Immunological response against allogeneic chondrocytes transplanted into joint surface defects in rats

Hyc, Anna; Malejczyk, Jacek; Osiecka, Anna; Moskalewski, Stanisław

doi:10.1016/s0963-6897(96)00254-0

Cited by 9 publications

(17 citation statements)

References 20 publications

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“…In our experimental model, chondrocytes isolated from the articular-epiphyseal cartilage complex (Van Sickle and Kinkaid, 1983) of 3-5-day-old inbred Lewis (Lew, Rt-1 1 ) or Wistar Albino Glaxo (WAG, Rt-1 u ) rats were suspended in high molecular weight hyaluronic acid (Robinson et al, 1990) and introduced into a hole made with an electric bore in a subpatellar region of the femur condyle of mature animals (Hyc et al, 1997;Moskalewski et al, 1993). Both syngeneic and allogeneic chondrocytes produced cartilage.…”

Section: Studies On Intracartilaginous Chondrocyte Transplants In Inbmentioning

confidence: 99%

“…The terms "transplants into the cartilage" or "intracartilaginous transplants" are thus not precise since, in practice, such transplants reach into subchondral bone and medullary spaces and are in contact with bone marrow cells. It is probably not particularly important in case of autogeneic or syngeneic transplants of articular chondrocytes, but cartilage produced in articular cartilage defects by allogeneic chondrocytes is rejected by infiltrating cells accumulating on the subchondral side of the transplant (Hyc et al, 1997;Kawabe and Yoshinao, 1991).…”

Section: Introductionmentioning

confidence: 99%

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Immune response by host after allogeneic chondrocyte transplant to the cartilage

Moskalewski

Hyc

Osiecka-Iwan

2002

Microscopy Res & Technique

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Chondrocytes constitutively express class I and, in some species, class II major histocompatibility complex (MHC). It is also possible that they possess specific differentiation antigen(s). Furthermore, lymphocytic cells, corresponding to NK cells, display spontaneous cytotoxic activity against chondrocytes. Studies on articular cartilage repair by transplants of allogeneic chondrocytes were mainly done on non-inbred animals, such as rabbits and hens. Surprisingly, only in single instances these transplants were rejected. In inbred rats, allogeneic chondrocytes transplanted into full-thickness defects in articular cartilage immediately after isolation evoked systemic immunological reaction and produced cartilage was rejected. Combined immunosuppression with cyclosporin A and cladribine did not prevent rejection of such transplants. Mechanical separation of transplants from bone marrow prevented sensitization of recipients and rejection of the produced cartilage. Successful allogeneic chondrocyte transplants in rabbits and hens could be tentatively explained by a certain degree of inbreeding among experimental animals, by the use of chondrocytes cultivated before grafting in artificial scaffolds and thus protected by matrix produced in vitro, and also by creation of a temporary mechanical barrier between transplant and bone marrow by tissues damaged during preparation of the defect.

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Section: Studies On Intracartilaginous Chondrocyte Transplants In Inbmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immune response by host after allogeneic chondrocyte transplant to the cartilage

Moskalewski

Hyc

Osiecka-Iwan

2002

Microscopy Res & Technique

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“…It is, however, necessary to consider this study to be only preliminary in the development of this application for the clinical practise. Even though the results of chondrocyte transplantation in the physis seem promising with respect to the possible prevention of the bone bridge formation, it is necessary to study the survival of chondrocytes, immunological aspects (Hyc et al 1997) as well as the possibility of influencing the proliferation of chondrocytes pharmacologically (Wakita et al 1998). …”

Section: Discussionmentioning

confidence: 99%

Transplantation of the Autogenous Chondrocyte Graft to Physeal Defects: an Experimental Study in Pigs

