Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

McLaughlin, Christopher; Muzakare, Lea; Lagali, Neil; Forrester, John V.; Kuffová, Lucia; Rafat, Mehrdad; Liu, Yuwen; Shinozaki, Naoshi; Vascotto, Sandy G.; Munger, Réjean; Griffith, May

doi:10.1097/ico.0b013e3181658408

Cited by 28 publications

(13 citation statements)

References 30 publications

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“…First, the ECM acts as a biological scaffold that provides the appropriate microenvironment for corneal repair and regeneration during wound healing. 16 Second, the ECM, composed mainly of collagen and representing >70% of the dry weight of the cornea, is essential for corneal transparency because of the regular arrangement of uniformly thin collagen fibrils into lamellae. 17,18 Therefore, much effort has been expanded to develop efficient ECM for tissue-engineered corneal equivalents, although none has been clinically usable.…”

Section: Discussionmentioning

confidence: 99%

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Processing Porcine Cornea for Biomedical Applications

Kim

Lee

et al. 2009

Tissue Engineering Part C: Methods

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To investigate the propriety of decellularized porcine corneas as a source of lamellar corneal xenografts, we treated porcine corneas with (1) freezing, (2) three freezing-thawing, (3) hypertonic saline, (4) hyperosmolar glycerol, (5) trypsin/sodium dodecyl sulfate/Dispase, and (6) DNase/RNase. After processing, we examined the cells and collagen structures of the decellularized corneas using hematoxylin-eosin staining, terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay, and transmission electron microscopy. Cell viability was also assessed via organ culture. In addition, the outcomes of porcine anterior lamellar corneal xenografting were evaluated in rabbits. Graft integration and corneal thickness were assessed using anterior optical coherence tomography, and the corneas were histologically examined sequentially after transplantation. We found that porcine corneas treated with hypertonic saline-based decellularization had little immunogenicity with intact collagen structures. The porcine corneal xenografts decellularized with the hypertonic saline-based method were well integrated into the adjacent host tissues and remained clear in rabbit eyes for more than 6 months.

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Section: Discussionmentioning

confidence: 99%

“…Recellularization of a decellularized graft is important because keratocytes play an active role in the maintenance and metabolism of the corneal stroma. 16,22,23 In addition, this decellularized porcine corneal stroma could be used as a matrix for culturing human corneal cells in vitro and making a tissue-engineered cornea.…”

Section: Figmentioning

confidence: 99%

Processing Porcine Cornea for Biomedical Applications

Kim

Lee

et al. 2009

Tissue Engineering Part C: Methods

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“…Researchers reported no difference between type I and III collagen‐based substitutes with both being nerve‐friendly. These substitutes, following further clinical testing, show promise in helping to alleviate the shortage of corneal material and as emergency patch grafts 53–55 …”

Section: Emerging Devicesmentioning

confidence: 99%

Keratoprostheses in clinical practice – a review

Gomaa

Comyn

Liu

2010

Clinical Exper Ophthalmology

114

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The search for a substitute for the natural cornea dates back more than 200 years. Although several devices have been developed and trialled, very few have had successful long-term results and continue in regular clinical use. Keratoprosthesis (KPro) surgery is complex and should be performed in centres with an experienced multidisciplinary team. Currently available KPro devices range from the totally synthetic, such as the Boston KPro, to the totally biological tissue-engineered artificial cornea. The osteo-odonto keratoprothesis combines a synthetic optic with a biological haptic. All keratoprostheses have significant limitations, although visual improvement is possible with each of the devices in clinical use today. This review discusses these devices with emphasis on their indications, surgical techniques and results, before briefly exploring emerging devices and innovative approaches for the future.

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“…Previous experiments using collagen only scaffolds for corneal applications have demonstrated that EDC:NHS collagen hydrogels have an excellent microstructure to foster cell growth . The addition of both CNTs and chitosan to the collagen matrix prior to cross‐linking was shown not to have a significant impact on the microstructure of the hydrogel (Figure b).…”

Section: Resultsmentioning

confidence: 90%

Rational Design of a Conductive Collagen Heart Patch

Sherrell

Cieślar-Pobuda

Ejneby

et al. 2017

Macromolecular Bioscience

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Cardiovascular diseases, including myocardial infarction, are the cause of significant morbidity and mortality globally. Tissue engineering is a key emerging treatment method for supporting and repairing the cardiac scar tissue caused by myocardial infarction. Creating cell supportive scaffolds that can be directly implanted on a myocardial infarct is an attractive solution. Hydrogels made of collagen are highly biocompatible materials that can be molded into a range of shapes suitable for cardiac patch applications. The addition of mechanically reinforcing materials, carbon nanotubes, at subtoxic levels allows for the collagen hydrogels to be strengthened, up to a toughness of 30 J m and a two to threefold improvement in Youngs' modulus, thus improving their viability as cardiac patch materials. The addition of carbon nanotubes is shown to be both nontoxic to stem cells, and when using single-walled carbon nanotubes, supportive of live, beating cardiac cells, providing a pathway for the further development of a cardiac patch.

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Regeneration of Corneal Cells and Nerves in an Implanted Collagen Corneal Substitute

Cited by 28 publications

References 30 publications

Processing Porcine Cornea for Biomedical Applications

Processing Porcine Cornea for Biomedical Applications

Keratoprostheses in clinical practice – a review

Rational Design of a Conductive Collagen Heart Patch

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