Immunological relationship of extra-cellular polysaccharide antigens produced by different mould species

Notermans, S.; Soentoro, P.S.S.

doi:10.1007/bf00393467

Cited by 50 publications

(19 citation statements)

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“…One of the main problems in investigating the role of surface proteins in differentiation programmes and intercellular communication is the almost complete lack of methods for their isolation and analysis. Among the few approaches for gaining information about the molecular architecture of hyphal surfaces are immunological studies on cell wall associated polysaccharides [25] and ultrastructural investigations by scanning electron microscopy [26]. In this report we propose a reliable, easy procedure, based on washing intact fungal mycelia with 1 M LiCl, allowing the isolation of surface proteins without any detectable contaminations with intracellular material (see section 3.1).…”

Section: Discussionmentioning

confidence: 99%

Isolation and electrophoretic analysis of surface proteins of the zygomycete Absidia glauca

1988

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Methods for isolation and elcctrophoretic analysis of surface proteins from the mucoraceous fungus Absidia glauca are described. By analysing the set of cell wall associated proteins which can bc extracted with I M LiCI, we could show marked differences between submersed and aerial mycelium. Furthermore, during differentiation of the aerial mycelium protein patterns change remarkably.We were able to demonstrate the existence of mating type specific proteins, the most prominent of which is a 15 kDa polypcptide expressed in submersed as well as in aerial hyphae of the (+) mating type.

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Section: Discussionmentioning

confidence: 99%

Isolation and electrophoretic analysis of surface proteins of the zygomycete Absidia glauca

1988

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“…Por meio dos métodos tradicionais de cultivo, fungos danificados ou inativados pelo calor dificilmente são detectados e a detecção de micélio por microscopia não é precisa, enquanto os métodos químicos são particularmente laboriosos (NOTERMANS et al, 1986). Os métodos microbiológicos podem demorar em torno de 6-7 dias e necessita de pessoal com experiência (BANKS;COX;NORTTHWAY, 1993).…”

Section: Métodos Para Detecção De Fungosunclassified

“…Enquanto os métodos convencionais detectam o número de propágulos viáveis, os imunoensaios podem revelar a quantidade de biomassa fúngica (NOTERMANS et al, 1986;DE RUITER et al, 1996).…”

Section: Métodos Para Detecção De Fungosunclassified

“…O anticorpo gênero-específico permite a detecção de Fusarium na presença de outros fungos de maneira rápida e simples (GAN et al, 1997). Este teste também pode ser útil para avaliação pré-colheita de espécies de Fusarium em amostras de trigo, podendo ser um sinal de advertência quanto à possível produção de deoxinivalenol em cereais e seus derivados (ABRAMSON et al, 1998 Esses ensaios mostraram-se muito úteis não só na detecção de fungos, mas também na quantificação dos mesmos em alimentos, pelo fato de a produção de exoantígenos estar relacionada à biomassa fúngica (NOTERMANS; HEUVELMAN, 1985;SOENTORO, 1986;BANKS;COX;NORTHWAY, 1993;DEWEY et al, 1993).…”

Section: Métodos Para Detecção De Fungosunclassified

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Imunoensaios: uma alternativa para a detecção de fungos toxigênicos em alimentos

et al. 2006

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ResumoO monitoramento constante da contaminação fúngica é imprescindível para assegurar a qualidade e segurança dos alimentos, reduzindo as perdas econômicas, assim como os riscos à saúde humana e animal. Os métodos tradicionais de identificação e detecção de fungos (cultivo em diversos meios, exame microscópico e análises bioquímicas) geralmente consomem muito tempo e exigem pessoal com experiência. Os imunoensaios, particularmente os ensaios imunoenzimáticos, constituem uma alternativa promissora aos métodos tradicionais devido à alta sensibilidade, especificidade, reprodutibilidade e potencial como método rápido de controle de qualidade. Dentre os ensaios imunoenzimáticos, aqueles baseados em exoantígenos são os mais empregados na resolução de problemas taxonômicos, detecção e identificação de fungos toxigênicos. Nesta revisão serão abordados conceitos básicos de imunoensaios, métodos de detecção de fungos, assim como diversos ensaios imunoenzimáticos para a detecção de fungos toxigênicos em alimentos. Palavras-chave: Imunoensaios, fungos toxigênicos, detecção de fungos, imunodetecção AbstractConstant monitoring of mould contamination is essential in order to assure the food quality and safety and reduce the economic losses, as well as to minimize the potential hazards to human and animal health. The traditional methods for mould identification and detection (culture in several media, microscopic examination and chemical analysis) are usually time-consuming and require trained staff. Immunoassays, particularly enzyme-linked immunosorbent assay (ELISA) could be a promising alternative to the traditional methods due to high sensitivity, specificity, reproducibility and potential for use in rapid quality control. Among ELISAs, those based on exoantigens are the most employed in the resolution of taxonomic problems, detection and identification of toxigenic fungi. This review discusses the basic principles of immunoassays, methods of mould detection and the several ELISAs developed for toxigenic fungi detection in food.

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“…The finding that fungal polysaccharides have immunological properties, and that some of them are almost genus-specific (Notermans & Soentoro, 1986), enhanced interest in the elucidation of the structure of these molecules since they might be used as chemotaxonomic characters. Most studies on serological properties of fungal polysaccharides deal with exopolysaccharides (Notermans & Soentoro, 1986 ;Notermans et al, 1987 ;De Ruiter et al, 1992) and cell wall glycoproteins (Gailliez & Poulain, 1988 ;Sridhara et al, 1990 ;Hearn, 1991).…”

Section: Introductionmentioning

confidence: 99%

Galactomannans from the cell walls of species of Paecilomyces sect. Paecilomyces and their teleomorphs as immunotaxonomic markers

et al. 1999

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An alkali-extractable and water-soluble fraction (F1S) was obtained from cell walls of Paecilomyces variotii and species of the related genera Talaromyces, Byssochlamys and Thermoascus. The structure of the main polysaccharide of these fractions was studied and found to consist of a core of (1 6)-α-mannopyranose partially substituted at O-2 by chains of galactofuranose and shorter chains of mannopyranose. The differences in the regularity of the branching points and the length of the galactofuranose side chains are useful to distinguish between species. These differences were detected by immunological methods, since highly specific polyclonal antibodies were raised against these polysaccharides. Mycelium of P. variotii CBS 990.73A was stained by indirect immunofluorescence. The polysaccharides studied in this work differ from the one described for species from section Isarioidea, and this is another indication of the heterogeneity of the genus Paecilomyces.

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Immunological relationship of extra-cellular polysaccharide antigens produced by different mould species

Cited by 50 publications

References 13 publications

Isolation and electrophoretic analysis of surface proteins of the zygomycete Absidia glauca

Isolation and electrophoretic analysis of surface proteins of the zygomycete Absidia glauca

Imunoensaios: uma alternativa para a detecção de fungos toxigênicos em alimentos

Galactomannans from the cell walls of species of Paecilomyces sect. Paecilomyces and their teleomorphs as immunotaxonomic markers

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