histology were used, and for the identification of the species, PCR and DNA sequencing were used. Perkinsus spp. was found by RFTM in C. gigas and C. gasar from São Francisco do Sul. This pathology was also detected in C. gasar from Balneário Barra do Sul both, by RFTM and histology. Perkinsus marinus was identified in C. gigas and C. gasar from São Francisco do Sul and Perkinsus beihaiensis in C. gasar from Balneário Barra do Sul. This is the first report of P. marinus in C. gigas from South America. Results of this preliminary study suggest that both oyster species tolerate the species of Perkinsus identified, without suffering heavy lesions.

show abstract

Immunological responses of the mangrove oysters Crassostrea gasar naturally infected by Perkinsus sp. in the Mamanguape Estuary, Paraíba state (Northeastern, Brazil)

Cited by 25 publications

References 74 publications

Parasites infecting the cultured oyster Crassostrea gasar (Adanson, 1757) in Northeast Brazil

Parasites infecting the cultured oyster Crassostrea gasar (Adanson, 1757) in Northeast Brazil

Growth, mortality and susceptibility of oyster Crassostrea spp. to Perkinsus spp. infection during on growing in northeast Brazil

Two epizootic Perkinsus spp. events in commercial oyster farms at Santa Catarina, Brazil

Contact Info

Product

Resources

About