Immunological responses in mice to full-thickness corneal grafts engineered from porcine collagen

Liu, Lei; Kuffová, Lucia; Griffith, May; Dang, Zexu; Muckersie, Elizabeth; Liu, Yuwen; McLaughlin, Christopher; Forrester, John V.

doi:10.1016/j.biomaterials.2007.04.025

Cited by 35 publications

(19 citation statements)

References 28 publications

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“…A recent study showed that full-thickness corneal grafts engineered from porcine collagen induced innate immunological responses with severe retrocorneal membrane formation in mice. 24 In contrast, we did not see any inflammatory or immune responses in the present study. Hypertonic saline might decrease the antigenicity of the collagen of the porcine cornea in our study, or it is possible that hypertonic saline exerts antiinflammatory=immunomodulatory properties as reported in previous studies.…”

Section: Figcontrasting

confidence: 64%

Processing Porcine Cornea for Biomedical Applications

Kim

Lee

et al. 2009

Tissue Engineering Part C: Methods

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To investigate the propriety of decellularized porcine corneas as a source of lamellar corneal xenografts, we treated porcine corneas with (1) freezing, (2) three freezing-thawing, (3) hypertonic saline, (4) hyperosmolar glycerol, (5) trypsin/sodium dodecyl sulfate/Dispase, and (6) DNase/RNase. After processing, we examined the cells and collagen structures of the decellularized corneas using hematoxylin-eosin staining, terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay, and transmission electron microscopy. Cell viability was also assessed via organ culture. In addition, the outcomes of porcine anterior lamellar corneal xenografting were evaluated in rabbits. Graft integration and corneal thickness were assessed using anterior optical coherence tomography, and the corneas were histologically examined sequentially after transplantation. We found that porcine corneas treated with hypertonic saline-based decellularization had little immunogenicity with intact collagen structures. The porcine corneal xenografts decellularized with the hypertonic saline-based method were well integrated into the adjacent host tissues and remained clear in rabbit eyes for more than 6 months.

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Section: Figcontrasting

confidence: 64%

Processing Porcine Cornea for Biomedical Applications

Kim

Lee

et al. 2009

Tissue Engineering Part C: Methods

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“…Preliminary studies have shown that, initially, a low-grade, self-limiting inflammatory response occurs when these EDC/NHS cross-linked gels are implanted through fullthickness penetrating into mice. 25 The main obstacle encountered in these full-thickness grafts was the formation of a retro corneal membrane. However, because the mouse corneas were small, the implants overlapped the adjacent, surrounding host tissue, making it possible for host stromal cells to grow onto the overlying implants and thus give rise to a retroprosthetic membrane.…”

Section: Discussionmentioning

confidence: 99%

Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

McLaughlin¹,

Muzakare²,

Lagali³

et al. 2008

Cornea

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“…In a pig study, innervation was present by 3 mo, and a subepithelial nerve plexus was visible by 6 mo; an additional in vivo confocal microscopy study using these constructs defined a pattern of innervation beginning by 2 months in the deep anterior stroma and progressing to the recovery of preoperative nerve density in the subbasal layer by 1 year after transplantation (58). This construct's ability to serve as a full thickness corneal graft was evaluated in a murine model with full penetrating keratoplasty (59). Although the grafts were mechanically stable, the recipient eyes remained transparent for only 10 days, and then became progressively opaque; a significant inflammatory response was observed, resulting in the formation of a retro-corneal fibrin membrane below the graft with infiltrating myeloid cells.…”

Section: Advances In Tissue-engineered Corneal Equivalentsmentioning

confidence: 98%

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

et al. 2008

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ABSTRACT:The field of corneal tissue engineering has made many strides in recent years. The challenges of engineering a biocompatible, mechanically stable, and optically transparent tissue are significant. To overcome these challenges, researchers have adopted two basic approaches: cell-based strategies for manipulating cells to create their own extracellular matrix, and scaffold-based strategies for providing strong and transparent matrices upon which to grow cells. Both strategies have met with some degree of success. In addition, recent advances have been made in innervating a tissueengineered construct. Future work will need to focus on further improving mechanical stability of engineered constructs as well as improving the host response to implantation.

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Immunological responses in mice to full-thickness corneal grafts engineered from porcine collagen

Cited by 35 publications

References 28 publications

Processing Porcine Cornea for Biomedical Applications

Processing Porcine Cornea for Biomedical Applications

Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

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