Gál¹,

Nečas²,

Adler³

et al. 2002

Acta Vet. Brno

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The aim of the experiment was to evaluate the possibility of preventing bone bridge formation in damaged distal femoral physis using implantation of autogenous chondrocyte graft. Ten pigs were used in the study. In each pig, a sample of cartilage of 3 × 3 mm in diameter was taken from non-weight-bearing surface of the lateral femoral condyle and used to culture chondrocytes for the preparation of the autogenous chondrocyte graft.Fourteen days later, a canal of 4 mm diameter was drilled through the distal femoral physis in the area of the lateral condyle. This canal was then filled with the autogenous chondrocyte graft. The same defect, left unfilled, was created in the physis of the medial condyle. One animal succumbed during the transplantation of the chondrocyte graft due to complications of anaesthesia.Remaining 9 animals were euthanised and examined histologically with respect to the healing of the transplant in the area of the growth plate. In all 9 cases the graft was healed in the distal femoral physis. Bone bridges were formed in canals unfilled with the autogenous chondrocyte graft.We conclude that transplantation of the autogenous chondrocyte graft into iatrogenically damaged physis in pigs can prevent bone bridge formation and growth arrest. This finding may have clinical implications in potential transplantation of chondrocyte autografts in children. Growth plate, injury, bone bridge, pigGrowth plate fractures in children, in particular those ones of types III to V according to the classification by Salter-Harris (1963), belong to injuries of poor prognosis. Damage to the regular chondrogenesis in the physis can result in altered growth of the bone affected due to the formation of a bone bridge. In dependence on its location we recognise either a peripheral bone bridge resulting in angular deformities of the bone affected, a central bone bridge shortening the bone without its angular deformity or a combined kind of a bridge causing both angular deformities and shortening of the bone (Rockwood et al. 1984). Combined bone bridges are least favourable for the patient (Rockwood et al. 1984). Iatrogenic damage to the physis during surgical repositioning of a physeal fracture can lead to the formation of osteonecrotic bone bridges and then can form a bone bridge in the fissure after incomplete repositioning of fragments (von Laer 1984). Clinical consequences of physeal bone bridging are often very serious (Bak and Boeckstyns 1997) and their therapy includes complex corrective surgeries (Hove and Engesaeter 1997) or complicated resections of bone bridges (Langenskiold 1981).Trauma to the distal femoral physis is a typical example of injury to the growth plate in children. This injury, common in preadolescents and adolescents, is not so frequent but can

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“…Unfortunately, transplants of viable allogeneic cartilages or freshly isolated donor chondrocytes evoke a strong immune response in the recipients that practically eliminates their use in routine plastic surgery. 37,43,44 Because the factors regulating differentiation of mesenchymal precursor cells toward chondrogenesis are not well identified, differentiated autologous chondrocytes remain the only feasible starting material, despite the fact that the number of cells from biopsies of autologous elastic cartilage is usually insufficient.…”

Section: Discussionmentioning

confidence: 99%

Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and ?-elastin

Chalain

Phillips

Hinek

1999

J. Biomed. Mater. Res.

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Transplantation of isolated chondrocytes has long been acknowledged as a potential method for rebuilding small defects in damaged or deformed cartilages. Recent advances in tissue engineering permit us to focus on production of larger amounts of cartilaginous tissue, such as might be needed for reconstructive surgery of the entire auricle. In this report we describe modification of the basic techniques that lead to production of a large amount of elastic cartilage originated from porcine and human isolated chondrocytes. Small fragments of auricular cartilage were harvested from children undergoing ear reconstruction for microtia or extirpation of preauricular tags and from ears of juvenile pigs. Enzymatically isolated elastic chondrocytes were then agitated in suspension to form the chondronlike aggregates, which were further embedded in molded hydrogel constructs made of alginate and type I collagen augmented with kappa-elastin. The constructs were then implanted in nude mice and harvested 4 and 12 weeks after heterotransplantation. The resulting neocartilage closely resembled native auricular cartilage at the gross, microscopic, and ultrastructural levels. Immunohistochemistry and electron microscopy additionally confirmed that the newly produced cartilage contained the major components of the elastic cartilage-specific matrix, including collagen type II, proteoglycans, and well-assembled elastic fibers.

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Immunological response against allogeneic chondrocytes transplanted into joint surface defects in rats

Cited by 9 publications

References 20 publications

Immune response by host after allogeneic chondrocyte transplant to the cartilage

Immune response by host after allogeneic chondrocyte transplant to the cartilage

Transplantation of the Autogenous Chondrocyte Graft to Physeal Defects: an Experimental Study in Pigs

Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and ?-elastin

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